|
Special Issue:
动物科学Animal Science
|
|
|
|
| The tissue expression level of BPI gene in piglets from newborn to weaning and its relationship with Gram-negative bacterial infection |
DAI Chao-hui1, CAO Yue1, GAO Zhong-cheng1, ZHU Guo-qiang2, 3, WU Sheng-long1, 2, BAO Wen-bin1, 2 |
1 Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, P.R.China
2 Joint International Research Laboratory of Agriculture and Agri-Product Safety, Ministry of Education/Yangzhou University, Yangzhou 225009, P.R.China
3 College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, P.R.China |
|
|
|
|
Abstract The bactericidal/permeability increasing protein (BPI) has an important function of nonspecific killing of Gram-negative bacteria. In this study, qPCR was used to detect the expression of the BPI gene in twelve tissues of Meishan piglets from birth to weaning. BPI gene overexpression, bacterial adhesions count and indirect immunofluorescence were applied to analyze the relationship between BPI gene expression and the infectivity of Escherichia coli and Salmonella. The results showed that the BPI gene was expressed highly in duodenum, jejunum and ileum (fold changes of relative expression levels were more than 10 000, 500 and 200, respectively). The expression of the BPI gene at 35 days of age was significantly higher (P<0.01) than that at all other days. Transcription of the BPI gene was up-regulated 2 401-fold in porcine intestinal epithelial (IPEC-J2) cells that were transfected with the BPI gene overexpression lentivirus (IPEC-J2-BPI), and significantly higher (P<0.01) than that in negative control cells (IPEC-J2-NC). Protein expression levels in IPEC-J2-BPI cells were also increased. When IPEC-J2 cells were incubated with E. coli and Salmonella, respectively, for 2, 4, 6, 8, 10 and 12 h, the number of bacterial adhesions in IPEC-J2-BPI cells was significantly less (P<0.05) than that in IPEC-J2-NC cells. The results of indirect immunofluorescence analysis showed that the number of bacterial adhesions in IPEC-J2-BPI cells was significantly less (P<0.01) than that in IPEC-J2-NC cells. These results demonstrated that the BPI gene might play an important role in regulating weaning stress especially intestinal-mediated immune response. Overexpression of the BPI gene at the cellular level could significantly enhance the anti-bactericidal ability against Gram-negative bacteria such as E. coli and Salmonella. This has important biological significance in piglet resistance to bacterial diarrhea.
|
|
Received: 16 September 2019
Accepted:
|
| Fund: This study was supported by the National Natural Science Foundation of China (31772560), the Yangzhou University International Academic Exchange Fund (YZUIAEF201901005), the College Students’ Innovation and Entrepreneurship Training Program of Jiangsu Province, China (201811117014Z), and the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions. |
|
Corresponding Authors:
Correspondence BAO Wen-bin, E-mail: wbbao@yzu.edu.cn
|
| About author: DAI Chao-hui, E-mail: dx120170081@yzu.edu.cn; |
Cite this article:
DAI Chao-hui, CAO Yue, GAO Zhong-cheng, ZHU Guo-qiang, WU Sheng-long, BAO Wen-bin.
2020.
The tissue expression level of BPI gene in piglets from newborn to weaning and its relationship with Gram-negative bacterial infection. Journal of Integrative Agriculture, 19(12): 3065-3073.
|
Baron O L, van West P, Industri B, Ponchet M, Dubreuil G, Gourbal B, Reichhart J M, Coustau C. 2013. Parental transfer of the antimicrobial protein LBP/BPI protects Biomphalaria glabrata eggs against oomycete infections. PLoS Pathogens, 9, e1003792.
Calafat J, Janssen H, Tool A, Dentener M A, Knol E F, Rosenberg H F, Egesten, A. 1998. The bactericidal/permeability-increasing protein (BPI) is present in specific granules of human eosinophils. Blood, 91, 4770–4775.
Dai C, Dong W, Wu J, Bao W, Wu S. 2016. The function of pig bactericidal permeability protein (BPI) and its research progress in resistance breeding. China Animal Husbandry & Veterinary Medicine, 43, 2736–2741. (in Chinese)
Dentener M A, Francot G J, Buurman W A. 1996. Bactericidal/permeability-increasing protein, a lipopolysaccharide-specific protein on the surface of human peripheral blood monocytes. Journal of Infectious Diseases, 173, 252–255.
Domingues M M, Silva P M, Franquelim H G, Carvalho F A, Castanho M A, Santos N C. 2014. Antimicrobial protein rBPI21-induced surface changes on Gram-negative and Gram-positive bacteria. Nanomedicine, 10, 543–551.
Elsbach P, Weiss J. 1993. Bactericidal/permeability increasing protein and host defense against Gram-negative bacteria and endotoxin. Current Opinion in Immunology, 5, 103–107.
