Please wait a minute...
Journal of Integrative Agriculture  2017, Vol. 16 Issue (11): 2646-2654    DOI: 10.1016/S2095-3119(16)61622-2
Food Science Advanced Online Publication | Current Issue | Archive | Adv Search |
The effect of dehydrogenase enzyme activity in glycolysis on the colour stability of mutton during postmortem storage
XIN Jian-zeng1, 2, LI Zheng2, LI Xin2, LI Meng2, WANG Ying2, YANG Fu-min1, ZHANG De-quan2
1 College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, P.R.China
2 Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  This study investigated the influence of activities of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and lactate dehydrogenase-B (LDH-B) on the colour stability of mutton.  From 60 sheep (Bayannur mutton sheep), 15 longissimus dorsi (LD) muscles were selected on the basis of colour stability (R630/580 and a* value) during the storage and classified into three groups (5 for each group) as high colour stability (HCS), intermediate colour stability (ICS) and low colour stability (LCS).  The activities of GAPDH and LDH-B, muscle colour attributes, nicotinamide adenine dinucleuotide (NADH) concentration and lactate concentration were measured.  The samples in HCS had higher activities of GAPDH and LDH-B than the samples in the LCS, and the samples in the HCS group also possessed higher NADH and lower lactate concentration.  The higher activity of dehydrogenase enzyme may result in higher NADH concentrations and colour stability in muscle tissue.  The results suggest that the activity of GAPDH and LDH-B may also play a role in maintaining colour stability.
Keywords:  mutton        meat colour        GAPDH              LDH-B        NADH  
Received: 27 December 2016   Accepted:
Fund: 

We gratefully acknowledge financial support from the National Agricultural Science and Technology Innovation Program, China, the Special Fund for Agro-scientific Research in the Public Interest in China (201303083) and the earmarked fund for China Agriculture Research System (CARS-39).

Corresponding Authors:  Correspondence YANG Fu-min, E-mail: yfumin@163.com; ZHANG De-quan, E-mail: dequan_zhang0118@126.com   
About author:  XIN Jian-zeng, E-mail: jianzeng77@sina.com

Cite this article: 

XIN Jian-zeng, LI Zheng, LI Xin, LI Meng, WANG Ying, YANG Fu-min, ZHANG De-quan. 2017. The effect of dehydrogenase enzyme activity in glycolysis on the colour stability of mutton during postmortem storage. Journal of Integrative Agriculture, 16(11): 2646-2654.

Baibai T, Oukhattar L, Moutaouakkil A, Soukri A. 2007. Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from european pilchard sardina pilchardus. Acta Biochimica et Biophysica Sinica, 39, 947–954.

Bao Y, Puolanne E, Ertbjerg P. 2016. Effect of oxygen concentration in modified atmosphere packaging on color and texture of beef patties cooked to different temperatures. Meat Science, 121, 189–195.

Barron J T, Gu L, Parrillo J E. 1998. Malate-aspartate shuttle, cytoplasmic nadh redox potential, and energetics in vascular smooth muscle. Journal of Molecular & Cellular Cardiology, 30, 1571–1579.

Calnan H, Jacob R H, Pethick D W, Gardner G E. 2016. Production factors influence fresh lamb longissimus colour more than muscle traits such as myoglobin concentration and pH. Meat Science, 119, 41–50.

Canto A C, Costa-Lima B R, Suman S P, Monteiro M L, Viana F M, Salim A P, Nair M N, Silva T J, Conte-Junior C A. 2016. Color attributes and oxidative stability of longissimus lumborum and psoas major muscles from Nellore bulls. Meat Science, 121, 19–26.

Canto A C, Suman S P, Nair M N, Li S, Rentfrow G, Beach C M, Silva T J, Wheeler T L, Shackelford S D, Grayson A, McKeith R O, King D A. 2015. Differential abundance of sarcoplasmic proteome explains animal effect on beef Longissimus lumborum color stability. Meat Science, 102, 90–98.

Gao X G, Xie L, Wang Z Y, Li X M, Luo H L, Ma C W, Dai R T. 2013. Effect of postmortem time on the metmyoglobin reductase activity, oxygen consumption, and colour stability of different lamb muscles. European Food Research and Technology, 236, 579–587.

Hunt M C, King A. 2012. AMSA Meat Color Measurement Guidelines. American Meat Science Association. Champaign, Illinois, USA 61820. pp. 1–135.

Joseph P, Suman S P, Rentfrow G, Li S, Beach C M. 2012. Proteomics of muscle-specific beef color stability. Journal of Agricultural & Food Chemistry, 60, 3196–3203.

