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| Comparative proteomics analysis of maize (Zea mays) leaves infected by small brown planthopper (Laodelphax striatellus) |
| ZHAO Mei-ai1*, LEI Zhen1*, PEI Yu-he2, SHAO Xiao-yu1, GUO Xin-mei2, SONG Xi-yun2 |
1 Key Laboratory of Plant Biotechnology in University of Shandong Province/College of Life Sciences, Qingdao Agricultural University, Qingdao 266109, P.R.China
2 Key Laboratory of Qingdao Major Crop Germplasm Resource Innovation and Application/College of Agronomy, Qingdao Agricultural University, Qingdao 266109, P.R.China |
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Abstract Maize rough dwarf disease (MRDD) is a viral disease caused by brown planthopper infestation, and leads to great yield loss, especially in China. Comparative proteomics was performed using maize inbred line Zheng 58 and LN 287. MRDD pathogen was detected as rice black-streaked dwarf virus (RBSDV) by quantitative real time PCR (qRT-PCR) in Shandong Province, China. The modified trichloroacetic acid (TCA)/acetone method was used for soluble protein extraction from leaves. Two-dimensional electrophoresis (2-DE) analysis was performed on 24-cm long, pH 4-7 linear immobilized pH gradient (IPG) strips, and gels were stained with silver and coomassie brilliant blue. We identified 944 proteins expressed in RBSDV infected maize leaves by proteomics approaches. Among these, 44 protein spots that revealed a 1.5-fold difference in intensity were identified by mass spectrometry between mock-inoculated and RBSDV infected samples. Among these, 17 and 26 spots were up-regulated, and 27 and 18 spots were down-regulated in the virus infected samples of Zheng 58 and LN 287, respectively. Differential protein spots were analyzed by mass spectrometry identification, which could be divided into six categories. Furthermore, the expression of stress-related proteins was detected and confirmed by qRT-PCR. This study lays the foundation for further investigations, enabling the enhancement of MRDD resistance in maize.
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Received: 21 May 2017
Accepted:
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| Fund: This research was funded by the National Natural Science Foundation of China (31371636), the Modern Agricultural System of Shandong Province, China (SDAIT-01-022-01), the Key Research and Development Project of Shandong Province, China (2016GNC110018), and the Applied Basic Research Project of Qingdao, China (14-2-4-13-jch). |
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Corresponding Authors:
Correspondence SONG Xi-Yun, Tel: +86-532-86080002, Fax: +86-532-86080640, E-mail: songxy@qau.edu.cn
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| About author: ZHAO Mei-ai, E-mail: meiai2015@163.com; * These authors contributed equally to this study.
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Cite this article:
ZHAO Mei-ai, LEI Zhen, PEI Yu-he, SHAO Xiao-yu, GUO Xin-mei, SONG Xi-yun? .
2018.
Comparative proteomics analysis of maize (Zea mays) leaves infected by small brown planthopper (Laodelphax striatellus). Journal of Integrative Agriculture, 17(04): 796-805.
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| Afroz A, Ali G M, Mir A, Komastsu S. 2011. Application of proteomics to investigate stress-induced proteins for improvement in crop protection. Plant Cell Reports, 30, 745–763.Brown R L, Chen Z, Menkir A, Cleveland T E. 2006. Proteomics to identify resistance factors in corn - A review. Mycotoxin Research, 22, 22–26.Chen Y, Chen X, Wang H J, Bao Y Q, Zhang W. 2014. Examination of the leaf proteome during flooding stress and the induction of programmed cell death in maize. Proteome Science, 12, 33–51.Edwards R, Dixon D P, Walbot V. 2000. Plant glutathione S-transferases, enzymes with multiple functions in sickness and in health. Trends in Plant Science, 5, 193–198.Gammulla C, Pascovici D, Atwell B, Haynes P. 2010. Differential metabolic response of cultured rice (Oryza sativa L.) cells exposed to high- and low-temperature stress. Proteomics, 10, 3001–3019.Huang Y, Ma H Y, Huang W, Wang F, Xu Z S, Xiong A S. 2016. Comparative proteomic analysis provides novel insight into the interaction between resistant vs. susceptible tomato cultivars and TYLCV infection. BMC Plant Biology, 16, 162–182.Jia M A, Li Y Q, Lei L, Di D P, Miao H Q, Fan Z F. 2012. Alteration of gene expression profile in maize infected with a double-stranded RNA fijivirus associated with symptom development. Molecular Plant Pathology, 13, 251–262.Jin X N, Li W H, Hu D S, Xia S, Zhang X