Gao H, Yuan J, Xiao G. 2005. Protective effects of bactericidal/permeability increasing protein simulated peptide on murine acute lung injury induced by lipopolysaccharide. Chinese Journal of Burns, 21, 100–103. (in Chinese)
Holweg A, Schnare M, Gessner A. 2011. The bactericidal/permeability-increasing protein (BPI) in the innate defence of the lower airways. Biochemical Society Transactions, 39, 1045–1050.
Jung W C, Cha C N, Lee Y E, Yoo C Y, Park E K, Kim S, Lee H J. 2011. Anti-diarrheal effects of a combination of Korean traditional herbal extracts and dioctahedral smectite on piglet diarrhea caused by Escherichia coli and Salmonella typhimurium. Pakistan Veterinary Journal, 31, 336–340.
Kreuzer S, Reissmann M, Brockmann G A. 2013. New fast and cost-effective gene test to get the ETEC F18 receptor status in pigs. Veterinary Microbiology, 163, 392–394.
Liu L, Wang J, Zhao Q, Zi C, Wu Z, Su X, Huo Y, Zhu G, Wu S, Bao W. 2013. Genetic variation in exon 10 of the BPI gene is associated with Escherichia coli F18 susceptibility in Sutai piglets. Gene, 523, 70–75.
Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods, 25, 402–408.
Pinkenburg O, Meyer T, Bannert N, Norley S, Bolte K, Czudai-Matwich V, Herold S, Gessner A, Schnare M. 2016. The human antimicrobial protein bactericidal/permeability-increasing protein (BPI) inhibits the infectivity of influenza a virus. PLoS ONE, 11, e0156929.
Qin W Y, Gan L N, Xia R W, Sun S Y, Zhu G Q, Wu S L, Bao W B. 2017. New insights into the codon usage patterns of the bactericidal/permeability-increasing (BPI) gene across nine species. Gene, 616, 45–51.
Ruiz V L, Bersano J G, Carvalho A F, Catroxo M H, Chiebao D P, Gregori F, Miyashiro S, Nassar A F, Oliveira T M, Ogata R A, Scarcelli E P, Tonietti P O. 2016. Case-control study of pathogens involved in piglet diarrhea. BMC Mesearch Notes, 9, 22.
van der Schaft D W J, Wagstaff J, Mayo K H, Griffioen A W. 2002. The antiangiogenic properties of bactericidal/permeability-increasing protein (BPI). Annals of Medicine, 34, 19–27.
Sun L, Wang J, Yin X, Zi C, Zhu G, Wu S, Bao W. 2016. Identification of a 5-methylcytosine site that may regulate C/EBPβ binding and determine tissue-specific expression of the BPI gene in piglets. Scientific Reports, 6, 28506.
Tobias P S, Soldau K, Iovine N M, Elsbach P, Weiss J. 1997. Lipopolysaccharide (LPS)-binding proteins BPI and LBP from different types of complexes with LPS. Journal of Biological Chemistry, 272, 18682–18685.
Wiesner J, Vilcinskas A, 2010. Antimicrobial peptides: The ancient arm of the human immune system. Virulence, 1, 440–464.
Wittmann I, Schönefeld M, Aichele D, Groer G, Gessner A, Schnare M. 2008. Murine actericidal/permeability-increasing protein inhibits the endotoxic activity of lipopolysaccharide and Gram-negative bacteria. Journal of Immunology, 180, 7548–7552.
Wu Z, Su X, Wang J, Zheng X, Wu S, Bao W. 2013a. Bioinformatics analysis on the coding region of BPI gene among thirteen different species. China Animal Husbandry & Veterinary Medicine, 40, 28–32. (in Chinese)
Wu Z, Wang J, Zhu S, Zhao Q, Liu L, Zi C, Wu S, Bao W. 2013b. The partial cSNPs detection of pig BPI gene and its infection on protein structures and function. Chinese Journal of Animal and Veterinary Sciences, 44, 1000–1007. (in Chinese)
Ye L, Zi C, Liu L, Zhu J, Xie K, Zhu G, Huang X G, Bao W B, Wu S L, Wang J Y. 2011. Study on the relationship between the expression of BPI gene and Escherichia coli F18 infection in piglets. Hereditas (Beijing), 33, 1225–1230. (in Chinese)
Zhao M H, Jones S J, Lockwood C M. 1995. Bactericidal/permeability-increasing protein (BPI) is an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis. Clinical and Experimental Immunology, 99, 49–56.
Zhu J, Zi C, Wu Z C, Liu L, Zheng X R, Su X M, Zhu G Q, Huang X G, Wu S L, Bao W B. 2013. Age-dependent expression of the BPI gene in Sutai piglets. Genetics and Molecular Research (GMR), 12, 2120–2126.
|
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
