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Journal of Integrative Agriculture  2016, Vol. 15 Issue (11): 2571-2577    DOI: 10.1016/S2095-3119(16)61336-9
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site
QIAN Li-li1, MA De-zun2, GAO Peng-fei2, JIANG Sheng-wang2, WANG Qing-qing2, CAI Chun-bo1, XIAO Gao-jun2, AN Xiao-rong1*, CUI Wen-tao2*
1 State Key Laboratory of Agrobiotechnology, China Agricultural University, Beijing 100193, P.R.China
2 State Key Laboratory for Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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Abstract      Myostatin, a member of the transforming growth factor beta (TGF-β) superfamily, is a dominant inhibitor that acts to limit skeletal muscle growth and development. In this study, we generated transgenic mice that express porcine myostatin containg mutations at its cleavage site (RSRR) to evaluate its effect on muscle mass. Results showed that the weight of four skeletal muscles including gastrocnemius, rectus femoris, tibialis anterior, and pectoralis increased by 17.83 and 28.39%, 21.76 and 28.70%, 34.31 and 41.62%, 53.21 and 27.54% in transgenic male and female mice, respectively, compared to their corresponding non-transgenic control mice. Measurement of muscle fiber size and number indicated that the mean myofiber size increased by 50.73 and 61.30% in transgenic male and female mice respectively compared to the non-transgenic controls. However, there was no difference in the number of myofiber between transgenic and non-transgenic male mice. These results clearly demonstrated that the increase in skeletal muscle mass in transgenic mice is caused by hypertrophy instead of hyperplasia.
Keywords:  porcine myostatin        muscle mass        transgenic mice        mutation        hypertrophy        hyperplasia  
Received: 31 December 2015   Accepted:

This work was supported by the National Natural Science Foundation of China (30901022), the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS05), and the National Basic Research Program of China (2015CB943100).

Corresponding Authors:  CUI Wen-tao, Tel/Fax: +86-10-62819480, E-mail:; AN Xiao-rong, Tel/Fax: +86-10-62733355, E-mail:   
About author:  QIAN Li-li, E-mail:

Cite this article: 

QIAN Li-li, MA De-zun, GAO Peng-fei, JIANG Sheng-wang, WANG Qing-qing, CAI Chun-bo, XIAO Gao-jun, AN Xiao-rong, CUI Wen-tao. 2016. Muscle hypertrophy in transgenic mice due to over-expression of porcine myostatin mutated at its cleavage site. Journal of Integrative Agriculture, 15(11): 2571-2577.

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