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The complete genome sequence of Citrus vein enation virus from China |
HUANG Ai-jun, SONG Zhen, CAO Meng-ji, CHEN Hong-ming, LI Zhong-an, ZHOU Chang-yong |
1、Citrus Research Institute, Chinese Academy of Agricultural Sciences/Southwest University, Chongqing 400712, P.R.China
2、College of Plant Protection, Southwest University, Chongqing 400716, P.R.China |
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摘要 The complete nucleotide sequence of an isolate of Citrus vein enation virus (CVEV-XZG) from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames (ORFs), had a similar genomic organization features with Pea enation mosaic virus (PEMV). Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.
Abstract The complete nucleotide sequence of an isolate of Citrus vein enation virus (CVEV-XZG) from China has been determined for the first time. The genome consisted of 5 983 nucleotides, coding for five open reading frames (ORFs), had a similar genomic organization features with Pea enation mosaic virus (PEMV). Nucleotide and deduced amino acid sequence identity of the five ORFs compared to isolate CVEV VE-1 range from 97.1 to 99.0% and 97.4 to 100.0%, these values compared to isolate PEMV-1 range from 45.2 to 51.6% and 31.1 to 45.2%. Phylogenetic analysis based on the complete genome sequence showed that the isolate CVEV-XZG had close relationship with Pea enation mosaic virus. The results supports CVEV may be a new member of genus Enamovirus. The full sequence of CVEV-XZG presented here may serve as a basis for future study of CVEV in China.
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Received: 15 July 2014
Accepted:
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Fund: This research is funded by the Chongqing Natural Science Foundation Project, China (cstc2011jjA80025). |
Corresponding Authors:
ZHOU Chang-yong, Tel: +86-23-68349007, E-mail: zhoucy@cric.cn
E-mail: zhoucy@cric.cn
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About author: HUANG Ai-jun, Mobile: +86-15123804062, E-mail: hijhk123@163.com; |
Cite this article:
HUANG Ai-jun, SONG Zhen, CAO Meng-ji, CHEN Hong-ming, LI Zhong-an, ZHOU Chang-yong.
2015.
The complete genome sequence of Citrus vein enation virus from China. Journal of Integrative Agriculture, 14(3): 598-601.
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Chen G Q, Wang H X, Yan S X, Roistacher C N. 1996.Occurrence and detection of Citrus vein enation virus inHuangyan, Zhejiang, China. In: Proceedings of the 13thConference of the International Organization of CitrusVirologists. Fuzhou, China.Chen G Q, Yan S X , Roistacher C N. 1992. First report of citrusvein enation disease in China. Plant Disease, 76, 1077.Fraser L R. 1960. Woody gall, a suspected virus disease ofrough lemon and other citrus varieties. Proceedings of theLinnean Society of New South Wales, 84, 332-334Fusaro A F, Correa R L, Nakasugi K, Jackson C, Kawchuk L,Vaslin M F S, Waterhouse P M. 2012. The EnamovirusP0 protein is a silencing suppressor which inhibits localand systemic RNA silencing through AGO1 degradation.Virology, 426, 178-187Giedroc D P, Cornish P V. 2009. Frameshifting RNApseudoknots: Structure and mechanism. Virus Research,139, 193-208da Graça J V, Maharaj S B. 1991. Citrus vein enation virus, aprobable luteovirus. In: Proceedings of the 11th Conferenceof the International Organization of Citrus Virologists.University of California, Riverside, USA.Jacomino A P, Salibe A A. 1993. Occurrence of woody galldisease in citrus in siio paulo state, Brazil. In: Proceedingsof the 12th Conference of the International Organization ofCitrus Virologists. University of California, Riverside, USA.Maharaj S B, da Graça J V. 1988. Observation of isometric viruslikeparticles associated with citrus vein enation-infectedcitrus and the viruliferous aphid vector Toxoptera citricidus.Phytophylactica, 20, 357-360Schmitz J, Stussi-Garaud C, Tacke E, Prüfer D, Rohde W,Rohfritsch O. 1997. In situ localization of the putativemovement protein (pr17) from Potato leafroll luteovirus(PLRV) in infected and transgenic potato plants. Virology,235, 311-322de Segura C B, Ferrand B A. 1969. Woody galls, its distributionand importance in new and old citrus plantings in Peru.Proceeding of First International Citrus Symposium, 3,1449-1451Vives M C, Velázquez K, Pina J A, Moreno P, Guerri J, NavarroL. 2013. Identification of a new enamovirus associated withcitrus vein enation disease by deep sequencing of smallRNAs. Phytopathology, 103, 1077-1086Wallace J M, Deake R. 1953. A virus-induced vein enation incitrus. Citrus Leaves, 33, 22-24Wallace J M, Deake R. 1960. Woody galls on citrus associatedwith vein enation virus infection. Plant Disease Reporter,44, 580-584 |
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