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Special Issue:
油料作物合辑Oil Crops
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| Fine mapping and genetic analysis of resistance genes, Rsc18, against soybean mosaic virus |
| LIU Sang-lin1, 2, CHENG Yan-bo1, 2, MA Qi-bin1, 2, LI Mu1, 2, 3, JIANG Ze1, 2, XIA Qiu-ju4, NIAN Hai1, 2 |
1 The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, P.R.China
2 The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China
3 Maize Research Institute, Jilin Academy of Agricultural Sciences, Gongzhuling 130033, P.R.China
4 Beijing Genomics Institute (BGI)-Shenzhen, Shenzhen 518086, P.R.China |
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摘要
大豆花叶病毒（SMV）病是一种世界性病害，严重影响大豆（Glycine max（L.）Merr.）产量和品质。SC18是华南地区主要的大豆花叶病毒株系之一，但其抗性遗传机制尚不完全清楚。本研究利用中黄24（抗性）和华夏3号（感性）的杂交后代F1，F2和168个F11重组自交系（RILs）群体，利用高密度遗传图谱对抗病品种中黄24的SC18株系抗性基因进行精细定位，并分析其遗传机理。根据F2（3R:1S）和重组自交系（RILs）群体（1R:1S）的分离比例，单个显性基因调控中黄24对SC18的抗性。复合区间作图法（CIM）将抗性基因位点定位在第13号染色体上415.357 kb区域，LOD值为37.43，表型变异解释率达62.01%。根据定位区间内基因的功能注释，鉴定了三个可能与SC18抗性有关的候选基因，包括一个NBS-LRR型基因和两个丝氨酸/苏氨酸蛋白基因。与对照相比，这些基因在抗性材料中表达上调，其中NBS-LRR型基因在亲本外显子非同义SNV区域存在单碱基替换。这项研究为大豆分子抗病育种以及SC18抗性基因定位和功能验证提供参考。
Abstract Soybean mosaic virus (SMV) affects seed quality and production of soybean (Glycine max (L.) Merr.) worldwide. SC18 is one of the dominant SMV strains in South China, and accession Zhonghuang 24 displayed resistance to SC18. The F1, F2 and 168 F11 recombinant inbred lines (RILs) population derived from a hybridization between Zhonghuang 24 (resistant, R) and Huaxia 3 (susceptible, S) were used in this study. According to the segregation ratios of the F2 generation (3R:1S) and the recombinant inbred lines (RILs) population (1R:1S), one dominant locus may regulate the resistance to SC18 in Zhonghuang 24. By using composite interval mapping (CIM), Rsc18 was mapped to a 415.357-kb region on chromosome 13. Three candidate genes, including one NBS-LRR type gene and two serine/threonine protein type genes, were identified according to the genetic annotations, which may be related to the resistance to SC18. The qRT-PCR demonstrated that these genes were up-regulated in the R genotype compared to the control. In conclusion, the findings of this research enhanced the understanding about the R genes at the Rsc18 locus. Moreover, our results will provide insights for designing molecular markers to improve marker-assisted selection and developing new varieties with resistance to SC18.
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Received: 03 July 2020
Accepted: 24 November 2020
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| Fund: This work was supported by the projects of the Key-Areas Research and Development Program of Guangdong Province, China (2020B020220008), the China Agriculture Research System of MOF and MARA (CARS-04-PSO9), the Major Project of New Varieties Cultivation of Genetically Modified Varieties, China (2016ZX08004002-007), the National Key R&D Program of China (2017FYD0101500), and the National Natural Science Foundation of China (31971966). |
| About author: Correspondence NIAN Hai, Tel: +86-20-85280203, E-mail: hnian@scau.edu.cn |
Cite this article:
LIU Sang-lin, CHENG Yan-bo, MA Qi-bin, LI Mu JIANG Ze, XIA Qiu-ju, NIAN Hai.
2022.
Fine mapping and genetic analysis of resistance genes, Rsc18, against soybean mosaic virus. Journal of Integrative Agriculture, 21(3): 644-653.
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