关键词: 小麦, α-醇溶蛋白基因, 序列分析

Abstract: The full coding regions of α-gliadin genes were amplified from wheat variety “Chuannong 16” by using the primer pairs, which were designed according to the known α-gliadin gene sequences. The amplified DNA fragments were separated and recovered from agrose gel, subsequently ligated into pMD18-T vector, and then transformed into E. coli strain DH5α. Four positive clones were screened out and sequenced. Four gliadin gene sequences were obtained, designated as Gli2-CN16-9, Gli2-CN16-12, Gli2-CN16-14 and Gli2-CN16-6, and the GenBank accession numbers were DQ246446, DQ246447, DQ246448 and DQ246449, respectively. Among which, the coding regions of Gli2-CN16-9, Gli2-CN16-12 and Gli2-CN16-14 are 861bp, 870bp and 900bp, respectively, and could encode three mature proteins with 286, 289 and 299 amino acid residues, respectively. Gli2-CN16-6 is a pseudogene due to that two stop codons are in its coding region. Multiple sequence alignment analysis suggested that the cloned gliadin gene sequences have the higher similarity in their structure with the known α-gliadin genes, whereas they have much difference with the γ- and ω-gliadin genes.

Key words: wheat, α-gliadin gene, sequence analysis