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| First detection and complete genome of Soybean chlorotic mottle virus naturally infecting soybean in China by deep sequencing |
HU Qian-qian1, LIU Xue-jian1, HAN Xue-dong1, LIU Yong2, JIANG Jun-xi3, XIE Yan1 |
1 State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, P.R.China
2 Hunan Plant Protection Institute, Changsha 410125, P.R.China
3 College of Agriculture, Jiangxi Agricultural University, Nanchang 330000, P.R.China |
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Abstract Soybean chlorotic mottle virus (SbCMV) was first detected from soybean plants in Jiangxi Province of China by high throughput sequencing and was confirmed by PCR. The complete nucleotide sequence of NC113 was determined to be 8 210 nucleotides, and shared the highest similarity (91.7%) with sequences of SbCMV that was only reported in Japan. It encodes nine putative open reading frames (ORFs Ia, Ib and II–VIII), and contains a large intergenic region located at nucleotide 5 976–6 512 between ORFs VI and VII. Sequence analysis and phylogenetic tree indicated that NC113 is an isolate of SbCMV, and is more related to the soymoviruses Blueberry red ringspot virus (BRRSV), Peanut chlorotic streak virus (PCSV) and Cestrum yellow leaf curling virus (CmYLCV) than to other representative members in the Caulimoviridae family. Field survey of 472 legume plants from Jiangxi and Zhejiang provinces showed SbCMV was only detected from soybean in Nanchang City with a low incidence rate. This is the first report of Soybean chlorotic mottle virus identified in China.
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Received: 06 November 2018
Accepted:
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| Fund: This work was supported by the Special Fund for Agro-Scientific Research in the Public Interest, China (201303028) and the National Natural Science Foundation of China (31571977). |
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Corresponding Authors:
Correspondence XIE Yan, Tel: +86-571-88982677, E-mail: xiey@zju.edu.cn
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| About author: HU Qian-qian, E-mail: yimonu@163.com; |
Cite this article:
HU Qian-qian, LIU Xue-jian, HAN Xue-dong, LIU Yong, JIANG Jun-xi, XIE Yan .
2019.
First detection and complete genome of Soybean chlorotic mottle virus naturally infecting soybean in China by deep sequencing. Journal of Integrative Agriculture, 18(11): 2664-2667.
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