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| Effects of Pesticide Applications on the Biochemical Properties of Transgenic cry 2A Rice and the Life History Parameters of Nilaparvata lugens Stål (Homptera: Delphacidae) |
| SHI Zhao-peng, DU Shang-gen, YANG Guo-qing, LU Zhen-zhen , WU Jin-cai |
| Horticulture and Plant Protection College, Yangzhou University, Yangzhou 225009, P.R.China |
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摘要 Effects of transgenic Bt rice on non-target pests following pesticide applications plays an important role in evaluating the ecological safety of transgenic rice. Changes of the life history parameters of laboratory population of Nilaparvata lugens feeding on transgenic Bt rice T2A-1 and its parental line cv. MH63 as the control, and the contents of oxalic acid and soluble sugar following three pesticides (triazophos, jinggangmycin and chlorantraniliprole) treatments were investigated in this paper. Results showed that the population parameters of N. lugens and the physiological and biochemical parameters of rice did not differ significantly between T2A-1 and MH63 without pesticide application. But, the emergence rate (ER), the nymphal number of next generation (NN), and the index of population trend (IP) on T2A-1 treated with 10 mg L-1 of triazophos were significantly higher than those on MH63, respectively, while the hatchability (HB) on T2A-1 treated with the three pesticides were obviously lower than those on MH 63. Furthermore, the content of oxalic acid in MH63 treated with 20 mg L-1 triazophos and, 40 and 80 mg L-1 chlorantraniliprole was significantly higher than that in T2A-1, while the content of soluble sugar in T2A-1 treated with 400 mg L-1 jinggangmycin was significantly higher than that in MH63. The results above indicated that triazophos in a low dose could cause a more favor of T2A-1 to population growth of N. lugens compared with MH 63.
Abstract Effects of transgenic Bt rice on non-target pests following pesticide applications plays an important role in evaluating the ecological safety of transgenic rice. Changes of the life history parameters of laboratory population of Nilaparvata lugens feeding on transgenic Bt rice T2A-1 and its parental line cv. MH63 as the control, and the contents of oxalic acid and soluble sugar following three pesticides (triazophos, jinggangmycin and chlorantraniliprole) treatments were investigated in this paper. Results showed that the population parameters of N. lugens and the physiological and biochemical parameters of rice did not differ significantly between T2A-1 and MH63 without pesticide application. But, the emergence rate (ER), the nymphal number of next generation (NN), and the index of population trend (IP) on T2A-1 treated with 10 mg L-1 of triazophos were significantly higher than those on MH63, respectively, while the hatchability (HB) on T2A-1 treated with the three pesticides were obviously lower than those on MH 63. Furthermore, the content of oxalic acid in MH63 treated with 20 mg L-1 triazophos and, 40 and 80 mg L-1 chlorantraniliprole was significantly higher than that in T2A-1, while the content of soluble sugar in T2A-1 treated with 400 mg L-1 jinggangmycin was significantly higher than that in MH63. The results above indicated that triazophos in a low dose could cause a more favor of T2A-1 to population growth of N. lugens compared with MH 63.
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Received: 02 November 2012
Accepted:
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| Fund: This work was funded by the National Science and Technology Major Project of the Ministry of Science and Technology of China (2009ZX08011-009B) and the Scientific Innovation Research of College Graduate in Jiangsu Province, China (CXZZ11-0986). |
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Corresponding Authors:
Correspondence YANG Guo-qing, Tel: +86-514-87979246, E-mail: gqyang@yzu.edu.cn
E-mail: gqyang@yzu.edu.cn
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Cite this article:
SHI Zhao-peng, DU Shang-gen, YANG Guo-qing, LU Zhen-zhen , WU Jin-cai.
2013.
Effects of Pesticide Applications on the Biochemical Properties of Transgenic cry 2A Rice and the Life History Parameters of Nilaparvata lugens Stål (Homptera: Delphacidae). Journal of Integrative Agriculture, 12(9): 1606-1613.
