关键词: 蛋白质组学, 小麦, 抗条锈, 二维凝胶电泳, 肽质量指纹图谱

Abstract:

【Objective】 To investigate the biochemical changes of wheat resistance to stripe rust after infected with the stripe rust, a resistive line of wheat (Taichung29*6/Yr5) infected with stripe rust CY32 was compared to mock-inoculated wheat by comparative proteomics technology. 【Method】 Proteins were extracted from leaves infected with a virulent race of stripe rust CY32 and control group after 48 hours of inoculation, and then separated by using two-dimensional electrophoresis (2-DE). The differential protein spots were selected for MALDI-TOF MS analysis and database searching. 【Result】 By using 2-DE，2 uniquely expressed spots, 5 up-regulated spots and 6 down-regulated spots were observed in Taichung29*6/Yr5 leaves inoculated with stripe rust CY32. Total of 11 proteins, including pathogenesis-related homeodomain protein, beta-glucosidase, glutathione transferase etc, were identified. 【Conclusion】 Through analysis of protein function , it is presumed that these differential proteins are correlated with the infextation of stripe rust race CY32.

Key words: proteomics, wheat, stripe rust resistance, two-dimensional gel electrophoresis, PMF