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Journal of Integrative Agriculture  2019, Vol. 18 Issue (10): 2193-2204    DOI: 10.1016/S2095-3119(18)62140-9
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Dynamic changes of root proteome reveal diverse responsive proteins in maize subjected to cadmium stress
REN Wen1, 2, LIU Ya2, ZHOU Miao-yi2, SHI Zi2, WANG Tian-yu1, ZHAO Jiu-ran2, LI Yu
1 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Maize Research Center, Beijing Academy of Agriculture & Forestry Sciences/Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, P.R.China
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Abstract  
Toxic symptoms and tolerance mechanisms of heavy metal in maize are well documented.  However, limited information is available regarding the changes in the proteome of maize seedling roots in response to cadmium (Cd) stress.  Here, we employed an iTRAQ-based quantitative proteomic approach to characterize the dynamic alterations in the root proteome during early developmental in maize seedling.  We conducted our proteomic experiments in three-day seedling subjected to Cd stress, using roots in four time points.  We identified a total of 733, 307, 499, and 576 differentially abundant proteins after 12, 24, 48, or 72 h of treatment, respectively.  These proteins displayed different functions, such as ribosomal synthesis, reactive oxygen species homeostasis, cell wall organization, cellular metabolism, and carbohydrate and energy metabolism.  Of the 166 and 177 proteins with higher and lower abundance identified in at least two time points, 14 were common for three time points.  We selected nine proteins to verify their expression using quantitative real-time PCR.  Proteins involved in the ribosome pathway were especially responsive to Cd stress.  Functional characterization of the proteins and the pathways identified in this study could help our understanding of the complicated molecular mechanism involved in Cd stress responses and create a list of candidate gene responsible for Cd tolerance in maize seeding roots.
Keywords:  cadmium stress        iTRAQ proteomics        maize seedling roots  
Received: 08 June 2018   Accepted:
Fund: This work was supported by the Foundation for Young Scientist of Beijing Academy of Agriculture & Forestry Sciences, China (QNJJ201505) and the National Key Research and Development Program of China (SQ2016ZY03002163).
Corresponding Authors:  Correspondence LI Yu, Tel: +86-10-62131196, E-mail: liyu03@caas.cn; ZHAO Jiu-ran, Tel: +86-10-51503936, Fax: +86-10-51503404, E-mail: maizezhao@126.com    

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REN Wen, LIU Ya, ZHOU Miao-yi, SHI Zi, WANG Tian-yu, ZHAO Jiu-ran, LI Yu. 2019. Dynamic changes of root proteome reveal diverse responsive proteins in maize subjected to cadmium stress. Journal of Integrative Agriculture, 18(10): 2193-2204.

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