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| [1]Bernal C C, Aguda R M, Cohen M B. 2002. Effect of ricelines transformed with Bacillus thuringiensis toxingenes on the brown planthopper and its predatorCyrtorhinus lividipennis. Entomologia Experimentaliset Applicata, 102, 21-28[2]Cabauatan P Q, Cabunagan R C, Choi I R. 2009. Rice virusestransmitted by the brown planthopper Nilaparvatalugens St錶. In: Heong K L, Hardy B, eds., Planthoppers:New Threats to the Sustainability of Intensive RiceProduction Systems in Asia. Los Ba駉s, Philippines.pp. 357-368[3]Chen M, Liu Z C, Ye G Y, Shen Z C, Hu C, Peng Y F,Altosaar I, Shelton A M. 2007. Impacts of transgeniccry1Ab rice on non-target planthoppers and their mainpredator Cyrtorhinus lividipennis (Hemiptera: Miridae)-A case study of the compatibility. Biological Control,42, 242-250[4]Chen M, Ye G Y, Yao H W, Hu C, Shu Q Y. 2004. Evaluationof the impact of insect-resistant transgenic rice on thefeeding and oviposition behavior of its non-targetinsect, the brown planthopper, Nilaparvata lugens(Homptera: Delphacidae). Scientia Agricultura Sinica,37, 222-226[5](in Chinese)Fu Q, Wang F, Li D H, Yao Q, Lai F X, Zhang Z T. 2003.Effects of insect-resistant transgenic rice lines MSAand MSB on non-target pests Nilaparvata lugens andSogatella fucifera. Acta Entomologia Sinica, 46, 697-704 (in Chinese)[6]Gao Y L, Hu Y, Fu Q, Zhang J, Oppert B, Lai F X, Peng Y F,Zhang Z T. 2010. Screen of Bacillus thuringiensistoxins for transgenic rice to control Sesamia inferensand Chilo suppressalis. Journal of InvertebratePathology, 105, 11-15[7]Ge L Q, Wu J C, Zhao K F, Chen Y, Yang G Q. 2010. Inductionof Nlvg and suppression of Nljhe gene expression inNilaparvata lugens (St錶) (Hemiptera: Delphacidae)adults females and males exposed to two insecticides.Pesticide Biochemistry and Physiology, 98, 269-278[8]Han Y, Xu X L, Ma W H, Yuan B Q, Wang H, Liu F Z, WangM Q, Wu G, Hua H X. 2011. The influence of transgeniccry1Ab/cry1Ac, cry1C and cry2A rice on non-targetplanthoppers and their main predators under fieldconditions. Agricultural Sciences in China, 10, 1739-1747[9]High S M, Cohen M B, Shu Q Y, Altosaar I. 2004. Achievingsuccessful deployment of Bt rice. Trends in PlantScience, 9, 286-292[10]Hu J H, Wu J C, Yin J L, Gu H N. 2010. Physiology ofinsecticide-induced stimulation of reproduction in therice brown planthopper (Nilaparvata lugens (St錶)):dynamics of protein in fat body and ovary.International Journal of Pest Management, 56, 23-30[11]Liu J L, Yu J F, Wu J C, Yin J L, Gu H N. 2008. Physiologicalresponses to Nilaparvata lugens in susceptible andresistant rice varieties: allocation of assimilates betweenshoots and roots. Journal of Economic Entomology,101, 384-390[12]Nagata T, Hayakawa T. 1998. Activity of aconitic acids andoxalic acid on brown planthopper, Nilaparvata lugens(St錶) and green rice leafhopper, Nephotettix cincticeps(Uhler). Japanese Journal of Applied Entomology andZoology, 42, 115-121[13]Poppy G. 2000. GM crops: environmental risks and nontargeteffects. Trends in Plant Science, 5, 4-6[14]Qiu Z H, Wu J C, Dong B, Li D H, Gu H N. 2004. Two-wayeffect of pesticides on zeatin riboside content in bothrice leaves and roots. Crop Protection, 23, 1131-1136[15]Schuler T H, Poppy G M, Kerry B R, Denholm I. 1999.Potential side effects of insect-resistant