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Journal of Integrative Agriculture  2022, Vol. 21 Issue (1): 91-112    DOI: 10.1016/S2095-3119(20)63490-6
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
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Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine
WANG Pei-pei1, 2*, WANG Zhao-ke1*, GUAN Le2, Muhammad Salman HAIDER2, Maazullah NASIM2, YUAN Yong-bing3, LIU Geng-sen3, LENG Xiang-peng1, 3 
1 Institute of Grape Science and Engineering, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China
2 College of Horticulture, Nanjing Agricultural University, Nanjing 210095, P.R.China
3 Qingdao Key Laboratory of Modern Agriculture Quality and Safety Engineering, College of Horticulture, Qingdao Agricultural University, Qingdao 266109, P.R.China
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Abstract  Nudix hydrolases are widely distributed across all classes of organisms and provide the potential capacity to hydrolyze a wide range of organic pyrophosphates.  Although Nudix hydrolases are involved in plant detoxification processes in response to abiotic and biotic stresses, the biological functions of Nudix hydrolases remain largely unclear in grapevine.  In the present study, a total of 25 putative grapevine Nudix hydrolases (VvNUDXs) were identified by bioinformatics analysis and classified into eight subfamilies based to their preferred substrates.  Both tandem and segmental duplications were responsible for the evolution and expansion of the NUDX gene family in grapevine.  To investigate the regulatory roles of VvNUDX genes during growth and development, as well as in response to abiotic and biotic stresses in grapevine, the expression patterns were revealed in publicly available microarray data.  The spatial and temporal expression patterns of the VvNUDX genes indicated that they might play important roles in multiple developmental processes.  Transcriptome and qRT-PCR analyses showed that ten VvNUDX genes were specifically expressed in grapevine berries, suggesting potential roles in grapevine berry development.  Expression and phylogenetic analyses demonstrated that VvNUDX1 and VvNUDX3 might be involved in terpenoid biosynthesis in grapevine.  Furthermore, most VvNUDX genes active toward the ADP-ribose/NADH showed different patterns in response to various abiotic and biotic stresses, such as salinity and drought, as well as different types of biotic treatments, such as Erysiphe necator, Bois Noir phytoplasma and leaf-roll-associated virus-3 (GLRaV-3).  These results indicated that VvNUDX genes were associated with plant detoxification processes in response to abiotic and biotic stresses, and regulate the disease immunity and resistance pathways.  The information obtained here may provide good opportunities to explore the physiological functions of VvNUDX genes in berry development and stress response networks in grapevine. 
Keywords:  grapevine       NUDX        gene expression        berry development        stress response  
Received: 02 July 2020   Accepted: 16 November 2020
Fund: This work was supported by the National Key Research and Development Program of China (2019YFD1001405-02), the National Natural Science Foundation of China (NSFC; 32002005 and 31801809), the China Postdoctoral Science Foundation (2019M661866), the Breeding Plan of Shandong Provincial Qingchuang Research Team (2019), and the High-level Scientific Research Foundation of Qingdao Agricultural University, China (665/1118011 and 665/1119002).
About author:  0 Correspondence LENG Xiang-peng, E-mail: lengpeng2008@ * These authors contributed equally to this study.

Cite this article: 

WANG Pei-pei, WANG Zhao-ke, GUAN Le, Muhammad Salman HAIDER, Maazullah NASIM, YUAN Yong-bing, LIU Geng-sen, LENG Xiang-peng. 2022. Versatile physiological functions of the Nudix hydrolase family in berry development and stress response in grapevine. Journal of Integrative Agriculture, 21(1): 91-112.

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