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Journal of Integrative Agriculture  2013, Vol. 12 Issue (6): 1042-1048    DOI: 10.1016/S2095-3119(13)60324-X
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Effects of Destruxin A on Hemocytes Morphology of Bombyx mori
 FAN Ji-qiao, CHEN Xiu-run , HU Qiong-bo
College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China
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摘要  Destruxin A (DA), a kind of cyclo-hexadepsipeptide isolated from entomopathogenic fungus, Metarhizium anisopliae, is an inhibitor of insect’s immunity. But its mechanism has not been clarified yet. In this study, the effects of DA on morphologic changes of in vivo and in vitro hemocytes of silkworm, Bombyx mori, were investigated by means of inverted phase contrast microscopy (IPCM), fluorescence microscopy (FCM) and environmental scanning electron microscopy (ESEM). The results indicated that DA was cytotoxic to granulohemocytes (GR) and plasmatocytes (PL). The LC50 values of DA against in vitro GR and PL of silkworm were 68.77 and 84.11 μg mL-1, respectively. However, the hemocytes in vivo were more susceptible to DA, although at the extremely low dose of 10 μL of 12.5 μg mL-1 for each insect (i.e., 0.036 μg g-1 body weight, or approximately 0.25 μg mL-1 hemolymph), DA could induce obviously morphologic alterations of hemocytes in vivo. The results imply that there might be some factors in silkworm’s hemolymph, which influence the interaction of DA and hemocytes.

Abstract  Destruxin A (DA), a kind of cyclo-hexadepsipeptide isolated from entomopathogenic fungus, Metarhizium anisopliae, is an inhibitor of insect’s immunity. But its mechanism has not been clarified yet. In this study, the effects of DA on morphologic changes of in vivo and in vitro hemocytes of silkworm, Bombyx mori, were investigated by means of inverted phase contrast microscopy (IPCM), fluorescence microscopy (FCM) and environmental scanning electron microscopy (ESEM). The results indicated that DA was cytotoxic to granulohemocytes (GR) and plasmatocytes (PL). The LC50 values of DA against in vitro GR and PL of silkworm were 68.77 and 84.11 μg mL-1, respectively. However, the hemocytes in vivo were more susceptible to DA, although at the extremely low dose of 10 μL of 12.5 μg mL-1 for each insect (i.e., 0.036 μg g-1 body weight, or approximately 0.25 μg mL-1 hemolymph), DA could induce obviously morphologic alterations of hemocytes in vivo. The results imply that there might be some factors in silkworm’s hemolymph, which influence the interaction of DA and hemocytes.
Keywords:  destruxin       toxicity       hemocytes       silkworm  
Received: 11 September 2012   Accepted:
Fund: 

This study was funded by the National 863 Program of China (2012AA101505).

Corresponding Authors:  Correspondence HU Qiong-bo, Tel: +86-20-85280308, E-mail: hqbscau@126.com     E-mail:  hqbscau@126.com

Cite this article: 

FAN Ji-qiao, CHEN Xiu-run , HU Qiong-bo. 2013. Effects of Destruxin A on Hemocytes Morphology of Bombyx mori. Journal of Integrative Agriculture, 12(6): 1042-1048.

[1]Bains P S, Tewari J P. 1987. Purification, chemical characterization and host-specifity of the toxinproduced by Alternaria brassicae. Physiological andMolecular Plant Pathology, 30, 259-271

[2]Chen H C, Chou C K, Sun C M, Yeh S F. 1997. Suppressiveeffects of destruxin B on hepatitis B virus surfaceantigen gene expression in human hepatoma cells.Antiviral Research, 34, 137-144

[3]Dumas C, Matha V, Quiot J M, Vey A. 1996. Effects ofdestruxins, cyclic depsipeptide mycotoxins, on calciumbalance and phosphorylation of intracellular proteinsin lepidopteran cell lines. Comparative Biochemistryand Physiology, 114C, 213-219

[4]Gao Q, Jin K, Ying S H, Zhang Y, Xiao G, Shang Y, Duan Z,Hu X, Xie X Q, Zhou G, et al. 2011. Genome sequencingand comparative transcriptomics of the modelentomopathogenic fungi Metarhizium anisopliae andM. acridum. PLoS Genetics, 7, e1001264.Hinaje M, Ford M, Banting L. 2002. An investigation of theionophoric characteristics of destruxin A. Archives ofBiochemistry and Biophysics, 405, 73-77

