Please wait a minute...
Journal of Integrative Agriculture  2016, Vol. 15 Issue (12): 2712-2718    DOI: 10.1016/S2095-3119(15)61290-4
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Molecular identification of ω-secalin gene expression activity in a wheat 1B/1R translocation cultivar
CHAI Jian-fang, ZHANG Cui-mian, MA Xiu-ying, WANG Hai-bo
Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract      ω-Secalin was an important factor influencing processing quality of wheat 1BL/1RS translocations. On the basis of four ω-secalin gene sequences cloned from Lankao 906 (a wheat cultivar with 1BL/1RS translocation) with putative transcription activity, a pair of primers with suitable restriction endonucleases added at their 5´ ends were designed to amplify the mature protein-coding regions of the four genes. After digestion with restriction endonucleases, the amplified products were ligated into the prokaryotic expression vector pET30a(+). The prokaryotically expressed recombinant proteins and gliadin isolated from the Lankao 906 seed were analyzed on the same acid polyacrylamide gel electrophoresis. All four prokaryotically expressed secalin bands had corresponding seed-expressed gliadin bands. The four corresponding gliadin bands were confirmed to be the expression products of the four ω-secalin genes by liquid chromatography tandem mass spectrometry (LC-MS/MS). This conclusion was further confirmed because the four ω-secalin bands could be detected in all 14 wheat 1BL/1RS translocation cultivars used in the study, although there was some interference for the detection of one ω-secalin band from nearby wheat gliadin bands. The sequence information of ω-secalin genes with expression activity will be helpful for improving the processing quality of wheat with 1BL/1RS translocations by using RNA interference method to silence the expression of the ω-secalin genes.
Keywords:  Triticum aestivum        processing quality        storage proteins        prokaryotic expression        LC-MS/MS  
Received: 20 November 2015   Accepted:

This research was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).

Corresponding Authors:  CHAI Jian-fang, Tel: +86-311-87652130, E-mail:; WANG Hai-bo, Tel: +86-311-87652006, E-mail:   

Cite this article: 

CHAI Jian-fang, ZHANG Cui-mian, MA Xiu-ying, WANG Hai-bo. 2016. Molecular identification of ω-secalin gene expression activity in a wheat 1B/1R translocation cultivar. Journal of Integrative Agriculture, 15(12): 2712-2718.

Barbeau W E, Schwarzlaff S S, Uriyo M G, Johnson J M, Harris C H, Griffey C A. 2003. Origin and practical significance of the sticky dough factor in 1BL/1RS wheats. Journal of the Science of Food and Agriculture, 83, 29–38.

Burnett C J, Lorenz K J, Carver B F. 1995. Effect of the 1B/1R translocation in wheat on composition and properties of grain and flour. Euphytica, 86, 159–166.

Carver B F, Rayburn A L. 1994. Comparison of related wheat stocks possessing 1B or 1BL.1RS chromosomes: Agronomic performance. Crop Science, 34, 1505–1510.

Chai J F, Lu X, Jia J Z. 2005. Homoelogous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation. Cell Research, 15, 658–664.

Clarke B C, Appels R. 1999. Sequence variation at the Sec-1 locus of rye, Secale cereale (Poaceae). Plant Systemics and Evolution, 214, 1–14.

Clarke B C, Mukai Y, Apples R. 1996. The Sec-1 locus on the short arm of chromosome 1R of rye (Secale cereale). Chromosoma, 105, 269–275.

Dhaliwal A S, Mares D J, Marshall D R. 1987. Effect of 1B/1R chromosome translocation on milling and quality characteristics of bread wheats. Cereal Chemistry, 64, 72–76.

Dhaliwal A S, Mares D J, Marshall D R. 1990. Measurement of dough surface stickiness associated with the 1B/1R chromosome translocation in bread wheats. Cereal Science, 12, 165–175.

Dhaliwal A S, Mares D J, Marshall D R, Skerritt J H. 1988. Protein composition and pentosan content in relation to dough stickiness of 1B/1R translocation wheats. Cereal Chemistry, 65, 143–149.

Draper S R. 1987. ISTA variety committee - Report of the working group for biochemical tests for cultivar identification 1983–1986. Seed Science and Technology, 15, 431–434.

Graybosh R A, Peterson C J, Hansen L E, Mattern P J. 1990. Relationship between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition and end-use quality in winter wheat germplasm. Cereal Chemistry, 67, 342–349.

Guberac S, Petrovic S, Guberac V, Maric S. 2015. 1RS translocation in Croatian winter wheat varieties. Poljoprivreda/Agriculture, 21, 8–14.

Hull G A, Halford N G, Kreis M, Shewry P R. 1991. Isolation and characterization of genes encoding rye prolamins containing a highly repetitive sequence motif. Plant Molecular Biology, 17, 1111–1115.

Hussain A, Lukow O M. 1994. Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate. Euphytica, 78, 109–113.

Javornik B, Sinkovic T, Vapa L, Koebner R M D, Rogers W J. 1991. A comparison of methods for identifying and surveying the presence of 1BL.1RS translocations in bread wheat. Euphytica, 54, 45–53.

