|
|
|
|
|
| Effects of different LED light wavelengths on the resistance of tomato against Botrytis cinerea and the corresponding physiological mechanisms |
| XU Hui, FU Yan-nan, LI Tian-lai, WANG Rui |
Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China |
|
|
|
|
Abstract New types of light-emitting diode (LED) sources were applied to irradiate Botrytis cinerea mycelium and tomato leaves that were inoculated with B. cinerea to assess the effect of different LED light wavelengths on the infection of tomato with B. cinerea, to determine the optimum light wavelengths to control B. cinerea, and to explore the mechanism of LED influence on the development of gray mold. The results showed that purple light and blue light irradiation significantly inhibited the growth of B. cinerea mycelium, and the inhibition rates were 22.3 and 15.16%, respectively, and purple light exhibited a better inhibitory effect than blue light. The lesion development of B. cinerea on tomato leaves was significantly inhibited upon irradiation with red and purple light with inhibition rates of 32.08 and 36.74%, respectively. Irradiation with red light inhibited the oxidative burst of superoxide anion (O2-.) that was caused by infection with B. cinerea, and red light regulated the H2O2 content in the tomato leaf, which increased and rapidly returned to a lower level. In addition, red light irradiation improved the activity of superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) in tomato leaves. However, purple light irradiation did not make tomato leaves exhibit this kind of physiological response. Therefore, red light and purple light can suppress gray mold in tomatoes, but the disease suppression mechanisms of these two types of LED light are different. In general, red light suppresses gray mold primarily by regulating the tomato defense mechanism for disease, whereas the suppression of tomato gray mold by purple light can be partially explained by the photo-inhibition of B. cinerea.
|
|
Received: 21 April 2016
Accepted:
Online: 22 June 2016
|
| Fund: This work was supported by the National Natural Science Foundation of China (31000921) and the Scientific Research Foundation for Returned Overseas Chinese Scholars. |
|
Corresponding Authors:
WANG Rui, Tel: +86-24-88487143, E-mail: ruiwangsyau@126.com
|
| About author: XU Hui, Tel: +86-24-88487143, E-mail: xuhuiaa@126.com |
Cite this article:
XU Hui, FU Yan-nan, LI Tian-lai, WANG Rui.
2017.
Effects of different LED light wavelengths on the resistance of tomato against Botrytis cinerea and the corresponding physiological mechanisms. Journal of Integrative Agriculture, 16(01): 106-114.
|
| Baptista F J, Bailey B J, Meneses J F. 2012. Effect of nocturnal ventilation on the occurrence of Botrytis cinerea in Mediterranean unheated tomato greenhouses. Crop Protection, 32, 142–149.Beers R F, Sizer I W. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry, 195, 133–140.Begum M, Hocking A D, Miskelly D. 2009. Inactivation of food spoilage fungi by ultra violet (UVC) irradiation. International Journal of Food Microbiology, 129, 74–77.Ballare C L. 2014. Light regulation of plant defense. Annual Reviews of Plant Biology, 65, 335–363.Cakmak I, Marschner H. 1992. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves. Plant physiology, 98, 1222–1227.Chen Q, Liu S Q, Zhang Z K, Cui H R, Hao S Q, Liu Z L. 2009. Effect of different light emitting diode sources on tomato fruit quality during color-changed period. Transactions of the CSAE, 25, 156–161. (in Chinese)Dai T, Gupta A, Huang Y, Yin R, Murray C K, Vrahas M S, Sherwood M E, Tegos G P, Hamblin M R. 2013. Blue light rescues mice from potentially fatal Pseudomonas aeruginosa burn infection: efficacy, safety, and mechanism of action. Antimicrobial Agents and Chemotherapy, 57, 1238–1245.Elmnasser N, Guillou S, Leroi F, Orange N, Bakhrouf A, Federighi M. 2007. Pulsed-light system as a novel food decontamination technology: A review. Canadian Journal of Microbiology, 53, 813–821.Enwemeka C S, Williams D, Hollosi S, Yens D, Enwemeka S K. 2008. Visible 405 nm SLD light photo-destroys methicillin-resistant Staphylococcus aureus (MRSA) in vitro. Lasers in Surgery and Medicine, 40, 734–737.Giannopolitis C N, Ries S K. 1977. Superoxide dismutases II. Purification and quantitative relationship with water-soluble protein in seedlings. Plant Physiology, 59, 315–318.Hori Y, Nishidate K, Nishiyama M, Kanahama K. 2011. Flowering and expression of flowering-related genes under long-day conditions with light-emitting diodes. Planta, 243, 321–330.Imada K, Tanaka S, Ibaraki Y, Yoshimura K, Ito S. 2014. Antifungal effect of 405-nm light on Botrytis cinerea. Letters in Applied Microbiology, 59, 670–676.Islam S Z, Honda Y, Arase S. 1998. Light-induced resistance of broad bean against Botrytis cinerea. Journal of Phytopathology, 146, 479–485.Islam S Z, Honda Y, Arase S. 1999. Some characteristic of red light-induced substance(s) against Botrytis cinerea produced in broad bean leaflets. Journal of Phytopathology, 147, 65–70.Jana S, Choudhuri M A. 1982. Glycolate