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| Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves |
| MA Tao, TU Yan, ZHANG Nai-feng, GUO Jiang-peng, DENG Kai-dong, ZHOU Yi, YUN Qiang, DIAO Qi-yu |
1、Feed Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Feed Biotechnology, Ministry ofAgriculture, Beijing 100081, P.R.China
2、Beijing General Station of Animal Husbandry, Beijing 100081, P.R.China |
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摘要 This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves ((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet (control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed (dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment (P>0.05), the average daily gain (ADG) and gain-to-feed ratios were higher (P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and phosphorus (P) (P<0.05) and the ratio of intestinal villous height to crypt depth (V/C) (P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein (TP), albumin (ALB), serum urea nitrogen (SUN) and glucose (GLU) were observed (P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased (P<0.05) the serum concentrations of triglycerides (TG) and total cholesterol (TC). The serum concentration of immunoglobulin G (IgG) and immunoglobulin M (IgM) increased quadratically (P<0.05), whereas the serum concentration of immunoglobulin A (IgA) was unaffected by dietary treatments (P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase (ALP) (P<0.05) compared with the control group. The lysozyme (LYZ) concentration increased quadratically (P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves.
Abstract This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves ((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet (control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed (dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment (P>0.05), the average daily gain (ADG) and gain-to-feed ratios were higher (P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter (DM), crude protein (CP), ether extract (EE), and phosphorus (P) (P<0.05) and the ratio of intestinal villous height to crypt depth (V/C) (P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein (TP), albumin (ALB), serum urea nitrogen (SUN) and glucose (GLU) were observed (P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased (P<0.05) the serum concentrations of triglycerides (TG) and total cholesterol (TC). The serum concentration of immunoglobulin G (IgG) and immunoglobulin M (IgM) increased quadratically (P<0.05), whereas the serum concentration of immunoglobulin A (IgA) was unaffected by dietary treatments (P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase (ALP) (P<0.05) compared with the control group. The lysozyme (LYZ) concentration increased quadratically (P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves.
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Received: 14 March 2014
Accepted:
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| Fund: This study was supported by the Earmarked Fund for Beijing Dairy Industry Innovation Team, the Beijing Key Technology for Early Weaning of Calves, and the National Key Technology R&D Program of China (2012BAD12B06). |
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Corresponding Authors:
DIAO Qi-yu, Tel: +86-10-82106055, E-mail: diaoqiyu@caas.cn
E-mail: diaoqiyu@caas.cn
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| About author: MA Tao, E-mail: matao0229@gmail.com;* These authors contributed equally to this study. |
Cite this article:
MA Tao, TU Yan, ZHANG Nai-feng, GUO Jiang-peng, DENG Kai-dong, ZHOU Yi, YUN Qiang, DIAO Qi-yu.
2015.
Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Journal of Integrative Agriculture, 14(4): 749-757.
