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| Fine mapping of a novel wax crystal-sparse leaf3 gene in rice |
| GONG Hong-bing1, 2*, ZENG Sheng-yuan2*, XUE Xiang1, ZHANG Ya-fang1, CHEN Zong-xiang1, ZUO Shi-min1, LI Chuang2, LIN Tian-zi2, JING De-dao2, YU Bo2, QIAN Hua-fei2, PAN Xue-biao1, SHENG Sheng-lan2 |
1 Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics, Ministry of Education/Agricultural College, Yangzhou University, Yangzhou 225009, P.R.China
2 Zhenjiang Agricultural Research Institute, Jurong 212400, P.R.China |
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Abstract Cuticular wax plays an important role in protecting plants against water loss and pathogen infection and in the adaptations to environmental stresses. The genetic mechanism of the biosynthesis and accumulation of epicuticular wax in rice remains largely unknown. Here, we show a spontaneous mutant displaying wax crystal-sparse leaves and decreased content of epicuticular wax that was derived from the cytoplasmic male sterility (CMS) restorer line Zhenhui 714. Compared with the wild type Zhenhui 714, the mutant exhibited hydrophilic features on leaf surface and more sensitivity to drought stress. The mutation also caused lower grain number per panicle and thousand grain weight, leading to the decline of yield. Genetic analysis indicates that the mutation is controlled by a single recessive gene, named wax crystal-sparse leaf3 (wsl3). Using segregation populations derived from crosses of mutant/Zhendao 88 and mutant/Wuyujing 3, respectively, the wsl3 gene was fine-mapped to a 110-kb region between markers c3-16 and c3-22 on chromosome 3. According to the rice reference genome and gene analysis, we conclude that a novel gene/mechanism involved in regulation of rice cuticular wax formation.
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Received: 07 March 2016
Accepted:
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| Fund: This research was supported by grants from Jiangsu Province Self-innovation Program, China (CX (13)5073), the Natural Science Foundation of Jiangsu Province of China (BK20141291) and the Jiangsu 333 Program, China (BRA2014170). |
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Corresponding Authors:
PAN Xue-biao, Tel: +86-514-87972136, E-mail: shuidao@yzu.edu.cn
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| About author: GONG Hong-bing, E-mail: hongbinggong973@sina.com |
Cite this article:
GONG Hong-bing, ZENG Sheng-yuan, XUE Xiang, ZHANG Ya-fang, CHEN Zong-xiang, ZUO Shi-min, LI Chuang, LIN Tian-zi, JING De-dao, YU Bo, QIAN Hua-fei, PAN Xue-biao, SHENG Sheng-lan .
2017.
Fine mapping of a novel wax crystal-sparse leaf3 gene in rice. Journal of Integrative Agriculture, 16(02): 497-502.
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