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Journal of Integrative Agriculture  2016, Vol. 15 Issue (9): 1933-1943    DOI: 10.1016/S2095-3119(15)61214-X
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice (Oryza rufipogon Griff.)
ZHAO Jie, QIN Jing-jing, SONG Qian, SUN Chuan-qing, LIU Feng-xia
State Key Laboratory of Plant Physiology and Biochemistry, National Center for Evaluation of Agricultural Wild Plants (Rice)/ Beijing Key Laboratory of Crop Genetic Improvement/Department of Plant Genetics and Breeding, China Agricultural University, Beijing 100193, P.R.China
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Abstract  Rice (Oryza sativa L.), a tropical and subtropical crop, is susceptible to low temperature stress during seedling, booting, and flowering stages, which leads to lower grain quality levels and decreasing rice yields. Cold tolerance is affected by multiple genetic factors in rice, and the complex genetic mechanisms associated with chilling stress tolerance remain unclear. Here, we detected seven quantitative trait loci (QTLs) for cold tolerance at booting stage and identified one cold tolerant line, SIL157, in an introgression line population derived from a cross between the indica variety Guichao 2, as the recipient, and Dongxiang common wild rice, as the donor. When compared with Guichao 2, SIL157 showed a stronger cold tolerance during different growth stages. Through an integrated strategy that combined QTL-mapping with expression profile analysis, six candidate genes, which were up-regulated under chilling stress at the seedling and booting developmental stages, were studied. The results may help in understanding cold tolerance mechanisms and in using beneficial alleles from wild rice to improve the cold tolerance of rice cultivars through molecular marker-assisted selection.
Keywords:  common wild rice        introgression line       low temperature tolerance        different growth stages        candidate genes  
Received: 26 August 2015   Accepted:

This research was supported by the National Natural Science Foundation of China (31371585 and 30971755) and the Beijing Youth Talent, China (31056102).

Corresponding Authors:  LIU Feng-xia, Tel/Fax: +86-10-62733450, E-mail:   

Cite this article: 

ZHAO Jie, QIN Jing-jing, SONG Qian, SUN Chuan-qing, LIU Feng-xia. 2016. Combining QTL mapping and expression profile analysis to identify candidate genes of cold tolerance from Dongxiang common wild rice (Oryza rufipogon Griff.). Journal of Integrative Agriculture, 15(9): 1933-1943.

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