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Journal of Integrative Agriculture  2020, Vol. 19 Issue (1): 251-264    DOI: 10.1016/S2095-3119(18)62122-7
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation
LIN Wei-peng1, 2, 3, JIANG Ni-hao1, 3, PENG Li4, FAN Xue-ying1, 3, GAO Yang1, 3, WANG Guo-ping5, CAI Kun-zheng1, 3  #br#
1 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China
2 Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, P.R.China
3 Key Laboratory of Tropical Agricultural Environment in South China, Ministry of Agriculture, Guangzhou 510642, P.R.China
4 College of Agriculture, South China Agricultural University, Guangzhou 510642, P.R.China
5 College of Horticulture, South China Agricultural University, Guangzhou 510642, P.R.China
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Silicon (Si) can increase plant resistance against bacterial wilt caused by Ralstonia solanacearum and enhance plant immune response.  However, whether Si alleviates soil-borne disease stress through altering soil microbial community component and diversity is not clear.  In this study, effects of Si application under R. solanacearum inoculation with or without plant on soil bacterial and fungal communities were investigated through high-throughput pyrosequencing technique.  The results showed that Si addition significantly reduced bacterial wilt incidence.  However, Si did not reduce the amount of R. solanacearum in rhizosphere soil.  Principal components analysis showed that soil microbial community composition was strongly influenced by Si addition.  Total 63.7% bacterial operational taxonomic units (OTUs) and 43.8% fungal OTUs were regulated by Si addition regardless of the presence of tomato plants, indicating the independent effects of Si on soil microbial community.  Si-added soil harbored a lower abundance of Fusarium, Pseudomonas, and Faecalibacterium.  Our finding further demonstrated that exogenous Si could significantly influence soil microbial community component, and this may provide additional insight into the mechanism of Si-enhanced plant resistance against soil-borne pathogens.
Keywords:  bacterial wilt        deep pyrosequencing        Ralstonia solanacearum        silicon        soil microbial community  
Received: 20 August 2018   Accepted:
Fund: This study was financially supported by grants from the National Natural Science Foundation of China (31370456), the Doctoral Foundation of the Ministry of Education of China (20124404110007), the Natural Science Foundation of Guangdong Province of China (S2012010010331 and 2017A030313177), and the Project of International, as well as Hong Kong, Macao & Taiwan Science and Technology Cooperation Innovation Platform in Universities in Guangdong Province, China (2014KGJHZ004).
Corresponding Authors:  Correspondence CAI Kun-zheng, Mobile: +86-13672408736, E-mail:   
About author:  LIN Wei-peng, Mobile: +86-13570532369, E-mail:;

Cite this article: 

LIN Wei-peng, JIANG Ni-hao, PENG Li, FAN Xue-ying, GAO Yang, WANG Guo-ping, CAI Kun-zheng . 2020. Silicon impacts on soil microflora under Ralstonia Solanacearum inoculation. Journal of Integrative Agriculture, 19(1): 251-264.

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