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| Amábile-Cuevas C F, Cárdenas-García M, Ludgar M. 1995. Antibiotic-resistance. American Scientist, 83, 320–329. AOAC (Association of Official Analytical Chemists). 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Washington, D.C. Baldwin R L V I, Cleod K R M, Klotz J L, Heitmann R N. 2004. Rumen development, intestinal growth and hepatic metabolism in the pre- and postweaning ruminant. Journal of Dairy Science, 87(Suppl. E), E55–E65. Chen K L, Weng B C, Chang M T, Liao Y H, Chen T T, Chu C. 2008. Direct enhancement of the phagocytic and bactericidal capability of abdominal macrophage of chicks by beta-1,3-1,6-glucan. Poultry Science, 87, 2242–2249. Chen M Y, Yang H S, Delaporte M, Zhao S J, Xing K. 2007. Immune responses of the scallop Chlamys farreri after air exposure to different temperatures. Journal of Experimental Marine Biology and Ecology, 345, 52–60. Cox C M, Stuard L H, Kim S, McElroy A P, Bedford M R, Dalloul R A. 2010. Performance and immune responses to dietary beta-glucan in broiler chicks. Poultry Science, 89, 1924–1933. Cruywagen C W, Jordaan I, Venter L. 1996. Effect of Lactobacillus acidophilus supplementation of milk replacer on preweaning performance of calves. Journal of Dairy Science, 79, 483–486. Donovan D C, Franklin S T, Chase C C, Hippen A R. 2002. Growth and health of Holstein calves fed milk replacers supplemented with antibiotics or Enteroguard. Journal of Dairy Science, 85, 947–950. Doornenbal H, Tong A K, Murray N L. 1988. Reference values of blood parameters in beef cattle of different ages and stages of lactation. Canadian Journal of Veterinary Research-revue, 52, 99–105. Eicher R, Liesegang A, Bouchard E, Tremblay A. 1999. Effect of cow-specific factors and feeding frequency of concentrate on diurnal variations of blood metabolites in dairy cows. American Journal of Veterinary Research, 60, 1493–1499. Eicher S D, McKee C A, Carroll J A, Pajor E A. 2006. Supplemental vitamin C and yeast cell wall beta-glucan as growth enhancers in newborn pigs and as immunomodulators after an endotoxin challenge after weaning. Journal of Animal Science, 84, 2352–2360. Eicher S D, Wesley I V, Sharma V K, Johnson T R. 2010. Yeast cell-wall products containing beta-glucan plus ascorbic acid affect neonatal Bos taurus calf leukocytes and growth after a transport stressor. Journal of Animal Science, 88, 1195–1203. Hahn T W, Lohakare J D, Lee S L, Moon W K, Chae B J. 2006. Effects of supplementation of beta-glucans on growth performance, nutrient digestibility, and immunity in weanling pigs. Journal of Animal Science, 84, 1422–1428. Harris H. 1990. The human alkaline phosphatases: What we know and what we don’t know. Clinica Chimica Acta, 186, 133–150. Hill T M, Bateman H N, Aldrich J M, Schlotterbeck R L. 2009a. Effects of fat concentration of a high-protein milk replacer on calf performance. Journal of Dairy Science, 92, 5147–5153. Hill T M, Bateman H N, Aldrich J M, Schlotterbeck R L. 2009b. Optimizing nutrient ratios in milk replacers for calves less than five weeks of age. Journal of Dairy Science, 92, 3281–3291. Huth M, Dongowski G, Gebhardt E, Flamme W. 2000. Functional properties of dietary fibre enriched extrudates from barley. Journal of Cereal Science, 32, 115–128. Jonker D, Hasselwander O, Tervila-Wilo A, Tenning P P. 2010. 28-Day oral toxicity study in rats with high purity barley beta-glucan (GlucagelTM). Food and Chemical Toxicology, 48, 422–428. Kalra S, Joad S. 2000. Effect of dietary barley beta-glucan on cholesterol and lipoprotein fractions in rats. Journal of Cereal Science, 31, 141–145. Keenan J M, Goulson M, Shamliyan T, Knutson N, Kolberg L, Curry L. 2007. The effects of concentrated barley beta-glucan on blood lipids in a population of hypercholesterolaemic men and women. British Journal of Nutrition, 97, 1162–1168. Khuntia A, Chaudhary L C. 2002. Performance of male crossbred calves as influenced by substitution of grain by wheat bran and the addition of lactic acid bacteria to diet. Asian-Australasian Journal of Animal Sciences, 15, 188–194. Kokoshis P L, Williams D L, Cook J A, Luzio N R D. 1978. Increased resistance to Staphylococcus