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| QTL analysis of the developmental changes in cell wall components and forage digestibility in maize (Zea mays L.) |
| LI Kun1, 2*, YANG Xue1, 2*, LIU Xiao-gang1, 2, HU Xiao-jiao1, 2, WU Yu-jin1, 2, WANG Qi1, 2, MA Fei-qian1, 2, LI Shu-qiang1, WANG Hong-wu1, 2, LIU Zhi-fang1, 2, HUANG Chang-ling1, 2 |
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 The National Engineering Laboratory for Crop Molecular Breeding, Beijing 10081, P.R.China
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摘要
细胞壁在保持茎秆强度和影响消化率方面起着重要的作用。木质素、纤维素、半纤维素是植物细胞壁的三大主要成分，均能通过影响细胞壁的结构和强度影响茎秆的品质。为了探索玉米茎秆次生细胞壁的动态发育过程的遗传机制。我们利用传统的QTL定位和条件QTL定位对玉米吐丝期后多个时期的细胞壁组分及消化率性状进行分析。通过对重组自交系群体中木质素、纤维素、酸性洗涤纤维、中性洗涤纤维、体外干物质消化率进行测量发现，在开花后的10-40天木质素、纤维素、酸性洗涤纤维、中性洗涤纤维呈现先增加后减少的趋势。体外干物质消化率与之相反。通过QTL定位共检测到72个与目标性状相关的QTL，单个位点可以解释3.48-24.04%的表型变异。其中部分QTL形成了6个热点区域，分别位于玉米染色体1.08、2.04、2.07、7.03、8.05、9.03bin上。1.08bin上的热点区域既控制细胞壁组分含量也与茎秆强度相关，在这个QTL区间内包含6个与细胞壁合成相关的基因。此外，利用多个时期的性状进行条件QTL定位分析检测到26个条件QTL，其中22个都发现于30天|20天和50天|40天。上述结果表明在玉米吐丝后的发育时期细胞壁组分相关性状受多个基因调控。通过聚合多个位点有利等位基因将有助于实现消化率性状和抗倒伏性状的协同改良。
Abstract Cell wall architecture plays a key role in stalk strength and forage digestibility. Lignin, cellulose, and hemicellulose are the three main components of plant cell walls, and they can impact stalk quality by affecting the structure and strength of the cell wall. To explore cell wall development during secondary cell wall lignification in maize stalks, conventional and conditional genetic mapping were used to identify the dynamic quantitative trait loci (QTLs) of the cell wall components and digestibility traits during five growth stages after silking. Acid detergent lignin (ADL), cellulose (CEL), acid detergent fiber (ADF), neutral detergent fiber (NDF), and in vitro dry matter digestibility (IVDMD) were evaluated in a maize recombinant inbred line (RIL) population. ADL, CEL, ADF, and NDF gradually increased from 10 to 40 days after silking (DAS), and then they decreased. IVDMD initially decreased until 40 DAS, and then it increased slightly. Seventy-two QTLs were identified for the five traits, and each accounted for 3.48–24.04% of the phenotypic variation. Six QTL hotspots were found, and they were localized in the 1.08, 2.04, 2.07, 7.03, 8.05, and 9.03 bins of the maize genome. Within the interval of the pleiotropic QTL identified in bin 1.08 of the maize genome, six genes associated with cell wall component biosynthesis were identified as potential candidate genes for stalk strength as well as cell wall-related traits. In addition, 26 conditional QTLs were detected in the five stages for all of the investigated traits. Twenty-two of the 26 conditional QTLs were found at 30 DAS conditioned using the values of 20 DAS, and at 50 DAS conditioned using the values of 40 DAS. These results indicated that cell wall-related traits are regulated by many genes, which are specifically expressed at different stages after silking. Simultaneous improvements in both forage digestibility and lodging resistance could be achieved by pyramiding multiple beneficial QTL alleles identified in this study.
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Received: 23 June 2021
Accepted: 08 September 2021
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Fund: This research was supported by the National Natural Science Foundation of China (31801367), the National Key Research and Development Program of China (2016YFD0101200) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
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| About author: LI Kun, E-mail: likun01@caas.cn; YANG Xue, E-mail: 1553802840@qq.com; Correspondence HUANG Chang-ling, Tel/Fax: +86-10-82108738, E-mail: huangchangling@caas.cn; WANG Hong-wu, Tel: +86-10-82108582, E-mail: wanghongwu@caas.cn; LIU Zhi-fang, Tel: +86-10-82108629, E-mail: liuzhifang@caas.cn
* These authors contributed equally to this study. |
Cite this article:
LI Kun, YANG Xue, LIU Xiao-gang, HU Xiao-jiao, WU Yu-jin, WANG Qi, MA Fei-qian, LI Shu-qiang, WANG Hong-wu, LIU Zhi-fang, HUANG Chang-ling.
2022.
QTL analysis of the developmental changes in cell wall components and forage digestibility in maize (Zea mays L.). Journal of Integrative Agriculture, 21(12): 3501-3513.
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