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Journal of Integrative Agriculture  2020, Vol. 19 Issue (4): 1044-1054    DOI: 10.1016/S2095-3119(20)63156-2
Special Issue: Horticulture — Physiology · Biochemistry · Cultivation
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Response of axillary bud development in garlic (Allium sativum L.) to seed cloves soaked in gibberellic acid (GA3) solution
LIU Hong-jiu1, HUANG Cai-ping2, TONG Pei-jiang1, YANG Xue1, CUI Ming-ming1, CHENG Zhi-hui1
1 College of Horticulture, Northwest A&F University, Yangling 712100, P.R.China
2 College of Plant Protection, Northwest A&F University, Yangling 712100, P.R.China
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Abstract  
Gibberellins (GAs) are important phytohormones that regulate many developmental processes in plants.  Clove, as the reproductive organ of garlic, dramatically affected garlic bulb development.  Considering the potential of gibberellins in plant development and our previous studies, we investigated the effect of soaking two types of seed cloves (seed clove-I: without root/shoot sprouting; seed clove-II: with root/shoot sprouting) in GA3 solution on axillary bud development and examined the effect of soaking seed cloves in GA3 solution on bulb development, phytohormone level and sugar content in this study.Results indicated seed clove types, soaking liquids and their interaction significantly affected the number of cloves per bulb and the rate of single-clove bulb.  Moreover, soaking seed cloves in 1 mmol L–1 GA3 solution for 24 h not only promoted axillary bud formation and secondary plant growth (equal to tillering or branching), but also slightly increased the number of cloves per bulb and changed bulb structure with a low yield and marketable quality.  On the 40th day after GA3 treatment (at axillary bud outgrowth stage), zeatin riboside (ZR) and soluble protein in stem were sharply increased with the increase of GA3, sucrose, fructose and soluble protein in leaf.  However, GA3, indole-3-acetic acid (IAA), soluble sugar and sucrose in stem (3.52 ng g–1 fresh weight (FW), 19.88 ng g–1 FW, 237.3 mg g–1 FW, and 8.24 mg g–1 FW, respectively) were significantly decreased on the 40th day after GA3 treatment, compared to the control of water treatment (5.56 ng g–1 FW, 32.96 ng g–1 FW, 263.6 mg g–1 FW, and 10.37 mg g–1 FW, respectively).  To our knowledge, these novel results indicate seed cloves soaked in GA3 solution promotes axillary bud formation and outgrowth that caused the changes in plant architecture and bulb structure.  Meanwhile, our findings suggest that the level of endogenous plant hormone (GA3, IAA and ZR) cooperates with the content of sugar (sucrose and fructose) in leaf and stem to regulate axillary bud outgrowth in garlic.
Keywords:  garlic (Allium sativum)        gibberellic acid        seed clove        axillary bud        plant hormone        sugar  
Received: 11 September 2019   Accepted: 04 March 2020
Fund: This research was supported by the grants from the National Natural Science Foundation of China (31772293) and the Education Development Fund Project of Northwest A&F University, China (2017).
Corresponding Authors:  Correspondence CHENG Zhi-hui, E-mail: chengzh@nwsuaf.edu.cn   
About author:  LIU Hong-jiu, E-mail: liured9@nwsuaf.edu.cn;

Cite this article: 

LIU Hong-jiu, HUANG Cai-ping, TONG Pei-jiang, YANG Xue, CUI Ming-ming, CHENG Zhi-hui. 2020. Response of axillary bud development in garlic (Allium sativum L.) to seed cloves soaked in gibberellic acid (GA3) solution. Journal of Integrative Agriculture, 19(4): 1044-1054.

Alexopoulos A A, Akoumianakis K A, Olympios C M, Passam H C. 2007. The effect of the time and mode of application of gibberellic acid and inhibitors of gibberellin biosynthesis on the dormancy of potato tubers grown from true potato seed. Journal of the Science of Food and Agriculture, 87, 1973–1979.
Alexopoulos A A, Akoumianakis K A, Passam H C. 2006a. Effect of plant growth regulators on the tuberisation and physiological age of potato (Solanum tuberosum L.) tubers grown from true potato seed. Canadian Journal of Plant Science, 86, 1217–1225.
