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Journal of Integrative Agriculture  2019, Vol. 18 Issue (4): 900-906    DOI: 10.1016/S2095-3119(19)62591-8
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Relationship between copulation and cold hardiness in Ophraella communa (Coleoptera: Chrysomelidae)
ZHAO Chen-chen*, YUE Lei*, WANG Yao, GUO Jian-ying, ZHOU Zhong-shi, WAN Fang-hao
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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Ophraella communa (Coleoptera: Chrysomelidae), the ragweed leaf beetle, is a biological control agent of the invasive common ragweed, Ambrosia artemisiifolia (Asterales: Asteraceae).  Adults can survive cold conditions that occur during winter.  The adults mate before entering overwintering.  Understanding the connection between copulation and overwintering will be useful for determining O. communa seasonality.  Determining the relationship between overwintering and copulation required comparison of mated and unmated beetles at mean lethal temperature (LTemp50) exposures for 2 h.  Cold-related physiological indices, including the water ratio, super cooling point (SCP), cryoprotectant levels, and energy reserve levels, were also measured.  Mating treatment decreased the LTemp50 of both sexes by reducing their mean SCP and water ratios.  Although the changes of cryoprotectant levels in mated adults were not precisely consistent in between the genders, they increased greatly in both males and females.  Body sugar may play a role in copulation and may also elevate cold hardiness in O. communa
Keywords:  Ophraella communa        mating status        cold tolerance        hibernation       super cooling point        energy reserves  
Received: 25 June 2018   Accepted:
Fund:  This study was supported by the National Natural Science Foundation of China (31322046). 
Corresponding Authors:  Correspondence GUO Jian-ying, E-mail:; ZHOU Zhong-shi, Tel: +86-10-62810159, E-mail:    
About author:  ZHAO Chen-chen, E-mail:; YUE Lei, E-mail:;

Cite this article: 

ZHAO Chen-chen, YUE Lei, WANG Yao, GUO Jian-ying, ZHOU Zhong-shi, WAN Fang-hao. 2019. Relationship between copulation and cold hardiness in Ophraella communa (Coleoptera: Chrysomelidae). Journal of Integrative Agriculture, 18(4): 900-906.

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