Kim Y H. 2008. Lactate dehydrogenase regulation of the metmyoglobin reducing system to improve color stability of bovine muscles through lactate enhancement. Dissertation Abstracts International, 69, 1–115.

Kim Y H, Hunt M C, Mancini R A, Seyfert M, Loughin T M, Kropf D H, Smith J S. 2006. Mechanism for lactate-color stabilization in injection-enhanced beef. Journal of Agricultural & Food Chemistry, 54, 7856–7862.

Kim Y H, Keeton J T, Smith S B, Berghman L R, Savell J W. 2009a. Role of lactate dehydrogenase in metmyoglobin reduction and color stability of different bovine muscles. Meat Science, 83, 376–382.

Kim Y H, Keeton J T, Yang H S, Smith S B, Sawyer J E, Savell J W. 2009b. Color stability and biochemical characteristics of bovine muscles when enhanced with L- or D-potassium lactate in high-oxygen modified atmospheres. Meat Science, 82, 234–240.

King D A, Shackelford S D, Kuehn L A, Kemp C M, Rodriguez A B, Thallman R M, Wheeler T L. 2010. Contribution of genetic influences to animal-to-animal variation in myoglobin content and beef lean color stability. Journal of Animal Science, 88, 1160–1167.

King D A, Shackelford S D, Rodriguez A B, Wheeler T L. 2011. Effect of time of measurement on the relationship between metmyoglobin reducing activity and oxygen consumption to instrumental measures of beef longissimus color stability. Meat Science, 87, 26–32.

Klingenberg M. 1974. Nicotinamide-adenine dinucleotides (NAD, NADP, NADH, NADPH) spectrophotometric and fluorimetric methods. In: Methods of Enzymatic Analysis. 2nd ed. Academic Press, New York and London. pp. 2045–2072.

Ledward D A. 1985. Post-slaughter influences on the formation of metyyoglobin in beef muscles. Meat Science, 15, 149–171.

Li W, Sauve A A. 2015. NAD(+) content and its role in mitochondria. Methods in Molecular Biology, 1241, 39–48.

Luciano G, Monahan F J, Vasta V, Pennisi P, Bella M, Priolo A. 2009. Lipid and colour stability of meat from lambs fed fresh herbage or concentrate. Meat Science, 82, 193–199.

Matarneh S K, England E M, Scheffler T L, Oliver E M, Gerrard D E. 2015. Net lactate accumulation and low buffering capacity explain low ultimate pH in the longissimus lumborum of AMPKγ3R200Q mutant pigs. Meat Science, 110, 189–195.

McCormick D B, Wright L D. 1971. Nicotinic acid: Analogs and coenzymes. In: Colowick S P, Kaplan N O, eds., Methods in Enzymology. vol. 18. Academic Press, New York and London. pp. 26–27.

McKenna D R, Mies P D, Baird B E, Pfeiffer K D, Ellebracht J W, Savell J W. 2005. Biochemical and physical factors affecting discoloration characteristics of 19 bovine muscles. Meat Science, 70, 665–682.

Neethling N E, Suman S P, Sigge G O, Hoffman L C. 2016. Muscle-specific colour stability of blesbok (Damaliscus pygargus phillipsi) meat. Meat Science, 119, 69–79.

Ramanathan R, Mancini R A, Dady G A. 2011. Effects of pyruvate, succinate, and lactate enhancement on beef longissimus raw color. Meat Science, 88, 424–428.

Ramanathan R, Mancini R A, Joseph P, Yin S, Tatiyaborworntham N, Petersson K H, Sun Q, Konda M R. 2011. Effects of lactate on ground lamb color stability and mitochondria-mediated metmyoglobin reduction. Food Chemistry, 126, 166–171.

Richards M P. 2013. Redox reactions of myoglobin. Antioxid Redox Signal, 18, 2342–2351.

Saleh B, Watts B M. 1968. Substrates and intermediates in the enzymatic reduction of metmyoglobin in ground beef. Journal of Food Science, 33, 353–358.

Suman S P, Joseph P. 2013. Myoglobin chemistry and meat color. Annual Review of Food Science and Technology, 4, 79–99.

