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| Proteome analysis of alfalfa roots in response to water deficit stress |
| Rahman Md Atikur1, Kim Yong-Goo1, Alam Iftekhar1, 3, LIU Gong-she2, Lee Hyoshin4, Lee Jeung Joo5, Lee Byung-Hyun1 |
1 Division of Applied Life Sciences (BK21Plus), Institute of Agriculture & Life Sciences (IALS), Gyeongsang National University, Jinju 660-701, Republic of Korea
2 Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P.R.China
3 National Institute of Biotechnology, Dhaka-1349, Bangladesh
4 Division of Forest Biotechnology, Korea Forest Research Institute, Suwon 441-847, Republic of Korea
5 Department of Applied Biology, Institute of Agriculture & Life Sciences (IALS), Gyeongsang National University, Jinju 660-701, Republic of Korea |
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Abstract To evaluate the response of alfalfa to water deficit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, followed by 3 days re-watering. Water deficit increased H2O2 content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identified in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identified several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identified proteins are involved in a variety of cellular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxification/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.
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Received: 14 May 2015
Accepted:
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| Fund: This work was supported by the National Research Foundation of Korea (NRF) Grant (NRF-2011-616-F00013). Alam I was supported by post-doctoral grant, and Rahman M A, Kim Y-G were supported by the scholarship from BK21Plus program, Ministry of Education, Republic of Korea. |
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Corresponding Authors:
Lee Byung-Hyun, Tel: +82-55-7721882,
Fax: +82-55-7721889, E-mail: hyun@gnu.ac.kr
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| About author: Rahman Md Atikur, E-mail: atikgnu@gmail.com |
Cite this article:
Rahman Md Atikur, Kim Yong-Goo, Alam Iftekhar, LIU Gong-she, Lee Hyoshin, Lee Jeung Joo, Lee Byung-Hyun.
2016.
Proteome analysis of alfalfa roots in response to water deficit stress. Journal of Integrative Agriculture, 15(06): 1275-1285.
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