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Journal of Integrative Agriculture  2024, Vol. 23 Issue (2): 494-510    DOI: 10.1016/j.jia.2023.04.029
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Metabolic profiles and morphological characteristics of leaf tips among different sweet potato (Ipomoea batatas Lam.) varieties

Wenqing Tan1, Xinbo Guo2, Zhangying Wang1, Rong Zhang1, Chaochen Tang1, Bingzhi Jiang1, Ruixue Jia1, Yuanyuan Deng3, Shaohai Yang1#, Jingyi Chen1#

1 Crops Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement of Guangdong Province, Guangzhou 510640, China

2 School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China

3 Sericultural & Agri-Food Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs/Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, China

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摘要  

甘薯茎尖具有很高的营养价值,探究不同甘薯品种茎尖代谢物特征的差异可以为提高其品质提供参考信息。在本研究中,采用基于UPLC-Q-Exactive Orbitrap/MS的非靶向代谢组学方法对32个甘薯品种茎尖中代谢产物进行了鉴定。其中,鉴定出了三个品种A01A02A03具有特异的总体代谢物特征,两个品种A20A18具有特异的酚酸类物质代谢特征,以及三个品种A05A12A16具有特异的黄酮类物质代谢特征。此外,通过形态学表征,共鉴定出163个和29个与甘薯茎尖颜色和叶片形状相关的差异代谢物。通过表型性状的分组比较分析和代谢物-表型性状的相关性分析表明,甘薯茎尖的颜色差异与黄酮类化合物紧密相关。同时,也发现多酚类物质含量与甘薯茎尖的叶片状相关,缺刻类型比全缘类型含有较多较高含量的多酚类物质。以上关于不同甘薯茎尖代谢物特征分析的结果以及鉴定到的与甘薯茎尖形态相关的差异代谢物将为培育茎尖具有更高营养价值的甘薯品种提供有用的信息。



Abstract  

Sweet potato leaf tips have high nutritional value, and exploring the differences in the metabolic profiles of leaf tips among different sweet potato varieties can provide information to improve their qualities.  In this study, a UPLC-Q-Exactive Orbitrap/MS-based untargeted metabolomics method was used to evaluate the metabolites in leaf tips of 32 sweet potato varieties.  Three varieties with distinct overall metabolic profiles (A01, A02, and A03), two varieties with distinct profiles of phenolic acids (A20 and A18), and three varieties with distinct profiles of flavonoids (A05, A12, and A16) were identified.  In addition, a total of 163 and 29 differentially expressed metabolites correlated with the color and leaf shape of sweet potato leaf tips, respectively, were identified through morphological characterization.  Group comparison analysis of the phenotypic traits and a metabolite-phenotypic trait correlation analysis indicated that the color differences of sweet potato leaf tips were markedly associated with flavonoids.  Also, the level of polyphenols was correlated with the leaf shape of sweet potato leaf tips, with lobed leaf types having higher levels of polyphenols than the entire leaf types.  The findings on the metabolic profiles and differentially expressed metabolites associated with the morphology of sweet potato leaf tips can provide useful information for breeding sweet potato varieties with higher nutritional value.

Keywords:  sweet potato        leaf tips        phenotypic traits        metabolic profile        differentially expressed metabolites        polyphenols   
Received: 06 January 2023   Accepted: 10 March 2023
Fund: 

This work was supported by grants from the construction and operation of the Food Nutrition and Health Research Center of Guangdong Academy of Agricultural Sciences, China (XTXM 202205), the earmarked fund for CARS-10-Sweetpotato, and the Guangdong Modern Agro-industry Technology Research System, China (2022KJ111).  

About author:  Wenqing Tan, E-mail: twq8391290618@163.com; #Correspondence Shaohai Yang, E-mail: yangshaohai@gdaas.cn; Jingyi Chen, Tel/Fax: +86-10-87593931, E-mail: victerchen@163.com

Cite this article: 

Wenqing Tan, Xinbo Guo, Zhangying Wang, Rong Zhang, Chaochen Tang, Bingzhi Jiang, Ruixue Jia, Yuanyuan Deng, Shaohai Yang, Jingyi Chen. 2024.

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