X, Zhang F F, Fu Z Y, Ding D, Liu Z H, Tang J H. 2015. Biological responses and proteomic changes in maize seedlings under nitrogen deficiency. plant molecular biology reporter, 33, 490–504.Jing M F, Ma H Y, Li H Y, Guo B D, Zhang X, Ye W W, Wang H N, Wang Q X, Wangle Y C. 2015. Differential regulation of defense-related proteins in soybean during compatible and incompatible interactions between Phytophthora sojae and soybean by comparative proteomic analysis. Plant Cell Report, 34, 1263–1280. Khanna-Chopra R. 2012. Leaf senescence and abiotic stresses share reactive oxygen species-mediated chloroplast degradation. Protoplasma, 249, 469–481.Kundu S, Chakraborty D, Kundu A, Pal A. 2013. Proteomics approach combined with biochemical attributes to elucidate compatible and incompatible plant-virus interactions between Vigna mungo and Mungbean Yellow Mosaic India Virus. Proteome Science, 11, 15–28.Li G Y, Wang F H. 2008. Research progress of plant vitamin C peroxiredoxin. Advances in Morden Biomedicine, 8, 1147–1149.Li K P, Xu C Z, Zhang J R. 2011. Proteome profile of maize (Zea Mays L.) leaf tissue at the flowering stage after long-term adjustment to rice black-streaked dwarf virus infection. Gene, 485, 106–113.Lin F, Xu J, Shi J, Li H, Li B. 2010. Molecular cloning and characterization of a novel glyoxalase I gene TaGLY 1 in wheat (Triticum aestivum L.). plant molecular biology reporter, 37, 729–735.Liu C, Weng J, Zhang D, Zhang Z, Yang X, Shi L Y. 2014. Genome-wide association study of resistance to rough dwarf disease in maize. European Journal of Plant Pathology, 139, 205–216.Liu T X, Zhang L, Yuan Z L, Hu X L, Lu M H, Wang W, Wang Y. 2013. Identification of proteins regulated by ABA in response to combined drought and heat stress in maize roots. Acta Physiologiae Plantarum, 35, 501–513.Luan J W, Wang F, Li Y J, Zhang B, Zhang J R. 2012. Mapping quantitative trait loci conferring resistance to rice black-streaked virus in maize (Zea mays L.). Theoretical and Applied Genetics, 125, 781-791.Minamikawa T, Toyooka K, Okamoto T, Hara-Nishimura I, Nishimura M. 2001. Degradation of ribulose-bisphosphate carboxylase by vacuolar enzymes of senescing French bean leaves, immunocytochemical and ultrastructural observations. Protoplasma, 218, 144–153.Mustafiz A, Singh A K, Pareek A, Sopory S K, Singla-Pareek S L. 2011. Genome-wide analysis of rice and Arabidopsis identifies two glyoxalase genes that are highly expressed in abiotic stress. Functional & Integrative Genomics, 11, 293–305.Ono Y, Wada S, Izumi M, Makino A, Ishida H. 2013. Evidence for contribution of autophagy to Rubisco degradation during leaf senescence in Arabidopsis thaliana. Plant Cell and Environment, 36, 1147–1159.Sangha J S, Chen Y H, Kaur J, Khan W, Abduljaleel Z, Alanazi M S. 2013. Proteome analysis of rice (Oryza sativa L.). mutants reveals differentially induced proteins during brown planthopper Nilaparvata Iugens infestation. International Journal of Molecular Sciences, 14, 3921–3945.Singla-Pareek S L, Yadav S K, Pareek A, Reddy M K, Sopory S K. 2006. Transgenic tobacco overexpressing glyoxalase pathway enzymes grow and set viable seeds in zinc-spiked soils. Plant Physiology, 140, 613–623.Slykhuis J T. 1976. Virus and virus-like diseases of cereal crops. Annual Review of Pathology, 110, 893–908. Tao Y F, Liu Q C, Xu M L. 2013. The research progress on maize rough dwarf disease. Maize Science, 21, 149-152. (in Chinese)Wang F, Qin G Z, Sui Z H, Wang Z H, Wang Z Y, Yu J L, Zhang J R. 2006. Improved method for assaying maize plant resistance to maize rough dwarf disease by artificial inoculation and real-time RT-PCR. European Journal of Plant Pathology, 116, 289–300.Wu L J, Han Z P, Wang S X, Wang X T, Sun A G. 2013a. Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus. Journal of Proteomics, 89, 124–140.Wu L J, Zu X F, Wang X T, Sun A G, Zhang J, Wang S X, Chen Y H. 2013b. Comparative proteomic analysis of the effects of salicylic acid and abscisic acid on maize (Zea mays L.) leaves. plant molecular biology reporter, 31, 507–516.Yadav S K, Singla-Pareek S L, Ray M, Reddy M K, Sopory S K. 2005. Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. Biochemical and Biophysical Research Communications, 337, 61–67.Yang H, Huang Y P, Zhi H J, Yu D Y. 2011. Proteomics-based analysis of novel genes involved in response toward soybean mosaic virus infection. plant molecular biology reporter, 38, 511–521. Zhang H M, Yang J, Chen J P, Adams M J. 2008. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Archives of Virology, 153, 1893–1898. |
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