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Journal of Integrative Agriculture  2016, Vol. 15 Issue (9): 2153-2162    DOI: 10.1016/S2095-3119(15)61248-5
Soil & Fertilization﹒Irrigation﹒Plant Nutrition﹒ Agro-Ecology & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
The diversity and potential function of endophytic bacteria isolated from Kobreasia capillifolia at alpine grasslands on the Tibetan Plateau, China
WANG Ying, YANG Cheng-de, YAO Yu-ling, WANG Yu-qin, ZHANG Zhen-fen, XUE Li
College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, P.R.China
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Abstract  A total of 50 endophytic bacterial isolates were obtained from Kobreasia capillifolia at alpine grasslands in the Eastern Qilian Mountains on the Tibetan Plateau in China.  Based on the sequencing and phylogenetic analysis of 16S rDNA genes, all isolates phylogenetically related closely to Bacillus, Acinetobacter, Stenotrophomonas, Brevundimonas, Arthrobacter, Curtobacterium, Paenibacillus, Plantibacter, Promicromonospora, Serratia, and Microbacterium, among which Bacillus was the predominant genus (47.8% of the total number of endophytic isolates).  These isolates possessed different biological functions.  In 50 endophytic bacteria, 42 isolates produced indole acetic acid (IAA) on King medium.  There were seven isolates showing potency of mineral phosphate solubilization in Pikovaskaia’s (PKO) liquid medium.  Seven isolates exhibited antagonistic effect against Fusarium avenaceum, Colletotrichum coccodes and Phoma foveata.  This was the first report on diversity and plant growth promotion of endophytic bacteria from K. capillifolia on alpine grassland in the Eastern Qilian Mountains, Chain.  It is essential for revealing the relationship among plant, microorganism, and the special environment because the potential function of endophytic bacteria made a contribution to the interactions of plants and endophytic bacteria.
Keywords:  Tibetan Plateau       Kobreasia capillifolia        endophytic bacteria        diversity        biological functions  
Received: 14 May 2015   Accepted:

We are thankful to the National Natural Science Foundation of China (31160122) and the Key Laboratory of Grassland Ecosystem (Gansu Agricultural University), Ministry of Education (CYzs-2011011) for financial support.

Corresponding Authors:  YANG Cheng-de, Tel/Fax: +86-931-7632161, E-mail:   

Cite this article: 

WANG Ying, YANG Cheng-de, YAO Yu-ling, WANG Yu-qin, ZHANG Zhen-fen, XUE Li. 2016. The diversity and potential function of endophytic bacteria isolated from Kobreasia capillifolia at alpine grasslands on the Tibetan Plateau, China. Journal of Integrative Agriculture, 15(9): 2153-2162.

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