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| Characterization of MicroRNA* Species in Peking Duck Skin |
| ZHANG Li, XIE Xiu-juan, JIA Shan-gang, XIAO Mei, LIN Shu-dai, AN Li-long, LUO Wen, JIA Xinzheng, NIE Qing-hua , ZHANG Xi-quan |
1.Agricultural College, Guangdong Ocean University, Zhanjiang 524088, P.R.China
2.Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, Guangzhou 510642, P.R.China
3.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100029, P.R.China |
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摘要 A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3´-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.
Abstract A substantial fraction of miRNA* species are conserved in animals and can repress activities of target genes. This study aims to investigate the miRNA* species in duck skin by using Solexa sequencing. We obtained a total of 96 miRNA* species in two skin small RNA libraries and identified 56 miRNA/miRNA* (miR/miR*) pairs. Nucleotide bias of miRNA* indicated that the priority was C>A>U>G for the first nucleotide and U>C>A>G for the last nucleotide. Comparison analyses showed that 3´-U accounted for a higher proportion in the 56 miR/miR* pairs. Among the top 20 expressed miRNA* species, 17 were shared by two libraries and most of the miRNA* species were highly conservative, especially in the “seed region”. miR-199a* were expressed highly in our samples, which was also previously shown abundant in mouse hair follicle. Furthermore, four miRNA* species were predicted to target their genes in signal pathways of feather follicle development and feather morphogenesis despite very low levels.
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Received: 11 December 2012
Accepted:
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| Fund: This work was supported by the Open Fund of Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, South China Agricultural University, China (201004). |
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Corresponding Authors:
Correspondence ZHANG Xi-quan, Tel/Fax: +86-20-85285703, E-mail: xqzhang@scau.edu.cn
E-mail: xqzhang@scau.edu.cn
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| About author: ZHANG Li, E-mail: zhangli761101@163.com |
Cite this article:
ZHANG Li, XIE Xiu-juan, JIA Shan-gang, XIAO Mei, LIN Shu-dai, AN Li-long, LUO Wen, JIA Xinzheng, NIE Qing-hua , ZHANG Xi-quan.
2013.
Characterization of MicroRNA* Species in Peking Duck Skin. Journal of Integrative Agriculture, 12(9): 1614-1619.
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