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| Identification and validation of root-specific promoters in rice |
| HUANG Li-yu, ZHANG Fan, QIN Qiao, WANG Wen-sheng, ZHANG Ting, FU Bin-ying |
| National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China |
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摘要 Novel promoters that confer root-specific expression would be useful for engineering resistance against problems of nutrient and water absorption by roots. In this study, the reverse transcriptase polymerase chain reaction was used to identify seven genes with root-specific expression in rice. The isolation and characterization of upstream promoter regions of five selected genes rice root-specific promoter (rRSP) 1 to 5 (rRSP1-rRSP5) and A2P (the promoter of OsAct2) revealed that rRSP1, rRSP3, and rRSP5 are particularly important with respect to root-specific activities. Furthermore, rRSP1, rRSP3, and rRSP5 were observed to make different contributions to root activities in various species. These three promoters could be used for root-specific enhancement of target gene(s).
Abstract Novel promoters that confer root-specific expression would be useful for engineering resistance against problems of nutrient and water absorption by roots. In this study, the reverse transcriptase polymerase chain reaction was used to identify seven genes with root-specific expression in rice. The isolation and characterization of upstream promoter regions of five selected genes rice root-specific promoter (rRSP) 1 to 5 (rRSP1-rRSP5) and A2P (the promoter of OsAct2) revealed that rRSP1, rRSP3, and rRSP5 are particularly important with respect to root-specific activities. Furthermore, rRSP1, rRSP3, and rRSP5 were observed to make different contributions to root activities in various species. These three promoters could be used for root-specific enhancement of target gene(s).
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Received: 03 January 2014
Accepted:
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| Fund: This work was supported by the National Natural Science Foundation of China (31271694) and the National Transgenic Plant Program of China (2011ZX08001-003). |
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Corresponding Authors:
FU Bin-ying, Tel: +86-10-82106698, Fax: +86-10-82108559,E-mail: fubinying@caas.cn
E-mail: fubinying@caas.cn
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| About author: HUANG Li-yu, E-mail: lyhuang2007@163.com; |
Cite this article:
HUANG Li-yu, ZHANG Fan, QIN Qiao, WANG Wen-sheng, ZHANG Ting, FU Bin-ying.
2015.
Identification and validation of root-specific promoters in rice. Journal of Integrative Agriculture, 14(1): 1-10.
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| Bailey T L, Boden M, Buske F A, Frith M, Grant C E, ClementiL, Ren J, Li W W, Noble W S. 2009. MEME SUITE: Toolsfor motif discovery and searching. Nucleic Acids Research,37, W202-W208.Benedict C, Skinner J S, Meng R, Chang Y, Bhalerao R,Huner N P, Finn C E, Chen T H, Hurry V. 2006. The CBF1-dependent low temperature signaling pathway, regulon andincrease in freeze tolerance are conserved in Populus spp.Plant, Cell & Environment, 29, 1259-1272Benfey P N, Chua N H. 1989. Regulated genes in transgenicplants. Science, 244, 174-181Benfey P N, Chua N H. 1990. The cauliflower mosaic virus 35Spromoter: combinatorial regulation of transcription in plants.Science, 250, 959-966Christ A, Maegele I, Ha N, Nguyen H H, Crespi M D, Maizel A.2012. In silico identification and in vivo validation of a setof evolutionary conserved plant root-specific