中国农业科学 ›› 2009, Vol. 42 ›› Issue (3): 1084-1090 .doi: 10.3864/j.issn.0578-1752.2009.03.042

• 兽医 • 上一篇    下一篇

单色光对肉雏鸡小肠黏膜形态结构的影响

  

  1. 中国农业大学动物医学院
  • 收稿日期:2008-03-06 修回日期:2008-06-11 出版日期:2009-03-10 发布日期:2009-03-10
  • 通讯作者: 陈耀星

Effects of Monochromatic Light on Structure of Small Intestinal Mucosa in Broilers

  

  1. 中国农业大学动物医学院
  • Received:2008-03-06 Revised:2008-06-11 Online:2009-03-10 Published:2009-03-10
  • Contact: CHEN Yao-xing

摘要:

【目的】研究单色光对肉雏鸡小肠黏膜形态结构的影响,为阐明单色光影响肉雏鸡生长发育的作用机理提供形态学依据。【方法】采用LED(Light-emitting diodes)灯作为光源,选用刚出壳AA肉公雏120羽,随机分为4组,分别提供红(660 nm)、绿(560 nm)、蓝(480 nm)和白(400~700 nm)4种光源,每个处理设6个重复,每个重复5只,人工光照光强度均为15 lx,光照时间23 h,试验期7 d;分别于0、7 d时每个重复随机选取1只,利用组织化学染色方法及免疫组织化学方法检测肉鸡十二指肠、空肠、回肠的黏膜结构变化、杯状细胞数量以及空肠肠腺上皮细胞的增殖细胞核抗原(PCNA)的表达。【结果】7日龄时,绿光组各项检测指标比其它光色组明显增高,与红光组相比,绿光组能够促进雏鸡空肠肠腺上皮细胞的增殖(115.5%,P<0.05),提高十二指肠、空肠和回肠绒毛上皮的杯状细胞数量(分别为71.8%,35.8%和27.2%,P<0.05),增加十二指肠、空肠和回肠绒毛高度(分别为207.9,93.9和63.9 μm,P<0.05),黏膜厚度(分别为262.2,184.6和185.6 μm,P<0.05)以及绒毛高度/隐窝深度比值(分别为18.9%,54.5%和77.8%,P<0.05)。【结论】在15 lx光强度下,肉鸡生长早期(0~7 d)选用绿光照明,可不同程度地改善肉鸡小肠黏膜结构,提高小肠对营养物质的吸收能力,从而促进肉鸡生长发育。

关键词: 单色光, 肉鸡, 小肠黏膜, 杯状细胞, 肠腺上皮细胞

Abstract:

【Objective】 This study was conducted to investigate the effect of various monochromatic lights on small intestine mucosal morphology and structure of chicken broilers, and to provide morphological data about the mechanism of monochromatic light action on development of broilers. 【Method】 A total of 120 day-old Arbor Acre male broilers were exposed to red (660 nm, R), green (560 nm, G), blue (480 nm, B), and white (400-700 nm, W) light from light emitting diode (LED) lamps for 7 d, respectively. All light sources were equalized under 15 lx light intensity for 23 h daily. There were six replication pens for each light treatment and 5 birds per pen. Then at the 0 and 7 day old, 1 chick from each pen was randomly sampled and killed. The proliferating cell nuclear antigen (PCNA) positive cells expression in jejunal gland epithelium of broilers, and intestinal goblet cells numbers, villous height, mucosa thickness, as well as the ratio of height villous to intestinal crypt (V/C) of broiler duodenum, jejunum and ileum were examined by histochemistry staining and immunohistochemistry methods. 【Result】Compared with other light groups, GL group increased obviously the related detection index at 7 day old. Compared with RL, GL increased broilers jejunal gland epithelium cells proliferation (115.5%, P<0.05); GL increased the number of goblet cells of the duodenum, jejunum, and ileum of broilers by 71.8%, 35.8% and 27.2%, respectively (P<0.05); increased the villus height of the duodenum, jejunum, and ileum of broilers by 207.9, 93.9 and 63.9μm, respectively (P<0.05); increased the mucosal thickness of the duodenum, jejunum, and ileum of broilers by 262.2, 184.6 and 185.6μm, respectively (P<0.05); as well as the ratio of V/C of the duodenum, jejunum, and ileum of broilers by 18.9%, 54.5% and 77.8%, respectively (P<0.05). 【Conclusion】 The small intestine mucosal structure would be improved to an extent in chicken broilers when they were illuminated with green light at early growth stage (0-7 d) of broiler reared under 15 lx light intensity, accordingly improving the absorption function of small intestine, and accelerating broilers growth.

Key words: monochromatic light, broilers, small intestine mucosa, goblet cells, intestinal gland epithelium cells