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Journal of Integrative Agriculture  2016, Vol. 15 Issue (7): 1667-1671    DOI: 10.1016/S2095-3119(16)61343-6
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Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China
GAO Rui, LI Shi-fang, LU Mei-guang
State Key Laboratory of Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China
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Abstract      Cherry virus A (CVA) is a member of the genus Capillovirus, in the family Betaflexiviridae. The infection rate of CVA was high in sweet cherry in China. We determined the complete nucleotide sequences of two isolates of CVA from Tai’an, Shandong Province, China using high fidelity PCR enzymes and specific primer pairs for amplifying long fragments in RT-PCR and RACE. The full-length sequences from isolates ChTA11 and ChTA12 are both 7 382 nucleotide (nt) long, excluding the poly(A) tail, encode two open reading frames (ORFs) and have similar genome organization to the two isolates in GenBank. The complete nucleotide sequence of ChTA11 is 98.2 and 81.2% nt identity to the isolates from Germany and India in GenBank, respectively, and the ChTA12 isolate is 98.2 and 81.0% similar. Analysis of the nucleotide and amino acid sequences showed that the domain of unknown function (DUF1717) is more variable compared with other domains. This is the first report of the complete nucleotide sequences of CVA isolates infecting sweet cherry in China.
Keywords:  Cherry virus A (CVA)        genome sequence        Capillovirus  
Received: 02 September 2015   Accepted:
Fund: 

This work was supported by the Special Fund for Agro-scientific Research in the Public Interest, China (201203076) and the National Natural Science Foundation of China (31471752).

Corresponding Authors:  LU Mei-guang, Tel: +86-10-62815615, E-mail: mglu@ippcaas.cn   
About author:  GAO Rui, E-mail: jiaoxiaoleng@163.com

Cite this article: 

GAO Rui, LI Shi-fang, LU Mei-guang. 2016. Complete nucleotide sequences of two isolates of Cherry virus A from sweet cherry in China. Journal of Integrative Agriculture, 15(7): 1667-1671.

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