Alexopoulos A A, Akoumianakis K A, Passam H C. 2006b. The effect of the time and mode of application of gibberellic acid on the growth and yield of potato plants derived from true potato seed. Journal of the Science of Food and Agriculture, 86, 2189–2195.
Alexopoulos A A, Karapanos I C, Akoumianakis K A, Passam H C. 2017. Effect of gibberellic acid on the growth rate and physiological age of tubers cultivated from true potato seed. Journal of Plant Growth Regulation, 36, 1–10.
Barbier F, Perez-Garcia M D, Barrière Q, Sakr S, Lecerf M, Péron T, Bertheloot J, Rol?ík J, Boutet-Mercey S, Citerne S, Porcheron B, Lemoine R, Roman H, Le Gourrierec J, Leduc N. 2015. Sucrose is an early modulator of the key hormonal mechanisms controlling bud outgrowth in Rosa hybrida. Journal of Experimental Botany, 66, 2569–2582.
Barbier F F, Dun E A, Kerr S C, Chabikwa T G, Beveridge C A. 2019. An update on the signals controlling shoot branching. Trends in Plant Science, 24, 220–236.
Barbier F F, Lunn J E, Beveridge C A. 2015. Ready, steady, go! A sugar hit starts the race to shoot branching. Current Opinion in Plant Biology, 25, 39–45.
Bennett T, Leyser O. 2006. Something on the side: Aaxillary meristems and plant development. Plant Molecular Biology, 60, 843–854.
Bonhomme M, Peuch M, Ameglio T, Rageau R, Guilliot A, Decourteix M, Alves G, Sakr S, Lacointe A. 2009. Carbohydrate uptake from xylem vessels and its distribution among stem tissues and buds in walnut (Juglans regia L.). Tree Physiology, 30, 89–102.
Bradford M M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72, 248–254.
Bredmose N, Kristiansen K, Nørbæk R, Christensen L P, Hansen-Møller J. 2005. Changes in concentrations of cytokinins (CKs) in root and axillary bud tissue of miniature rose suggest that local CK biosynthesis and zeatin-type CKs play important roles in axillary bud growth. Journal of Plant Growth Regulation, 24, 238–250.
Cavagnaro P F, Galmarini C R. 2007. Garlic. In: Kole C, ed., Vegetables. Springer Berlin Heidelberg, Berlin, Heidelberg.pp. 349–364.
Chen X J, Xia X J, Guo X, Zhou Y H, Shi K, Zhou J, Yu J Q. 2016. Apoplastic H2O2 plays a critical role in axillary bud outgrowth by altering auxin and cytokinin homeostasis in tomato plants. New Phytologist, 211, 1266–1278.
Claeys H, De Bodt S, Inzé D. 2014. Gibberellins and DELLAs: Central nodes in growth regulatory networks. Trends in Plant Science, 19, 231–239.
Domingos S, Nobrega H, Raposo A, Cardoso V, Soares I, Ramalho J C, Leitão A E, Oliveira C M, Goulao L F. 2016. Light management and gibberellic acid spraying as thinning methods in seedless table grapes (Vitis vinifera L.): Cultivar responses and effects on the fruit quality. Scientia Horticulturae, 201, 68–77.
DuBois M, Gilles K A, Hamilton J K, Rebers P A, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350–356.
Elfving D C, Visser D B, Henry J L. 2011. Gibberellins stimulate lateral branch development in young sweet cherry trees in the orchard. International Journal of Fruit Science, 11, 41–54.
Erogul D, Sen F. 2015. Effects of gibberellic acid treatments on fruit thinning and fruit quality in Japanese plum (Prunus salicina Lindl.). Scientia Horticulturae, 186, 137–142.
Fambrini M, Mariotti L, Parlanti S, Picciarelli P, Salvini M, Ceccarelli N, Pugliesi C. 2011. The extreme dwarf phenotype of the GA-sensitive mutant of sunflower, dwarf2, is generated by a deletion in the ent-kaurenoic acid oxidase1 (HaKAO1) gene sequence. Plant Molecular Biology, 75, 431–450.