aureus infection and enhancement in serum lysozyme activity by glucan. Science, 199, 1340–1342. Lesmeister K E, Tozer P R, Heinrichs A J. 2004. Development and analysis of a rumen tissue sampling procedure. Journal of Dairy Science, 87, 1336–1344. Li H, Diao Q Y, Zhang N F, Fan Z Y. 2008. Growth, nutrient utilization and amino acid digestibility of dairy calves fed milk replacers containing different amounts of protein in the preruminant period. Asian-Australasian Journal of Animal Sciences, 21, 1151–1158. Li J, Li D F, Xing J J, Cheng Z B, Lai C H. 2006. Effects of β-glucan extracted from Saccharomyces cerevisiae on growth performance, and immunological and somatotropic responses of pigs challenged with Escherichia coli lipopolysaccharide. Journal of Animal Science, 84, 2374–2381. Li S. 1992. The effect of dietary level of protein, fiber and fat on amino acid digestibility in early-weaned pigs. MSc thesis, University of Alberta, Edmonton, Canada. Lin T T, Xing J, Jiang J W, Tang X Q, Zhan W B. 2011. Beta-glucan-stimulated activation of six enzymes in the haemocytes of the scallop Chlamys farreri at different water temperatures. Aquaculture, 315, 213–221. Mao X F, Piao X S, Lai C H, Li D F, Xing J J, Shi B L. 2005. Effects of β-glucan obtained from the Chinese herb Astragalus membranaceus and lipopolysaccharide challenge on performance, immunological, adrenal, and somatotropic responses of weanling pigs. Journal of Animal Science, 83, 2775–2782. Marsh M B, Rice C D. 2010. Development, characterization, and technical applications of a fish lysozyme-specific monoclonal antibody (mAb M24-2). Comparative Immunology Microbiology and Infectious Diseases, 33, e15–e23. McKee C A, Eicher S D, Johnson T R. 2000. Ascorbic acid and a beta-glucan product from Saccharomyces cerevisiae influence on dairy calf well-being. Journal of Dairy Science, 83(Suppl. 1), 134. Mohri M, Sharifi K, Eidi S. 2007. Hematology and serum biochemistry of Holstein dairy calves: age related changes and comparison with blood composition in adults. Research in Veterinary Science, 83, 30–39. van Nevel C J, Decuypere J A, Dierick N, Molly K. 2003. The influence of Lentinus edodes (Shiitake mushroom) preparations on bacteriological and morphological aspects of the small intestine in piglets. Archiv für Tierernahrung, 57, 399–412. Ortigues I, Martin C, Durand D, Vermorel M. 1995. Circadian changes in energy expenditure in the preruminant calf: Whole animal and tissue level. Journal of Animal Science, 73, 552–564. Rook G A, Brunet L R. 2005. Microbes, immunoregulation, and the gut. Gut, 54, 317–320. SAS Institute. 2005. SAS OnlineDoc® 9.1. SAS Institute, Cary, NC. Seal C J, Reynolds C K. 1993. Nutritional implications of gastrointestinal and liver metabolism in ruminants. Nutrition Research Reviews, 6(Suppl.), 185–208. Soltanian S, Stuyven E, Cox E, Sorgeloos P, Bossier P. 2009. Beta-glucans as immunostimulant in vertebrates and invertebrates. Critical Reviews in Microbiology, 35, 109–138. Studnicka M, Siwicki A, Ryka B. 1986. Lysozyme level in carp (Cyprinus carpio L.). Bamidgeh, 38, 22–25. Tang X Y, Gao J S, Yuan F, Zhang W X, Shao Y J, Sakurai F, Li Z D. 2011. Effects of Sophy beta-glucan on growth performance, carcass traits, meat composition, and immunological responses of Peking ducks. Poultry Science, 90, 737–745. Wang Z, Guo Y, Yuan J, Zhang B. 2008. Effect of dietary beta- 1,3/1,6-glucan supplementation on growth performance, immune response and plasma prostaglandin E-2, growth hormone and ghrelin in weanling piglets. Asian-Australasian Journal of Animal Sciences, 21, 707–714.Wood P J, Weisz J, Blackwell B A. 1994. Structural studies of (1→3),(1→4)-beta-D-glucans by 13C-nuclear magnetic resonance spectroscopy and by rapid analysis of cellulose-like regions using high-performance anion-exchange chromatography of oligosaccharides released by lichenase. Cereal Chemistry, 71, 301–307. Zhang B, Guo Y, Wang Z. 2008. The modulating effect of beta- 1,3/1,6-glucan supplementation in the diet on performance and immunological responses of broiler chickens. Asian- Australasian Journal of Animal Sciences, 21, 237–244. |
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