cis-regulatoryelements. Mechanisms of Development, 130, 70-81Christensen A H, Sharrock R A, Quai P H. 1992. Maizepolyubiquitin genes: Structure, thermal perturbation ofexpression and transcript splicing, and promoter activityfollowing transfer to protoplasts by electroporation. PlantMolecular Biology, 18, 675-689Cornejo M J, Luth D, Blankenship K M, Anderson O D. 1993.Activity of a maize ubiquitin promoter in transgenic rice.Plant Molecular Biology, 23, 567-581Curtis M D, Grossniklaus U. 2003. A gateway cloning vector setfor high-throughput functional analysis of genes in planta.Plant Physiology, 133, 462-469Elmayan T, Tepfer M. 1995. Evaluation in tobacco of the organspecificity and strength of the rolD promoter, domain Aof the 35S promoter and the 35S promoter. TransgenicResearch, 4, 388-396Fei H, Chaillou S, Hirel B, Mahon J D, Vessey J K. 2003.Overexpression of a soybean cytosolic glutaminesynthetase gene linked to organ-specific promoters in peaplants grown in different concentrations of nitrate. Planta,216, 467-474Fraisier V, Gojon A, Tillard P, Daniel-Vedele F. 2000.Constitutive expression of a putative high-affinity nitratetransporter in Nicotiana plumbaginifolia: Evidence for posttranscriptionalregulation by a reduced nitrogen source. ThePlant Journal, 23, 489-496Fukai S, Cooper M. 1995. Development of drought-resistantcultivars using physiomorphological traits in rice. FieldCrops Research, 40, 67-86Gilmour S J, Selbot A M, Salazar M P, Everar J D, ThomashowM F. 2000. Overexpression of the Arabidopsis CBF3transcriptional activator mimics multiple biochemicalchanges associated with cold acclimation. Plant Physiology,124, 1854-1865Gowik U, Burscheidt J, Akyildiz M, Schlue U, Koczor M,Streubel M, Westhoff P. 2004. cis-Regulatory elements formesophyll-specific gene expression in the C4 plant Flaveriatrinervia, the promoter of the C4 phosphoenolpyruvatecarboxylase gene. The Plant Cell, 16, 1077-1090He C, Lin Z, McElroy D, Wu R. 2009. Identification of a riceActin2 gene regulatory region for high-level expression oftransgenes in monocots. Plant Biotechnology Journal, 7,227-239Hellens R P, Allan A C, Friel E N, Bolitho K, Grafton K,Templeton M D, Karunairetnam S, Gleave A P, Laing W A.2005. Transient expression vectors for functional genomics,quantification of promoter activity and RNA silencing inplants. Plant Methods, 1, 13.Higo K, Ugawa Y, Iwamoto M, Korenaga T. 1999. Plant cisactingregulatory DNA elements (PLACE) database. NucleicAcids Research, 27, 297-300Hsieh T H, Lee J T, Yang P T, Chiu L H, Charng Y Y, Wang Y C,Chan M T. 2002. Heterology expression of the ArabidopsisC-repeat/dehydration response element binding factor1gene confers elevated tolerance to chilling and oxidativestress in transgenic tomato. Plant Physiology, 129, 1086-1094Jang I C, Choi W B, Lee K H, Song S I, Nahm B H, Kim J K. 2002.High-level and ubiquitous expression of the rice cytochromec gene OsCc1 and its promoter activity in transgenic plantsprovides a useful promoter for transgenesis of monocots.Plant Physiology, 129, 1473-1481Jeon J S, Lee S, Jung K H, Jun S H, Kim C, An G. 2000.Tissue-preferential expression of a rice-tubulin gene,OsTubA1, mediated by the first intron. Plant Physiology,123, 1005-1014Jeong J S, Kim Y S, Baek K H, Jung H, Ha S H, Do C Y, KimM, Reuzeau C, Kim J K. 2010. Root-specific expressionof OsNAC10 improves drought tolerance and grain yieldin rice under field drought conditions. Plant Physiology,153, 185-197Kasuga M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K.2004. A combination of Arabidopsis DREB1A gene andstress inducible rd29A promoter improved drought and lowtemperature