关键词: 玉米, 河南省, 地方品种, SSR, 遗传多样性

Abstract:

【Objective】 To provide a reasonable further improved program, genetic diversity of maize landrace in Henan was analyzed. 【Method】 Genetic diversity of 88 maize landraces was analyzed by phenotypes and SSR genotype in this research. 【Result】 These maize germplasms varied significantly in all of the measured morphological traits. Phenotype cluster analysis showed the 88 accessions were divided into seven groups, and most of the landraces were clustered into one group. However, a few varieties could be clustered into another single group. 2-10 polymorphic fragments were amplified stably by each SSR marker. Total 198 alleles were detected at 40 loci and the average number of alleles was 4.95. Genetic dissimilarity coefficients ranged from 0.63 to 0.89. These maize germplasms were also could be clustered into seven groups, and most of them could be clustered into one group. The relationship between morphological and SSR-based distance was significant and positive (r=0.78, P=0.01) by Mental test.【Conclusion】 The results showed that the genetic resource of Henan maize landraces is narrow and single, the genetic diversity is low, and only a few landraces are divided into single groups. So the improvement and conservation of maize landrace in Henan should be made according to the targest.

Key words: maize (Zea mays L.), Henan Province, landrace, SSR, genetic diversity