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| Expression Comparisons of Pathogenesis-Related (PR) Genes in Wheat in Response to Infection/Infestation by Fusarium, Yellow dwarf virus (YDV) Aphid-Transmitted and Hessian Fly |
| WU Shi-wen, WANG Hong-wei, YANG Zai-dong , KONG Ling-rang |
1、State Key Laboratory of Crop Biology/College of Agronomy, Shandong Agricultural University, Tai’an 271018, P.R.China
2、Department of Agronomy, Purdue University, West Lafayette, IN 47907, USA |
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摘要 Expression profiles of ten pathogenesis-related (PR) genes during plant defense against Fusarium, Yellow dwarf virus (YDV) aphid-transmitted and Hessian fly (Hf) were compared temporally in both resistant and susceptible genotypes following pathogen infection or insect infestation. Quantitative real-time PCR (qRT-PCR) revealed that PR1, PR2, PR3, PR5, PR6, PR8, PR9, and PR15 appeared to be induced or suppressed independently in response to Fusarium, YDV aphid-transmitted or Hf during the interactions. The PR gene(s) essential to defense against one organism may play little or no role in defense against another pathogen or pest, suggesting the alternative mechanisms may be involved in different interactions of wheat- Fusarium, wheat-YDV aphid-transmitted and wheat-Hf. However, strong up- or down-regulation of PR12 and PR14 encoding low molecular membrane acting protein, defensin and lipid transfer protein (LTP), respectively, had been detected after either pathogen infection or insect infestation, therefore showed broad responses to pathogens and insects. It was postulated that low molecular proteins such as defensins and LTPs might play a role in the early stages of pathogenesis in the signaling process that informs plants about the attack from biotic stresses. In addition, a synergistic action between different PR genes might exist in plants to defense certain pathogens and insects on the basis of comprehensive expression profiling of various pathogenesis-related genes revealed by qRT-PCR in this study.
Abstract Expression profiles of ten pathogenesis-related (PR) genes during plant defense against Fusarium, Yellow dwarf virus (YDV) aphid-transmitted and Hessian fly (Hf) were compared temporally in both resistant and susceptible genotypes following pathogen infection or insect infestation. Quantitative real-time PCR (qRT-PCR) revealed that PR1, PR2, PR3, PR5, PR6, PR8, PR9, and PR15 appeared to be induced or suppressed independently in response to Fusarium, YDV aphid-transmitted or Hf during the interactions. The PR gene(s) essential to defense against one organism may play little or no role in defense against another pathogen or pest, suggesting the alternative mechanisms may be involved in different interactions of wheat- Fusarium, wheat-YDV aphid-transmitted and wheat-Hf. However, strong up- or down-regulation of PR12 and PR14 encoding low molecular membrane acting protein, defensin and lipid transfer protein (LTP), respectively, had been detected after either pathogen infection or insect infestation, therefore showed broad responses to pathogens and insects. It was postulated that low molecular proteins such as defensins and LTPs might play a role in the early stages of pathogenesis in the signaling process that informs plants about the attack from biotic stresses. In addition, a synergistic action between different PR genes might exist in plants to defense certain pathogens and insects on the basis of comprehensive expression profiling of various pathogenesis-related genes revealed by qRT-PCR in this study.
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Received: 20 March 2013
Accepted:
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| Fund: the National Basic Research Program of China (2009CB118301), the Transgenic Special Item of China (2011ZX08002-004 and 2011ZX08009-003), the National Natural Science Foundation of China (31071405), and the National High- Tech R&D Program of China (2011AA100102 and 2012AA101105). |
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Corresponding Authors:
KONG Ling-rang, Tel: +86-538-8249278, Fax: +86-538-8242226, E-mail: lkong@sdau.edu.cn
E-mail: lkong@sdau.edu.cn
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| About author: WU Shi-wen, E-mail: wushiwen0036@163.com |
Cite this article:
WU Shi-wen, WANG Hong-wei, YANG Zai-dong , KONG Ling-rang.
2014.
Expression Comparisons of Pathogenesis-Related (PR) Genes in Wheat in Response to Infection/Infestation by Fusarium, Yellow dwarf virus (YDV) Aphid-Transmitted and Hessian Fly. Journal of Integrative Agriculture, 13(5): 926-936.
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