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Special Issue:
植物抗病遗传合辑Plant Disease-resistance Genetics
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| The TaFIM1 gene mediates wheat resistance against Puccinia striiformis f. sp. tritici and responds to abiotic stress |
SHI Bei-bei1, WANG Juan1, 2, GAO Hai-feng3, ZHANG Xiao-juan1, WANG Yang1, MA Qing1 |
1 State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling 712100, P.R.China
2 School of Life Science, Shanxi Datong University, Datong 037009, P.R.China
3 Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences/Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Urumqi 830091, P.R.China |
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摘要
丝束蛋白（fimbrin）是肌动蛋白细胞骨架的调节因子，参与并控制多种组织和细胞的生理生化和发育过程。然而，fimbrin在对病原菌防御中，特别是在小麦抗条锈病中的作用研究匮乏，其机制尚待阐明。本研究以小麦品种水源11（Suwon 11）与条锈菌（Puccinia striiformis f. sp. tritici，Pst）生理小种CYR23组成非亲和互作，与生理小种CYR31组成成亲和互作，利用实时荧光定量 PCR 技术（qRT-PCR）对TaFIM1基因参与小麦抗条锈病的功能进行初步分析；对在非生物胁迫和施用外源激素处理TaFIM1基因的表达特征进行分析；通过病毒诱导的基因沉默（BSMV-VIGS）技术，验证TaFIM1在小麦抗条锈病中的功能。获得以下研究结果：TaFIM1在非亲和互作中的表达量显著上调，且在48 h表达量达到峰值，是对照0 h的6.0倍；在亲和互作中，TaFIM1的表达量无明显变化。TaFIM1能够响应不同非生物胁迫，在高温（Hot）、低温（Cool）、盐（NaCl）和干旱（PEG6000）胁迫下诱导TaFIM1基因表达量上调。BSMV-VIGS试验结果显示，借助大麦条纹花叶病毒对TaFIM1基因进行诱导沉默。对沉默成功的小麦植株分别接种条锈菌CYR23和CYR31。在非亲和互作中，沉默植株的抗病性降低，叶片上出现少量的夏孢子堆；在亲和互作中，与对照相比，叶片上的夏孢子堆数量增加，沉默植株的感病性增强。组织学观察发现，在48 h和120 h，TaFIM1沉默植株叶片中菌丝分支数和菌丝长度高于对照，在120 h基因沉默植株叶片中菌落面积显著高于对照组，表明TaFIM1沉默后小麦植株与对照相比感病性增强，进一步说明TaFIM1参与植物的抗病性。因此，TaFIM1与植物抗病性相关，在小麦抵抗条锈病的侵染过程中响应正调控作用。本研究为理解fimbrin在小麦中的作用提供了新的见解。
Abstract Fimbrin, a regulator of actin cytoskeletal dynamics that participates in numerous physiological and biochemical processes, controls multiple developmental processes in a variety of tissues and cell types. However, the role of fimbrin in pathogen defense of wheat and the mechanisms have not been well studied. Here, we investigated that the expression of TaFIM1 gene of wheat was significantly induced in response to avirulent race of Puccinia striiformis f. sp. tritici (Pst) and silencing of TaFIM1 by virus-induced gene silencing method. The results show that silencing of TaFIM1 resulted in a reduction of resistance against the stripe rust indicated by both phenotypes and a histological examination of Pst growth. Additionally, the expression level of TaFIM1 gene was up-regulated under abiotic stresses. These findings suggest that TaFIM1 functions as a positive regulator of pathogen resistance of wheat plants and response to abiotic stress. Our work may show new light on understanding the roles of fimbrin in wheat.
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Received: 03 March 2020
Accepted:
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| Fund: The research was supported by the National Natural Science Foundation of China (31571960), the NSFC-Xinjiang Joint Fund, China (U1903110) and the 111 Project from the Ministry of Education of China (B07049). |
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Corresponding Authors:
Correspondence WANG Yang, E-mail: wangyang2006@nwsuaf.edu.cn; MA Qing, E-mail: maqing@nwsuaf.edu.cn
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| About author: SHI Bei-bei, E-mail: shibeibei@nwafu.edu.cn |
Cite this article:
SHI Bei-bei, WANG Juan, GAO Hai-feng, ZHANG Xiao-juan, WANG Yang, MA Qing.
2021.
The TaFIM1 gene mediates wheat resistance against Puccinia striiformis f. sp. tritici and responds to abiotic stress. Journal of Integrative Agriculture, 20(7): 1849-1857.
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