Jiang Q T, Wei Y M, Andre L, Lu Z X, Pu  Z E, Peng Y Y, Zheng Y L. 2010. Characterization of ω-secalin genes from rye, triticale, and a wheat 1BL/1RS translocation line. Journal of Applied Genetics, 51, 403–411.

Lee J H, Graybosch R A, Peterson C J. 1995. Quality and biochemical effects of a 1RS/1BL wheat-rye translocation in wheat. Theoretical and Applied Genetics, 90, 105–112.

Lei M P, Li G R, Liu C, Yang Z J. 2012. Characterization of wheat-Secale africanum introgression lines reveals evolutionary aspects of chromosome 1R in rye. Genome, 55, 765–774.

Li X F, Liu S B, Song Z Q, Wang H G. 2004. Development and molecular cytogenetic identification of 1RS·1BL translocation lines derived from Triticale×Tritileymus. Hereditas, 26, 481–485.

Liu J J, He Z H, Pena R J, Zhao Z D. 2004. The effects of 1B/1R translocation on grain quality and noodle quality of bread wheat. Acta Agronomic Sinica, 30, 149–153. (in Chinese)

Lookhart G L, Graybosch R, Peterson J, Lukaszewski A. 1991. Identification of wheat lines containing the 1BL/1RS translocation by high-performance liquid chromatography. Cereal Chemistry, 68, 312–316.

McKendry A L, Tague D N, Miskin K E. 1996. Effect of 1BL.1RS on agronomic performance of soft red winter wheat. Crop Science, 36, 844–847.

Moreno-Sevilla B, Baenziger P S, Peterson C J, Graybosch R A, McVey D V. 1995. The 1BL.1RS translocation: Agronomic performance of F3-derived lines from a winter wheat cross. Crop Science, 35, 1051–1055.

Schlegel R, Meinel A. 1994. A quantitative trait locus (QTL) on chromosome arm 1RS of rye and its effect on yield performance of hexaploid wheats. Cereal Research Communications, 22, 7–13.

Tahir R, Bux H, Kazi A G, Rasheed A, Napar A A, Ajmal S U, Mujeeb-Kazi A. 2014. Evaluation of Pakistani elite wheat germplasm for T1BL.1RS chromosome translocation. Journal of Agricultural Science and Technology, 16, 421–432.

Villarreal R L, Rajaram S, Mujeeb-Kazi A, Toro E. 1991. The effect of chromosome 1RS.1BL translocation on the yield potential of certain spring wheat (Triticum aestivum L.). Plant Breeding, 106, 77–81.

De Vita P, and Ficco D B M, Luciani A, Vincentini O, Pettoello-Mantovani M, Silano M, Maiuri L, Cattivelli L. 2012. A ω-secalin contained decamer shows a celiac disease prevention activity. Journal of Cereal Science, 55, 234–242.

Yamamoto M, Mukai Y. 2005. High-resolution physical mapping of the secalin-1 locus of rye on extended DNA fibers. Cytogenetic and Genome Research, 109, 79–82.

Yang M Y, Ren T H, Yan B J, Li Z, Ren Z L. 2014. Diversity resistance to Puccinia striiformis f. sp. Tritici in rye chromosome arm 1RS expressed in wheat. Genetics and Molecular Research, 13, 8783–8793.

Zhou J W, Han C X, Cao H, Zhen S M, Yu Z T, Li X H, Ma W J, Yan Y M. 2013. Fast identification of wheat 1BL.1RS translocation by reversed-phase ultra-performance liquid chromatography (RP-UPLC). Crop Pasture Science, 64, 865–873.

Zhou Y, He Z H, Zhang G S, Xia L Q, Chen X M, Gao Y C, Jing Z B, Yu G J. 2004. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agronomica Sinica, 30, 531–535. (in Chinese)
[1] CHI Qing, DU Lin-ying, MA Wen, NIU Ruo-yu, WU Bao-wei, GUO Li-jian, MA Meng, LIU Xiang-li, ZHAO Hui-xian. The miR164-TaNAC14 module regulates root development and abiotic-stress tolerance in wheat seedlings[J]. >Journal of Integrative Agriculture, 2023, 22(4): 981-998.
[2] ZHAO Ying-jia, ZHANG Yan-yang, BAI Xin-yang, LIN Rui-ze, SHI Gui-qing, DU Ping-ping, XIAO Kai. TaNF-YB11, a gene of NF-Y transcription factor family in Triticum aestivum, confers drought tolerance on plants via modulating osmolyte accumulation and reactive oxygen species homeostasis[J]. >Journal of Integrative Agriculture, 2022, 21(11): 3114-3130.
[3] Seong-Woo Cho, Chon-Sik Kang, Hyeon Seok Ko, Byung-Kee Baik, Kwang-Min Cho, Chul Soo Park. Influence of protein characteristics and the proportion of gluten on end-use quality in Korean wheat cultivars[J]. >Journal of Integrative Agriculture, 2018, 17(08): 1706-1719.
No Suggested Reading articles found!