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Journal of Integrative Agriculture
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Multi-omic analysis for dietary supplementation of different ratios of soluble and insoluble fiber on intestinal microbiota, metabolites and inflammation of weaned piglets
Yetong Xu1*, Chengyu Zhou1*, Yingying Lu1, Xutong Guo1, Minyue Zong1, Junwei Zhu1, Pan Zhou2, Jiaman Pang1, Xie Peng1,  Zhihong Sun1# #br#
1 Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and Technology, Southwest University, Chongqing 400715, China
2 School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China

 Highlights 

Different SDF:IDF ratios showed limited effects on growth and intestinal microbiota of weaned piglets.

Dietary fiber supplementation with different SDF:IDF ratios affected intestinal integrity in weaned piglets.

A SDF:IDF ratio of 0.25 upregulated beneficial metabolites and downregulated certain peptides, improving intestinal integrity.

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摘要  

背景和目的:不同类型的日粮纤维在改善动物肠道健康和整体表现方面发挥着重要作用,但对补充不同比例的可溶性和不可溶性纤维SDF:IDF)与肠道炎症的关系、其对微生物群和代谢物的影响以及潜在机制的全面评估仍不清楚。本研究旨在研究不同SDF:IDF比例高纤维日粮对断奶仔猪的生长性能、肠道屏障完整性、微生物群及代谢物组成的影响。方法:选用28日龄断奶仔猪,饲喂日粮包括基础日粮CON)和三种高纤维日粮SDF:IDF比例分别为0.37、0.25和0.13(HF-0.37、HF-0.25和HF-0.13组),每个处理组10个重复,每个重复2头猪,试验期28天。结果:纤维处理对断奶仔猪生长性能没有显著影响,第 14 天,HF-0.37 组仔猪的干物质表观消化率显著高于 HF-0.25 组和 HF-0.13 组(P < 0.05)。在第14天和第28天,HF-0.37组的结肠肿瘤坏死因子-α、白介素(IL)-1β、IL-6和IL-8的含量较CON、HF-0.25和HF-0.13组显著增加(P < 0.05)。第14天和第28天,HF-0.25组的血浆D-乳酸和内毒素水平较CON组显著降低(P < 0.05),而HF-0.25和HF-0.13组的仔猪结肠紧密连接蛋白zonula occludens 1表达与CON和HF-0.37组相比在第14天时上调(P < 0.05)。在转录水平上,三种高纤维日粮影响了肠道中与炎症和免疫反应相关的信号通路。HF-0.25组在第14天上调了仔猪结肠中的3-吲哚丁酸、烟酸和3-甲基硫丙胺水平,并在第28天降低了一些小肽的水平。结论: HF-0.25日粮纤维组,对仔猪的肠道完整性具有积极影响,这可能与结肠中肠道代谢物的变化有关,而HF-0.37和HF-0.13日粮纤维组对断奶仔猪的肠道完整性影响有限。创新性本研究通过综合微生物组学、转录组学和代谢组学分析,创新性地探索了不同比例的可溶性和不可溶性膳食纤维对断奶仔猪肠道完整性的影响。



Abstract  
Different types of dietary fiber (DF) play important roles in improving the intestinal health and overall performance of animals. The study aimed to investigate the effects of high DF with different ratios of soluble and insoluble DF (SDF:IDF) on the growth performance, intestinal barrier integrity, microbiota and metabolite profiles of weaned piglets. The four dietary treatments included basal diet (CON) and three high DF diets with 0.37, 0.25 and 0.13 SDF:IDF ratios (HF-0.37, HF-0.25 and HF-0.15 groups). On day 14 and 28, colonic tumor necrosis factor -α, interleukin (IL)-1β, IL-6 and IL-8 contents were increased with the HF-0.37 group than the CON, HF-0.25 and HF-0.13 groups (P < 0.05). The plasma D-lactate and endotoxin levels were reduced with the HF-0.25 group compared to CON group on day 14 and 28 (P < 0.05), while colonic zonula occludens 1 expression of piglets was upregulated with HF-0.25 and HF-0.13 compared to the CON and HF-0.37 groups on day 14 (P < 0.05). At transcription level, three high DF diets affected signaling pathways related to inflammation and immune responses in the colon of piglets. DF supplementation especially HF-0.25 upregulated colonic 3-indole butyric acid, nicotinic acid and 3-methylthiopropylamine levels on day 14 and reduced some peptides levels on day 28. These findings revealed that DF supplementation especially HF-0.25 showed beneficial effects on intestinal integrity of piglets which could be related to the changes of intestinal metabolites in colon, while HF-0.37 and HF-0.13 had limited effects on intestinal integrity of weaned piglets.



Keywords:  Weaned piglets       Soluble fiber       Insoluble fiber       Multi-omics       Intestinal integrity         
Online: 15 May 2025  
Fund: 

This work was financially supported by Chongqing Municipal Education Commission Projects (4322400156) and Sichuan Science and Technology Program (2022YFH0064).

About author:  #Correspondence: email address: sunzh2002cn@aliyun.com (Z Sun). *Contributed equally with first author.

Cite this article: 

Yetong Xu, Chengyu Zhou, Yingying Lu, Xutong Guo, Minyue Zong, Junwei Zhu, Pan Zhou, Jiaman Pang, Xie Peng, Zhihong Sun. 2025. Multi-omic analysis for dietary supplementation of different ratios of soluble and insoluble fiber on intestinal microbiota, metabolites and inflammation of weaned piglets. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.05.013

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