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| OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice |
CAO Peng-hui1*, WANG Di2*, GAO Su3*, LIU Xi3, 4#, QIAO Zhong-ying1, XIE Yu-lin1, DONG Ming-hui1, DU Tan-xiao3, ZHANG Xian3, ZHANG Rui3, JI Jian-hui3, 4#
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1 Institute of Agricultural Sciences in Taihu Area of Jiangsu, Suzhou 215155, P.R.China
2 Huaiyin Institute of Agricultural Sciences of Xuhuai Region in Jiangsu, Huai’an 223001, P.R.China
3 School of Life Sciences, Huaiyin Normal University, Huai’an 223300, P.R.China
4 Key Laboratory of Eco-Agricultural Biotechnology around Hongze Lake, Regional Cooperative Innovation Center for Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai’an 223300, P.R.China
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摘要
植物叶绿素生物合成和叶绿体发育是两个受外源和内源因子调控的极其复杂过程。在本研究中，我们鉴定了一个正调控水稻叶绿素生物合成和叶绿体发育的还原异构酶基因OsDXR。OsDXR基因敲除突变体表现为白化致死表型，不能完成整个生命周期。OsDXR在水稻叶片中高表达，亚细胞定位表明OsDXR是一种叶绿体蛋白。与野生型相比，在OsDXR敲除突变体中许多参与叶绿素生物合成和叶绿体发育的基因表达存在差异。我们发现在OsDXR敲除突变体中叶绿体基因ndhA-1019和rpl2-1的RNA编辑效率显著降低。此外，酵母双杂交和双分子荧光互补实验证实，OsDXR与RNA编辑因子OsMORF1有相互作用。我们证实质体2-C-甲基-去甲三醇-4-磷酸途径的破坏导致叶绿体发育和叶绿体基因的RNA编辑存在缺陷。
Abstract
Plant chlorophyll biosynthesis and chloroplast development are two complex processes that are regulated by exogenous and endogenous factors. In this study, we identified OsDXR, a gene encoding a reductoisomerase that positively regulates chlorophyll biosynthesis and chloroplast development in rice. OsDXR knock-out lines displayed the albino phenotype and could not complete the whole life cycle process. OsDXR was highly expressed in rice leaves, and subcellular localization indicated that OsDXR is a chloroplast protein. Many genes involved in chlorophyll biosynthesis and chloroplast development were differentially expressed in the OsDXR knock-out lines compared to the wild type. Moreover, we found that the RNA editing efficiencies of ndhA-1019 and rpl2-1 were significantly reduced in the OsDXR knock-out lines. Furthermore, OsDXR interacted with the RNA editing factor OsMORF1 in a yeast two-hybrid screen and bimolecular fluorescence complementation assay. Finally, disruption of the plastidial 2-C-methyl-derythritol-4-phosphate pathway resulted in defects in chloroplast development and the RNA editing of chloroplast genes.
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Received: 23 November 2022
Accepted: 16 February 2022
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| Fund:
This study was supported by the Program for Subsidized Project of Suzhou Academy of Agricultural Sciences, China (20028), the Science and Technology Foundation of Suzhou (SNG2020048), the Huaishang Talents, China, the National Natural Science Foundation of China (32070345), the Huai’an Academy of Agricultural Sciences Initiation and Development of Scientific Research Fund for High-level Introduced Talents, China (0062019016B), the Six Talents Summit Project of Jiangsu Province, China (NY-129), and the Natural Science Foundation of Jiangsu Province, China (BK20190239 and BK20180107).
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| About author: #Correspondence LIU Xi, E-mail: 1240623244@qq.com; JI Jian-hui, E-mail: jijianhui@hytc.edu.cn
* These authors contributed equally to this work. |
Cite this article:
CAO Peng-hui, WANG Di, GAO Su, LIU Xi, QIAO Zhong-ying, XIE Yu-lin, DONG Ming-hui, DU Tan-xiao, ZHANG Xian, ZHANG Rui, JI Jian-hui.
2023.
OsDXR interacts with OsMORF1 to regulate chloroplast development and the RNA editing of chloroplast genes in rice. Journal of Integrative Agriculture, 22(3): 669-678.
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