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| Characterization of T-complex polypeptide 1 (TCP-1) from the Chilo suppressalis HSP60 family and its expression in response to temperature stress |
| YU Tong-ying1, 2, LU Ming-xing2, CUI Ya-dong1 |
1 Biology and Food Engineering School, Fuyang Normal University, Fuyang 236000, P.R.China
2 School of Horticulture and Plant Protection & Institute of Applied Entomology, Yangzhou University, Yangzhou 225009, P.R.China |
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摘要 Received 20 July, 2017 Accepted 4 September, 2017
© 2018 CAAS. Publishing services by Elsevier B.V. All rights reserved.
doi:
Abstract Many proteins require assistance from molecular chaperones at various stages to attain correctly folded states and functional conformations during protein synthesis. In this study, the gene encoding T-complex polypeptide 1 (TCP-1), which belongs to the heat shock protein 60 (HSP60) family, was isolated and characterized from the rice stem borer, Chilo suppressalis, by RACE and qPCR, respectively. The full-length cDNA of Tcp-1 was 2 144 bp and encoded a 1 635-bp ORF; the deduced translational product contained 545 amino acids with 5´- and 3´-UTRs and an isoelectric point of 5.29. Cluster analysis confirmed that the deduced amino acid sequence shared high identity (60–99%) with TCP-1 from other insects. To investigate Tcp-1 expression in response to abiotic stress, qPCR was used to analyze expression levels of Tcp-1 mRNA in C. suppressalis larvae exposed to temperatures ranging from –11 to 43°C. With respect to heat shock, Tcp-1 expression was higher than the control after a 2-h exposure to 30 and 36°C and declined at 39 and 43°C. Difference in Tcp-1 expression was observed at temperatures ranging from –11 to 27°C. qPCR analyses revealed that Tcp-1 expression was the highest in hindgut tissue as compared to heads, epidermis, fat body, foregut, midgut, and malpighian tubules. Our results indicated that Tcp-1 expression was differentially expressed in C. suppressalis tissues, and was impacted by temperature stress.
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Received: 20 July 2017
Accepted:
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| Fund: This research was funded by the National Natural Science Foundation of China (31401733) and the Incubation Study Project of Science and Technology of Fuyang Normal University, China (2014KJFH02). |
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Corresponding Authors:
Correspondence CUI Ya-dong, Tel/Fax: +86-558-2596148, E-mail: fysyswx@163.com; LU Ming-xing, E-mail: lumx@yzu.edu.cn
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| About author: YU Tong-ying, E-mail: 15655823391@163.com; |
Cite this article:
YU Tong-ying, LU Ming-xing, CUI Ya-dong.
2018.
Characterization of T-complex polypeptide 1 (TCP-1) from the Chilo suppressalis HSP60 family and its expression in response to temperature stress. Journal of Integrative Agriculture, 17(05): 1032-1039.
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