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Journal of Integrative Agriculture  2016, Vol. 15 Issue (12): 2703-2711    DOI: 10.1016/S2095-3119(16)61465-X
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage
LUO Xiang-dong1, ZHAO Jun1, DAI Liang-fang1, ZHANG Fan-tao1, ZHOU Yi1, WAN Yong2, XIE Jian-kun1
1 College of Life Science, Jiangxi Normal University, Nanchang 330022, P.R.China
2 Rice Research Institute, Jiangxi Academy of Agricultural Science, Nanchang 330200, P.R.China
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Abstract      Cold stress is one of the major restraints for rice production. Cold tolerance is controlled by complex genetic factor. In this study, a backcross inbred lines (BILs) population derived from an inter-specific cross (Oryza sativa L.×O. rufipogon Griff.) was used for genetic linkage map construction and quantitative trait locus (QTL) mapping. A linkage map consisting of 153 markers was constructed, spanning 1 596.8 cM with an average distance of 11.32 cM between the adjacent markers. Phenotypic evaluation of the parents and BILs under (6±1)°C cold stress revealed that the ability of cold tolerance in BILs at early seedling obeyed a skewed normal and continuous distribution. Fifteen QTLs on chromosomes 6, 7, 8, 11, and 12 were identified using survival percent (SP) and non death percent (NDP) as indicators of cold tolerance, which could explain 5.99 to 40.07% of the phenotypic variance, of which the LOD values ranged from 3.04 to 11.32. Four QTLs on chromosomes 3, 5 and 7 were detected using leaf conductivity (LC) and root conductivity (RC) as indicators of cold tolerance, ranging from 19.54 to 33.53% for the phenotypic variance explained and 2.54 to 6.12 for the LOD values. These results suggested that there might be multi major QTLs in O. rufipogon and some useful genes for cold tolerance have been transferred into cultivated rice, which would be helpful for cloning and utilizing the cold tolerance-responsive genes from wild rice.
Keywords:  common wild rice        cold tolerance        quantitative trait loci (QTL)        introgression  
Received: 08 April 2016   Accepted:

This research was partially supported by the National Natural Science Foundation of China (31260255, 31360327 and 31360147), the Development Program for Young Scientists of Jiangxi Province, China (20112BCB23007) and the Scientific Planning Project of Jiangxi Provincial Education Department, China (GJJ12184 and KJLD12059).

Corresponding Authors:  LUO Xiang-dong, Tel: +86-791-88120390, E-mail:; XIE Jian-kun, Tel: +86-791-88120396, E-mail:   

Cite this article: 

LUO Xiang-dong, ZHAO Jun, DAI Liang-fang, ZHANG Fan-tao, ZHOU Yi, WAN Yong, XIE Jian-kun. 2016. Linkage map construction and QTL mapping for cold tolerance in Oryza rufipogon Griff. at early seedling stage. Journal of Integrative Agriculture, 15(12): 2703-2711.

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