[5]Hu Q B, Ren S X, Wu J H, Chang J M, Musa P D. 2006.Investigation of destruxin A and B from Metarhiziumstrains in China, and the optimization of culturalconditions for the strain MaQ10. Toxicon, 48, 491-498

[6]Hu Q B, Ren S X, An X C, Qian M H. 2007. Insecticidalactivity influence of destruxins on the pathogenicity ofPaecilomyces javanicus against Spodoptera litura.Journal of Applied Entomology, 131, 262-268

[7]Hu Q B, An X C, Jin F L, Freed S, Ren S X. 2009. Toxicitiesof destruxins against Bemisia tabaci and its naturalenemy, Serangium japonicium. Toxicon, 53, 115-121

[8]Kodaira Y. 1961. Toxic substances to insects, produced byAspergillus ochraceus and Oospora destructor.Agricultural Biology and Chemistry, 25, 261-262

[9]Kodaira Y. 1962. Studies on the new toxic substances toinsects, destruxin A and B, produced by Oosporadestructor. Agricultural Biology and Chemistry, 26,36-42

[10]Manachini B, Arizza V, Parrinello D, Parrinello N. 2011.Hemocytes of Rhynchophorus ferrugineus (Olivier)(Coleoptera: Curculionidae) and their response toSaccharomyces cerevisiae and Bacillus thuringiensis.Journal of Invertebrate Pathology, 106, 360-365

[11]Pedras M S C, Zaharia L I, Ward D E. 2002. The destruxins:synthesis, biosynthesis, biotransformation, andbiological activity. Phytochemistry, 59, 579-596

[12]Ruiz-Sanchez E, O’Donnell M J. 2012. Effects of themicrobial metabolite destruxin a on ion transport by thegut and renal epithelia of Drosophila melanogaster.Archives of Insect Biochemistry and Physiology, 80,109-122

[13]Ruiz-Sanchez E, Orchard I, Lange A B. 2010a. Effects of thecyclopeptide mycotoxin destruxin A on the Malpighiantubules of Rhodnius prolixus (Stål). Toxicon, 55, 1162-1170

[14]Ruiz-Sanchez E, Lange A B, Orchard I. 2010b. Effects of themycotoxin destruxin A on Locusta migratoria visceralmuscles. Toxicon, 56, 1043-1051

[15]Sowjanya Sree K, Padmaja V. 2008. Oxidative stress inducedby destruxin from Metarhizium anisopliae (Metch.)involves changes in glutathione and ascorbatemetabolism and instigates ultrastructural changes inthe salivary glands of Spodoptera litura (Fab.) larvae.Toxicon, 51, 1140-1150

[16]Vazquez M J, Albarran M I, Espada A, Rivera-Sagredo A,Diez E, Hueso-Rodriguez J A. 2005. A new destruxin asinhibitor of vacuolar-type H+-ATPase of Saccharomycescerevisiae. Chemistry & Biodiversity, 2, 123-130

[17]Vilcinskas A, Matha V, Gotz P. 1997a. Effects of theentomopathogenic fungus Metarhizium anisopliaeand its secondary metabolites on morphology andcytoskeleton of plasmatocytes isolated from the greaterwax moth Galleria mellonella. Journal of InsectPhysiology, 43, 1149-1159

[18]Vilcinskas A, Matha V, Gotz P. 1997b. Inhibition ofphagocytic activity of plasmatocytes isolated fromGalleria mellonella by entomogenous fungi and theirsecondary metabolites. Journal of Insect Physiology,43, 475-483

[19]Vey A, Matha V, Dumas C. 2002. Effects of the peptidemycotoxin destruxin E on insect haemocytes and ondynamics and efficiency of the multicellular immunereaction. Journal of Invertebrate Pathology, 80, 177-187

[20]Wang B, Kang Q, Lu Y, Bai, L, Wang C. 2012. Unveiling thebiosynthetic puzzle of destruxins in Metarhiziumspecies. Proceedings of the National Academy ofSciences of the United States of America, 109, 1287-1292

[21]Yukiko S, Yushi F, Etsu T, Masaya I. 2009. Vacuolar H+-ATPase inhibitors overcome Bcl-xL-mediatedchemoresistance through restoration of a caspaseindependentapoptotic pathway. Cancer Science, 100,1460-1467.
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