transgenicplants on arthropod natural enemies. Trends inBiotechnology, 17, 210-216[16]SPSS Inc. 2002. SPSS II for Mac OS X. SPSS Inc., Chicago,IL.Tan H, Ye G Y, Shen J H, Peng Y F, Hu C. 2006. Effects oftransgenic indica rice expressing a gene of cry1Ab withinsect resistance on the development and reproductionof nontarget pest, Sogatella furcifera (Homoptera:Delphacidae). Acta Phytophylacica Sinica, 33, 251-256(in Chinese)[17]Timmons A M, Charters Y M, Crawford J W, Burn D, ScottS E, Dubbels S J, Wilson N J, Robertson A, O乫Brien E T,Squire G, et al. 1996. Risks from transgenic crops.Nature, 380, 487.Tu J M, Zhang G A, Data K, Xu C G, He Y Q, Zhang Q F,Khush G S, Datta S K. 2000. Field performance oftransgenic elite commercial hybrid rice expressingBacillus thuringiensis 冃-endotoxin. NatureBiotechnology, 18, 1101-1104[18]Wang H R, Wu J C, Yang F, Geng J, Wang F. 2009. Life tableparameters of im idacloprid resistant and susceptiblepopulations of Nilaparvata lugens Stal (Homoptera:Delphacidae) under sublethal doses of insecticide. ActaEcologica Sinica, 29, 4753-4760[19](in Chinese)Wang Y M, Zhang G A, Du J P, Liu B, Wang M C. 2010.Influence of transgenic hybrid rice expressing a fusedgene derived from cry1Ab and cry1Ac on primary insectpests and rice yield. Crop Protection, 29, 128-133[20]Wu J C, Qiu H M, Yang G Q, Liu J L, Liu G J, Wilkins R M.2004. Effective duration of pesticide-inducedsusceptibility of rice to brown planthopper(Nilaparvata lugens Stal, Homoptera: Delphacidae),and physiological and biochemical changes in riceplants following pesticide application. InternationalJournal of Pest Management, 50, 55-62[21]Wu J C, Xu J X, Yuan S Z, Liu J L, Jiang Y H, Xu J F. 2001.Pesticide-induced susceptibility of rice to brownplanthopper Nilaparvata lugens. EntomologiaExperimentalis et Applicata, 100, 119-126[22]Ye G Y, Yao H W, Shu Q Y, Cheng X, Hu C, Xia Y W, Gao MW, Altosaar I. 2003. High levels of stable resistance intransgenic rice with a cry1Ab gene from Bacillust h u r i n g i e n s i s B e r l i n e r t o r i c e l e a f f o l d e r ,Cnaphalocrocis medinalis (Guenee) under fieldconditions. Crop Protection, 22, 171-178[23]Ye G Y, Shu Q Y, Yao H W, Cui H R, Cheng X Y, Hu C, XiaY W, Gao M W, Altosaar I. 2001. Field evaluation ofresistance of transgenic rice containing a syntheticcry1Ab gene from Bacillus thuringiensis Berliner totwo stem borers. Journal of Economic Entomology,94, 271-276[24]Zeng Y Y, Huang W K, Su L, Wu G, Zhuang J, Zhao W Y,Hua H X, Li J S, Xiao N W, Xiong Y F. 2012. Effects ofelevated CO2 on the nutrient compositions andenzymes activities of Nilaparvata lugens nymphs fedon rice plants. Science in China Series (C: LifeSciences), 55, 920-926[25]Zhang J M. 1997. Spectrophotometric analysis on oxaicacid. Journal of Anhui Institute of Mechanical andElectrical Engineering, 12, 31-35[26](in Chinese)Zhao K F, Shi Z P, Wu J C. 2011. Insecticide-inducedenhancement of flight capacity of the brownplanthopper, Nilaparvata lugens (Homptera:Delphacidae). Crop Protection, 30, 476-482[27]Zhou X, Cheng J A, Lou Y G. 2006. Effects of transgeniccry1Ab rice on population development of the whitebackedplanthopper, Sogatella furcifera (Horvath)(Homoptera: Delphacidae). Acta Entomologica Scinica,49, 786-791 (in Chinese) |
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