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Investigation of Aegilops umbellulata for stripe rust resistance, heading date, and the contents of iron, zinc, and gluten protein
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| SONG Zhong-ping1, 2, ZUO Yuan-yuan2, XIANG Qin2, LI Wen-jia2, LI Jian2, LIU Gang2, DAI Shou-fen2, YAN Ze-hong1, 2# |
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, P.R.China
2 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
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摘要
小伞山羊草二倍体 (Ae. umbellulata, 2n=2x=14, UU) 是对普通小麦遗传改良具有潜在利用价值的小麦近缘植物。本研究报道了46份小伞山羊草的条锈病抗性、抽穗期、微量元素铁、锌含量以及“面筋蛋白”含量的调查结果。在四个环境下，42份小伞山羊草表现抗小麦条锈病，4份感条锈病。小伞山羊草的平均抽穗期 (180.9天) 显著晚于3个普通小麦对照 (137.0天)，但1份材料PI226500除外，为138.9天。小伞山羊草材料间的铁、锌含量有广泛的变异，变幅分别为69.74–348.09 mg/Kg和49.83–101.65 mg/Kg。PI 542362, PI 542363和PI 5543993份这3份材料的铁、锌含量高于其他小伞山羊草，分别为230.96–348.09 mg/Kg和92.46–101.65 mg/Kg。小伞山羊草的铁含量与顶芒山羊草 (Ae. comosa, 2n = 2x =14, MM) 和尾状山羊草 (Ae. markgrafii, 2n = 2x =14, CC) 的相当，但高于节节麦 (Ae. tauschii, 2n = 2x =14, DD) 和普通小麦对照。小伞山羊草的锌含量高于节节麦、顶芒山羊草和普通小麦对照，但低于尾状山羊草。用高效液相色谱分析了小伞山羊草以及作为对照的其他山羊草二倍体的面筋蛋白含量。与其他物种相比较，小伞山羊草具有独特的洗脱峰，如41-42分钟的低分子量谷蛋白以及大约57分钟时的γ-醇溶蛋白。在所研究的物种中，小伞山羊草的γ-醇溶蛋白含量是最高的 (小伞山羊草vs. 其他物种，平均含量: 72.11%% vs. 49.37%，变异幅度 55.33–86.99% vs. 29.60–67.91%)。这些研究结果表明，小山羊草在这些性状上有很大遗传变异，是可供用于普通小麦相关性状遗传改良利用的潜在基因库。
Abstract
Aegilops umbellulata (UU) is a wheat wild relative that has potential use in the genetic improvement of wheat. In this study, 46 Ae. umbellulata accessions were investigated for stripe rust resistance, heading date (HD), and the contents of iron (Fe), zinc (Zn), and seed gluten proteins. Forty-two of the accessions were classified as resistant to stripe rust, while the other four accessions were classified as susceptible to stripe rust in four environments. The average HD of Ae. umbellulata was significantly longer than that of three common wheat cultivars (180.9 d vs. 137.0 d), with the exception of PI226500 (138.9 d). The Ae. umbellulata accessions also showed high variability in Fe (69.74–348.09 mg kg–1) and Zn (49.83–101.65 mg kg–1) contents. Three accessions (viz., PI542362, PI542363, and PI554399) showed relatively higher Fe (230.96–348.09 mg kg–1) and Zn (92.46–101.65 mg kg–1) contents than the others. The Fe content of Ae. umbellulata was similar to those of Ae. comosa and Ae. markgrafii but higher than those of Ae. tauschii and common wheat. Aegilops umbellulata showed a higher Zn content than Ae. tauschii, Ae. comosa, and common wheat, but a lower content than Ae. markgrafii. Furthermore, Ae. umbellulata had the highest proportion of γ-gliadin among all the species investigated (Ae. umbellulata vs. other species=mean 72.11% vs. 49.37%; range: 55.33–86.99% vs. 29.60–67.91%). These results demonstrated that Ae. umbellulata exhibits great diversity in the investigated traits, so it can provide a potential gene pool for the genetic improvement of these traits in wheat.
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Received: 07 January 2022
Accepted: 16 March 2022
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This work was supported by the National Natural Science Foundation of China (31771783), the Key Research and Development Program of Sichuan Province, China (2021YFYZ0002), and the Sichuan Science and Technology Program, China (2018HH0130 and 2022YFH0105).
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| About author: SONG Zhong-ping, E-mail: 412559033@qq.com; #Correspondence YAN Ze-hong, Tel: +86-28-82650350, E-mail: zhyan104@163.com |
Cite this article:
SONG Zhong-ping, ZUO Yuan-yuan, XIANG Qin, LI Wen-jia, LI Jian, LIU Gang, DAI Shou-fen, YAN Ze-hong.
2023.
Investigation of Aegilops umbellulata for stripe rust resistance, heading date, and the contents of iron, zinc, and gluten protein
. Journal of Integrative Agriculture, 22(4): 1258-1265.
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