Yin M C, Faustman C. 1993. Influence of temperature, pH, and phospholipid composition upon the stability of myoglobin and phospholipid a liposome model. Journal of Agricultural & Food Chemistry, 41, 853–857.
[1] Xiaoxu Shen, Yongtong Tian, Wentao He, Can He, Shunshun Han, Yao Han, Lu Xia, Bo Tan, Menggen Ma, Houyang Kang, Jie Yu, Qing Zhu, Huadong Yin. Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-β signaling[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2764-2777.
[2] Wajjiha Batool, Justice Norvienyeku, Wei Yi, Zonghua Wang, Shihong Zhang, Lili Lin. Disruption of non-classically secreted protein (MoMtp) compromised conidiation, stress homeostasis, and pathogenesis of Magnaporthe oryzae[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2686-2702.
[3] Gaozhao Wu, Xingyu Chen, Yuguang Zang, Ying Ye, Xiaoqing Qian, Weiyang Zhang, Hao Zhang, Lijun Liu, Zujian Zhang, Zhiqin Wang, Junfei Gu, Jianchang Yang. An optimized strategy of nitrogen-split application based on the leaf positional differences in chlorophyll meter readings[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2605-2617.
[4] Xiaogang He, Zirong Li, Sicheng Guo, Xingfei Zheng, Chunhai Liu, Zijie Liu, Yongxin Li, Zheming Yuan, Lanzhi Li. Epistasis-aware genome-wide association studies provide insights into the efficient breeding of high-yield and high-quality rice[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2541-2556.
[5] Jie Deng, Zi’e Wang, Wenyun Li, Xiaohua Chen, Diqiu Liu. WRKY11 up-regulated dirigent expression to enhance lignin/lignans accumulation in Lilium regale Wilson during response to Fusarium wilt[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2703-2722.
[6] Li Cui, Jianguo Wang, Zhaohui Tang, Zheng Zhang, Sha Yang, Feng Guo, Xinguo Li, Jingjing Meng, Jialei Zhang, Yakov Kuzyakov, Shubo Wan. General and specialized metabolites in peanut roots regulate arbuscular mycorrhizal symbiosis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2618-2632.
[7] Yao Zhang, Zelong She, Ruolan He, Shuangyan Yao, Xiang Li, Xiaoguang Liu, Xinming Yin, Jizhen Wei, Mengfang Du, Shiheng An. The Ca2+/CaN/ACC and cAMP/PKA/HK signal pathways are required for PBAN-mediated sex pheromone biosynthesis in Conogethes punctiferalis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2735-2751.
[8] Zihui Liu, Xiangjun Lai, Yijin Chen, Peng Zhao, Xiaoming Wang, Wanquan Ji, Shengbao Xu. Selection and application of four QTLs for grain protein content in modern wheat cultivars[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2557-2570.
[9] Gensheng Zhang, Mudi Sun, Xinyao Ma, Wei Liu, Zhimin Du, Zhensheng Kang, Jie Zhao. Yr5-virulent races of Puccinia striiformis f. sp. tritici possess relative parasitic fitness higher than current main predominant races and potential risk[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2674-2685.
[10] Ming Ju, Guiting Li, Qiuzhen Tian, Hengchun Cao, Qin Ma, Yinghui Duan, Hui Guo, Zhanyou Zhang, Yingying Huang, Huili Wang, Haiyang Zhang, Hongmei Miao. Deletion of a 1,049 bp sequence from the 5´ UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame  [J]. >Journal of Integrative Agriculture, 2024, 23(8): 2589-2604.
[11] Xiaotong Liu, Siwei Liang, Yijia Tian, Xiao Wang, Wenju Liang, Xiaoke Zhang. Effect of land use on soil nematode community composition and co-occurrence network relationship[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2807-2819.
[12] Jia Chen, Xinran Zhang, Ziqi He, Dongwei Xiong, Miao Long. Damage on intestinal barrier function and microbial detoxification of deoxynivalenol: A review[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2507-2524.
[13] Yuhan Zhao, Chen Qian, Yumei Zhang, Xiande Li, Kamiljon T. Akramov. Food security amid the COVID-19 pandemic in Central Asia: Evidence from rural Tajikistan[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2853-2867.
[14] Myeong-Hyeon Min, Aye Aye Khaing, Sang-Ho Chu, Bhagwat Nawade, Yong-Jin Park. Exploring the genetic basis of pre-harvest sprouting in rice through a genome-wide association study-based haplotype analysis[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2525-2540.
[15] Xin Zhang, Jingjing Wang, Fuli Tan, Haixiu Gao, Shenggen Fan. The potential impact of increased whole grain consumption among Chinese adults on reducing healthcare costs and carbon footprint[J]. >Journal of Integrative Agriculture, 2024, 23(8): 2842-2852.
No Suggested Reading articles found!