Fambrini M, Mariotti L, Parlanti S, Salvini M, Pugliesi C. 2015. A GRAS-like gene of sunflower (Helianthus annuus L.) alters the gibberellin content and axillary meristem outgrowth in transgenic Arabidopsis plants. Plant Biology, 17, 1123–1134.
Henry C, Rabot A, Laloi M, Mortreau E, Sigogne M, Leduc N, Lemoine R, Sakr S, Vian A, Pelleschi-travier S. 2011. Regulation of RhSUC2, a sucrose transporter, is correlated with the light control of bud burst in Rosa sp. Plant, Cell and Environment, 34, 1776–1789.
Hosseini A, Hosseinzadeh H. 2015. A review on the effects of Allium sativum (Garlic) in metabolic syndrome. Journal of Endocrinological Investigation, 38, 1147–1157.
Hu W, Sun D W, Blasco J. 2017. Rapid monitoring 1-MCP-induced modulation of sugars accumulation in ripening ‘Hayward’ kiwifruit by Vis/NIR hyperspectral imaging. Postharvest Biology and Technology, 125, 168–180.
Iwahori S, Weaver R J, Pool R M. 1968. Gibberellin-like activity in berries of seeded and seedless Tokay grapes. Plant Physiology, 43, 333–337.
Janssen B J, Drummond R S M, Snowden K C. 2014. Regulation of axillary shoot development. Current Opinion in Plant Biology, 17, 28–35.
Jiang B, Miao H, Chen S, Zhang S, Chen F, Fang W. 2010. The lateral suppressor-like gene, DgLsL, alternated the axillary branching in transgenic chrysanthemum (Chrysanthemum×morifolium) by modulating IAA and GA content. Plant Molecular Biology Reporter, 28, 144–151.
Kamenetsky R. 2007. Garlic: Botany and horticulture. Horticultural Reviews, 33, 123–172.
Leyser O. 2009. The control of shoot branching: An example of plant information processing. Plant, Cell & Environment, 32, 694–703.
Li J, Yu X, Lou Y, Wang L, Slovin J P, Xu W, Wang S, Zhang C. 2015. Proteomic analysis of the effects of gibberellin on increased fruit sink strength in Asian pear (Pyrus pyrifolia). Scientia Horticulturae, 195, 25–36.
Liu H, Deng R, Huang C, Cheng Z, Meng H. 2019a. Exogenous gibberellins alter morphology and nutritional traits of garlic (Allium sativum L.) bulb. Scientia Horticulturae, 246, 298–306.
Liu H, Zhang Y, Yang F, Qi X, Ahmad H, Wu C, Cheng Z. 2019b. Effect of the mode and time of gibberellic acid treatment on plant architecture and bulb structure in garlic (Allium sativum L.). Scientia Horticulturae, 257, 108723.
Lv X, Zhang M, Wu Y, Gao X, Li X, Wang W. 2017. The roles of auxin in regulating “shoot branching” of Cremastra appendiculata. Journal of Plant Growth Regulation, 36, 281–289.
Maboko M M, Du Plooy C P. 2015. Effect of plant growth regulators on growth, yield, and quality of sweet pepper plants grown hydroponically. Hortscience, 50, 383–386.
Marquat C, Vandamme M, Gendraud M, Pétel G. 1999. Dormancy in vegetative buds of peach: Relation between carbohydrate absorption potentials and carbohydrate concentration in the bud during dormancy and its release. Scientia Horticulturae, 79, 151–162.
Mauriat M, Sandberg L G, Moritz T. 2011. Proper gibberellin localization in vascular tissue is required to control auxin-dependent leaf development and bud outgrowth in hybrid aspen. The Plant Journal, 67, 805–816.
Ben Michael T, Shemesh-Mayer E, Kimhi S, Gershberg C, Forer I, de Avila V T, Rabinowitch H D, Goldstein R K. 2018. Temporal and spatial effect of low pre-planting temperatures on plant architecture and flowering in bolting garlic. Scientia Horticulturae, 242, 69–75.