metabolism of three submersed aquatic angiosperms during ageing. Aquatic Botany, 12, 345–354.Khanama N N, Uenoa M, Kiharab J, Hondac Y, Arase S. 2005. Suppression of red light-induced resistance in broad beans to Botrytis cinerea by salicylic acid. Physiological and Molecular Plant Pathology, 66, 20–29.Kim K, Kook H S, Jang Y J, Lee W H, Seralathan K K, Chae J C, Lee K J. 2013. The effect of blue-light-emitting diodes on antioxidant properties and resistance to Botrytis cinerea in tomato. Plant Pathology & Microbiology, 4, 203–208.Kretschmer M, Hahn M. 2008. Fungicide resistance and genetic diversity of Botrytis cinerea isolates from a vineyard in Germany. Journal of Plant Diseases & Protection, 115, 214–219.Li B J, Zhu G R. 1998. Diagnosis and integrated control of the development of tomato gray mold. Plant Protection, 24, 18–20. (in Chinese)Li Q, Kubota C. 2009. Effect of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany, 67, 59–64.Li X, Yue H, Wang S, Huang L Q, Ma J, Guo L P. 2013. Research of different effects on activity of plant antioxidant enzymes. China Journal of Chinese Materia Medica, 38, 973–978. (in Chinese)Li Z G, Gong M. 2005. Improvement of measurement method of superoxide anion radical in plant. Acta Botanica Yunnanica, 27, 211–216. (in Chinese)Maclean M, MacGregor S J, Anderson J G, Woolsey G A, Coia J E, Hamilton K, Taggart I, Watson S B, Thakker B, Gettinby G. 2010. Environmental decontamination of a hospital isolation room using high-intensity narrow-spectrum light. Journal of Hospital Infection, 76, 247–251.Massa G D, Kim H H, Wheeler R M, Mitchell C A. 2008. Plant productivity in response to LED lighting. HortScience, 43, 1951–1956.Munner S, Kim E J, Park J S, Lee J H. 2014. Influence of green, red and blue light emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.). International Journal of Molecular Sciences, 15, 4657–4670.Murdoch L E, Mckenzie K, Maclean M, Macgregor S J, Anderson J G. 2013. Lethal effects of high-intensity violet 405-nm light on Saccharomyces cerevisiae, Candida albicans, and on dormant and germinating spores of Aspergillus niger. Fungal Biology, 117, 519–527.Nakajima M, Akutsu K. 2014. Virulence factors of Botrytis cinerea. Journal of General Plant Pathology, 80, 15–23.O’Neill T M, Shtienberg D, Elad Y. 1997. Effect of some host and microclimate factors on infection of tomato stems by Botrytis cinerea. Plant Disease, 81, 36–40Parada R Y, Wataru M, Ueno M, Kihara J, Arase S. 2014. Red-light-induced resistance to brown spot disease caused by bipolaris oryzae in rice. Journal of Phytopathology, 163, 1–8.Rahman M Z, Honda Y, Arase S. 2003. Red-light-induced resistance in broad bean (Vicia faba L.) to leaf spot disease caused by Alternaria tenuissima. Journal of Phytopathology, 151, 86–91.Rahman M Z, Khanam H, Ueno M, Kihara J, Honda Y, Arase S. 2010. Suppression by red light irradiation of Corynespora leaf spot of cucumber caused by Corynespora cassiicola. Journal of Phytopathology, 158, 378–381.Shirasawa H, Ueno M, Kihara J, Arase S. 2012. Protective effect of red light against blast disease caused by Magnaporthe oryzae in rice. Crop Protect, 39, 41–44.Tokuno A, Ibaraki Y, Ito S, Araki H, Yoshimura K, Osaki K. 2012. Disease suppression in greenhouse tomato by supplementary lighting with 405 nm LED. Environment Control in Biology, 50, 19–29.Vänninen I, Pinto D M, Nissinen A I, Johansen N S, Shipp L. 2010. In the light of new greenhouse technologies: 1. Plant-mediated effects of artificial lighting on arthropods and tritrophic interactions. Annals of Applied Biology, 157, 393–414.Wang H, Jiang Y P, Yu H J, Xia X J, Shi K, Zhou Y H, Yu J Q. 2010. Light quality affects incidence of powdery mildew, expression of defence-related genes and associated metabolism in cucumber plants. European Journal of Plant Pathology, 127, 125–135.Williamson B, Tudzynski B, Tudzynski P, Van Kan J A L. 2007. Botrytis cinerea: the cause of grey mould disease. Molecular Plant Pathology, 8, 561–580.Xue X, Zhang Q, Wu J S. 2013. Research of reactive oxygen species in plants and its application on stress tolerance. Biotechnology Bulletin, 36, 6–11. (in Chinese)Yeh N, Chung J P. 2009. High-brightness LEDs — Energy efficient lighting sources and their potential in indoor plant cultivation. Renewable and Sustainable Energy Reviews, 13, 2175–2180.Yin Y Q, Hu J B, Deng M J. 2007. Latest development of antioxidant system and responses to stress in plant leaves. Chinese Agricultural Science Bulletin, 23, 105–110. (in Chinese)Yuan H L. 2012. Effects of different supplemental light qualities from LED source at night on growth and nutritional quality in leaf lettuce (Lactua Sativa L.) and the resistance to root-knot nematode (Meloidogyne Spp.) in cucumber plants. MSc thesis, Zhejiang University, Hangzhou. pp. 37–39. (in Chinese)Zhao T M, Yu W G, Zhao L P, Dong Y L, Chen H G, Li Y C, Yang M L. 2011. Research progress in breeding of tomato resistance to Botrytis cinerea. Jiangsu Journal of Agricultural Science, 27, 1141–1147. (in Chinese) |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
