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Journal of Integrative Agriculture  2024, Vol. 23 Issue (2): 484-493    DOI: 10.1016/j.jia.2023.05.039
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Creating large EMS populations for functional genomics and breeding in wheat

Wenqiang Wang1, 2*, Xizhen Guan1*, Yong Gan3, Guojun Liu3, Chunhao Zou1, Weikang Wang1, Jifa Zhang1, Huifei Zhang1, Qunqun Hao2, Fei Ni3, Jiajie Wu3, Lynn Epstein4, Daolin Fu1, 3#

1 Spring Valley Agriscience Co., Ltd., Jinan 250300, China

2 College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China

3 National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai’an 271018, China

4 Department of Plant Pathology, University of California, Davis, CA 95616, USA

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摘要  

小麦种质资源是基础研究、应用研究和小麦遗传育种的基础资源,可以通过收集野生资源、积累育种品系、引入诱变材料等多种途径获得。甲基磺酸乙酯(EMS)是一种烷化剂,在植物中可以有效的诱导产生遗传变异。本研究中,我们以中国的小麦品种鲁研128”济麦38”济麦44”山农30”亲本,创制了包含百万级的EMS突变群体。在M1代中,突变群体的表型变异丰富,鉴定到>3000个叶绿素缺失突变体、2519个密穗突变体和1692个雄性不育突变体。此外,我们也鉴定到一些稀有突变,例如30个双穗突变。遗传学分析发现M1代中部分叶绿素缺失和密穗突变体在M2M3都能稳定遗传。为了更好的保留该突变群体达到更高的世代,我们设计了单粒传的保存方法。目前,我们正在用M2突变群体筛选其它重要的农艺性状,如抗精喹禾灵除草剂的突变体。本突变群体可用于科研合作,旨在为小麦的基础研究和遗传育种提供一个有价值的工具。



Abstract  

Wheat germplasm is a fundamental resource for basic research, applied studies, and wheat breeding, which can be enriched normally by several paths, such as collecting natural lines, accumulating breeding lines, and introducing mutagenesis materials.  Ethyl methane sulfonate (EMS) is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species.  In this study, we created a million-scale EMS population (MEP) that started with the Chinese wheat cultivars ‘Luyan 128’, ‘Jimai 38’, ‘Jimai 44’, and ‘Shannong 30’.  In the M1 generation, the MEP had numerous phenotypical variations, such as >3,000 chlorophyll-deficient mutants, 2,519 compact spikes, and 1,692 male sterile spikes.  There were also rare mutations, including 30 independent tillers each with double heads.  Some M1 variations of chlorophyll-deficiency and compact spikes were inheritable, appearing in the M2 or M3 generations.  To advance the entire MEP to higher generations, we adopted a single-seed descendent (SSD) approach.  All other seed composites of M2 were used to screen other agronomically important traits, such as the tolerance to herbicide quizalofop-P-methyl.  The MEP is available for collaborative projects, and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture.

Keywords:  wheat        germplasm        ethyl methane sulfonate        genetics and breeding   
Received: 31 January 2023   Accepted: 15 May 2023
Fund: 

This work was supported by the National Key Research and Development Program of China (2022YFF1002300), the Quancheng ‘5150’ Talent Program, China (07962021047) and the Agriculture Applied Technology Initiative of Jinan Government, China (CX202113).


About author:  Wenqiang Wang, E-mail: wangwenqiang881202@163.com; Xizhen Guan, E-mail: xzhguan@svjn.com.cn; #Correspondence Daolin Fu, Tel: +86-531-88766999, E-mail: dlfu@svjn.com.cn, dlfu@sdau.edu.cn

Cite this article: 

Wenqiang Wang, Xizhen Guan, Yong Gan, Guojun Liu, Chunhao Zou, Weikang Wang, Jifa Zhang, Huifei Zhang, Qunqun Hao, Fei Ni, Jiajie Wu, Lynn Epstein, Daolin Fu. 2024.

Creating large EMS populations for functional genomics and breeding in wheat . Journal of Integrative Agriculture, 23(2): 484-493.

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