Ni J, Gao C C, Chen M S, Pan B Z, Ye K Q, Xu Z F. 2015. Gibberellin promotes shoot branching in the perennial woody plant Jatropha curcas. Plant and Cell Physiology, 56, 1655–1666.
Rabot A, Henry C, Ben Baaziz K, Mortreau E, Azri W, Lothier J, Hamama L, Boummaza R, Leduc N, Pelleschi-Travier S, Le Gourrierec J, Sakr S. 2012. Insight into the role of sugars in bud burst under light in the rose. Plant and Cell Physiology, 53, 1068–1082.
Rabot A, Portemer V, Péron T, Mortreau E, Leduc N, Hamama L, Coutos-Thévenot P, Atanassova R, Sakr S, Le Gourrierec J. 2014. Interplay of sugar, light and gibberellins in expression of Rosa hybrida vacuolar invertase 1 regulation. Plant and Cell Physiology, 55, 1734–1748.
Rahman M H, Haque M S, Karim M, Ahmed M. 2006. Effects of gibberellic acid (GA3) on breaking dormancy in garlic (Allium sativum L.). International Journal of Agriculture and Biology, 8, 63–65.
Resende J T V D, Morales R G F, Resende F V, Faria M V, Souza R J D, Marchese A. 2011. Garlic vernalization and planting dates in Guarapuava. Horticultura Brasileira, 29, 193–198.
Rizza A, Jones A M. 2019. The makings of a gradient: spatiotemporal distribution of gibberellins in plant development. Current Opinion in Plant Biology, 47, 9–15.
Rohkin Shalom S, Gillett D, Zemach H, Kimhi S, Forer I, Zutahy Y, Tam Y, Teper-Bamnolker P, Kamenetsky R, Eshel D. 2015. Storage temperature controls the timing of garlic bulb formation via shoot apical meristem termination. Planta, 242, 951–962.
Wakchaure G C, Minhas P S, Meena K K, Singh N P, Hegade P M, Sorty A M. 2018. Growth, bulb yield, water productivity and quality of onion (Allium cepa L.) as affected by deficit irrigation regimes and exogenous application of plant bio-regulators. Agricultural Water Management, 199, 1–10.
Wang G L, Que F, Xu Z S, Wang F, Xiong A S. 2015. Exogenous gibberellin altered morphology, anatomic and transcriptional regulatory networks of hormones in carrot root and shoot. BMC Plant Biology, 15, 290.
Wang Y, Wang J, Shi B, Yu T, Qi J, Meyerowitz E M, Jiao Y. 2014. The stem cell niche in leaf axils is established by auxin and cytokinin in Arabidopsis. The Plant Cell, 26, 2055–2067.
Wu C N, Wang M Y, Cheng Z H, Meng H W. 2016. Response of garlic (Allium sativum L.) bolting and bulbing to temperature and photoperiod treatments. Biology Open, 5, 507–518.
Wu C N, Wang M Y, Dong Y X, Cheng Z H, Meng H W. 2015. Growth, bolting and yield of garlic (Allium sativum L.) in response to clove chilling treatment. Scientia Horticulturae, 194, 43–52.
Yamazaki H, Shiraiwa N, Itai A, Honda I. 2015. Involvement of gibberellins in the regulation of tillering in Welsh onion (Allium fistulosum L.). Horticulture Journal, 84, 334–341.
Yang C, Liu J, Dong X, Cai Z, Tian W, Wang X. 2014. Short-term and continuing stresses differentially interplay with multiple hormones to regulate plant survival and growth. Molecular Plant, 7, 841–855.
Yang J, Zhang J, Wang Z, Zhu Q, Wang W. 2001. Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiology, 127, 315–323.
Yang Y M, Xu C N, Wang B M, Jia J Z. 2001. Effects of plant growth regulators on secondary wall thickening of cotton fibres. Plant Growth Regulation, 35, 233–237.
Zhao J, Li G, Yi G X, Wang B M, Deng A X, Nan T G, Li Z H, Li Q X. 2006. Comparison between conventional indirect competitive enzyme-linked immunosorbent assay (icELISA) and simplified icELISA for small molecules. Analytica Chimica Acta, 571, 79–85.
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