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Journal of Integrative Agriculture  2019, Vol. 18 Issue (10): 2183-2192    DOI: 10.1016/S2095-3119(18)62118-5
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Application of virus-induced gene silencing for identification of FHB resistant genes
FAN Yan-hui1*, HOU Bing-qian1*, SU Pei-sen1, WU Hong-yan2, WANG Gui-ping1, KONG Ling-rang1, MA Xin1, WANG Hong-wei1
1 State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2 Shandong Agricultural University Fertilizer Science Technology Co., Ltd., Feicheng 271600, P.R.China
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Virus-induced gene silencing (VIGS) showed several advantages to identify gene functions such as short experimental cycle, more broad hosts, etc.  In this study, the feasibility and efficiency of employing Barley stripe mosaic virus (BSMV)-based VIGS system to evaluate Fusarium head blight (FHB) resistance were explored in wheat.  With variable conditions tested, it showed that the maximal silencing efficiency 78% on spike was obtained when the recombinant BSMV was inoculated on flag leaf at flagging stage.  However, the plant may reduce its own immunity to FHB when inoculated with BSMV.  To induce this impact, different Fusarium graminearum strains were tested and SF06-1 strain was selected for FHB resistance evaluation.  Using this system, TaAOC, TaAOS, and TaOPR3 involved in jasmonic acid (JA) signaling pathway were identified to positively regulate FHB resistance, which was underpinned by the results when silencing TaAOS in wheat by stable transgenic plants.
Keywords:  Triticeae        Fusarium head blight (FHB)        BSMV-VIGS        RNAi  
Received: 04 June 2018   Accepted: 29 September 2019
Fund: This work was supported by the National Natural Science Foundation of China (3315203911 and 31471488), the National Key Research and Development Program of China (2016YFD0100602), the Transgenic Special Item, China (2016ZX08002003-002 and 2016ZX08009-003), and the Bohai Granary Science and Technology Demonstration Project of Shandong Province, China (2017BHLC020).
Corresponding Authors:  Correspondence WANG Hong-wei, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail:; MA Xin, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail:    
About author:  * These authors contributed equally to this study.

Cite this article: 

FAN Yan-hui, HOU Bing-qian, SU Pei-sen, WU Hong-yan, WANG Gui-ping, KONG Ling-rang, MA Xin, WANG Hong-wei. 2019. Application of virus-induced gene silencing for identification of FHB resistant genes. Journal of Integrative Agriculture, 18(10): 2183-2192.

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