stress tolerance in tobacco by gene transfer.Plant & Cell Physiology, 45, 346-350Keller B, Baumgartner C. 1991. Vascular-specific expressionof the bean Grp-18 gene is negatively regulated. The PlantCell, 3, 1639-1646Leach F, Aoyagi K. 1991. Promoter analysis of the highlyexpressed rolC and rolD root-inducing genes ofAgrobacterium rhizogenes: Enhancer and tissue-specificDNA determinants are dissociated. Plant Science, 79,69-76Li Y Y, Liu S J, Yu Z M, Liu Y, Wu P. 2013. Isolation andcharacterization of two novel root-specific promoters in rice(Oryza sativa L.). Plant Science, 204, 37-44Liu H T, Yu X H, Li K W, Klejnot J, Yang H Y, Lisiero D, Lin CT. 2008. Photoexcited CRY2 interacts with CIB1 to regulatetranscription and floral initiation in Arabidopsis. Science,322, 1535-1539Lu J, Sivamani E, Li X, Qu R. 2008. Activity of the 5´ regulatoryregions of the rice polyubiquitin rubi3 gene in transgenic riceplants as analysed by both GUS and GFP reporter genes.Plant Cell Reports, 27, 1587-1600McElroy D, Blowers A D, Jenes B, Wu R. 1991. Construction ofexpression vectors based on the rice Actin1 (Act1) 5´ regionfor use in monocot transformation. Molecular Genetics andGenomics, 231, 150-160Park S H, Yi N, Kim Y S, Jeong M H, Bang S W, Choi Y D, KimJ K. 2010. Analysis of five novel putative constitutive genepromoters in transgenic rice plants. Journal of ExperimentalBotany, 61, 2459-2467Pino M T, Skinner J S, Park E J, Jeknic Z, Hayes P M,Thomashow M F, Chen T H. 2007. Use of a stress induciblepromoter to drive ectopic AtCBF expression improves potatofreezing tolerance while minimizing negative effects on tuberyield. Plant Biotechnology Journal, 5, 591-604Potenza C, Aleman L, Sengupta-Gopalan C. 2004. Targetingtransgene expression in research, agricultural, andenvironmental applications: Promoters used in planttransformation. In Vitro Cellular & Developmental Biology,40, 1-22Sahoo K K, Tripathi A K, Pareek A, Sopory S K, Singla-PareekS L. 2011. An improved protocol for efficient transformationand regeneration of diverse indica rice cultivars. PlantMethods, 7, 49.Wang D, Pan J, Zhao Q, Zhu H, Fu Y, Li K. 2011. Genomewidetemporal-spatial gene expression profiling of droughtresponsiveness in rice. BMC Genomics, 12, 149.Wang J, Oard J H. 2003. Rice ubiquitin promoters: deletionanalysis and potential usefulness in plant transformationsystems. Plant Cell Reports, 22, 129-134Wilmink A, van de Ven B C E, Dons J J. 1995. Activity ofconstitutive promoters in various species from the Liliaceae.Plant Molecular Biology, 28, 949-955Xu Y, Buchholz W G, DeRose R T, Hall T C. 1995.Characterization of a rice gene family encoding root-specificproteins. Plant Molecular Biology, 27, 237-248Yamamoto Y T, Taylor C G, Acedo G N, Cheng C L, Conkling MA. 1991. Characterization of cis-acting sequences regulatingroot-specific gene expression in tobacco. The Plant Cell,3, 371-382Yoshida S, Forno D, Cock J, Ka G. 1976. Laboratory Manualfor Physiological Studies of Rice. The International RiceResearch Institute, Manila, the PhilippinesYu S M, Ko S S, Hong C Y, Sun H J, Hsing Y I, Tong C G, HoT H. 2007. Global functional analyses of rice promotersby genomics approaches. Plant Molecular Biology, 65,417-425Yue B, Xue W, Xiong L, Yu X, Luo L, Cui K, Jin D, Xing Y, ZhangQ. 2006. Genetic basis of drought resistance at reproductivestage in rice: Separation of drought tolerance from droughtavoidance. Genetics, 172, 1213-1228Zhao G, Schriefer L A, Stormo G D. 2007. Identification ofmuscle-specific regulatory modules in Caenorhabditiselegans. Genome Research, 17, 348-357 |
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