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Special Issue:
麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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| Allele mining of wheat ABA receptor at TaPYL4 suggests neo-functionalization among the wheat homoeologs |
| WU Bang-bang1*, SHI Meng-meng1*, Mohammad POURKHEIRANDISH2, ZHAO Qi1, WANG Ying1, YANG Chen-kang1, QIAO Ling1, ZHAO Jia-jia1, YAN Su-xian1, ZHENG Xing-wei1, ZHENG Jun1 |
1 Institute of Wheat Research, Shanxi Agricultural University, Linfen 041000, P.R.China
2 Plant Molecular Biology and Biotechnology Laboratory, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria 3010, Australia
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摘要
本文研究了TaPYL4在中国小麦种质中的单元型分布及其遗传效应。通过克隆小麦TaPYL4的A、B、D基因组序列并分析差异信息，表明TaPYL4-2A在启动子区含有3种单元型，TaPYL4-2B和TaPYL4-2D含有2种单元型。利用262份中国小麦微核心种质和239份山西省小麦品种(系)进行候选基因关联分析，结果表明：TaPYL4-2A与株高和抗旱系数（DTC）显著相关，PYL4-2A-Hap2是降低株高、增加DTC的优异单元型；TaPYL4-2B与穗粒数显著相关，PYL4-2B-Hap1是增加穗粒数的优异单元型，在育种过程中受到人工选择。
Abstract ABA receptors (PYR/PYL/RCAR) play a central role in the water loss control of plants. A previous report indicated that TaPYL4 is a critical gene in wheat that improves grain production under drought conditions and increases water use efficiency. In this study, we analyzed the sequence polymorphisms and genetic effects of TaPYL4s. Based on isolated TaPYL4 genes from chromosomes 2A, 2B and 2D, three haplotypes were detected in the promoter region of TaPYL4-2A, and two haplotypes were present in TaPYL4-2B and TaPYL4-2D, respectively. Marker/trait association analysis indicated that TaPYL4-2A was significantly associated with plant height in 262 Chinese wheat core collection accessions, as well as the drought tolerance coefficient (DTC) for plant height in 239 wheat varieties from Shanxi Province in multiple environments. However, the frequencies of favored drought-tolerant haplotype TaPYL4-2A-Hap2 were considerably low, accounting for only 10%, and lines with this certain Hap could be reserved in the breeding program. TaPYL4-2B was significantly associated with grain number, and the favored haplotype TaPYL4-2B-Hap1 was the dominant allele of above 90% in the collection. For TaPYL4-2D, there were no significant differences in these traits between the two haplotypes in either of the two panels. These results indicate that variation might lead to functional differentiation among the homoeologs and the haplotypes had undergone artificial selection during breeding. Two molecular markers developed to distinguish these haplotypes could be used for breeding in water-limited regions.
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Received: 26 November 2020
Accepted: 02 April 2021
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| Fund: This work was supported by the Agricultural Science Research of Shanxi Academy of Agricultural Sciences, China (YZGC013 and YCX2020BH2), and the Key Research and Development Program of Shanxi Province, China (201803D421021 and 201903D221074). |
| About author: WU Bang-bang, E-mail: wubangbang@sxau.edu.cn; Correspondence ZHENG Jun, Moblie: +86-18835712419, E-mail: sxnkyzj@126.com; ZHENG Xing-wei, Mobile: +86-13467190463, E-mail: smilezxw@126.com
* These authors contributed equally to this study. |
Cite this article:
WU Bang-bang, SHI Meng-meng, Mohammad POURKHEIRANDISH, ZHAO Qi, WANG Ying, YANG Chen-kang, QIAO Ling, ZHAO Jia-jia, YAN Su-xian, ZHENG Xing-wei, ZHENG Jun.
2022.
Allele mining of wheat ABA receptor at TaPYL4 suggests neo-functionalization among the wheat homoeologs. Journal of Integrative Agriculture, 21(8): 2183-2196.
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