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| Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm |
| HE Yu-xuan, LI Hong-yan, ZHANG Yong, HE Jian-hua, ZHANG Hong, ZHAO Xing-xu |
1.College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, P.R.China
2.Department of Medical Physics, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, P.R.China
3.Key Laboratory of Heavy Ion Radiation Medicine, Chinese Academy of Sciences, Lanzhou 730000, P.R.China
4.Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Lanzhou 730000, P.R.China
5.University of Chinese Academy of Sciences, Beijing 100049, P.R.China
6.Lanzhou Agricultural Science and Technology Research Promotion Center, Lanzhou 730030, P.R.China |
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摘要 This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis (2-DE) analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry (LC-MS/MS). Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases (NCBI) indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha (TFAP-2α). The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.
Abstract This study is first to investigate proteomic changes in sheep sperm induced by carbon ion radiation using two-dimensional electrophoresis (2-DE) analysis in the project of breeding a new variety of sheep. Differential expression proteins were detected using the PDQuest 8.0 software after staining with Coomassie blue. Valid spots were then analyzed through liquid chromatography tandem mass spectrometry (LC-MS/MS). Among the 480 total protein spots displayed in 2-D gels, 6 specific protein spots were observed in sperm gels. A search against protein sequences in the National Center for Biotechnology Information databases (NCBI) indicated that differentially expressed proteins correspond to two proteins, identified to be enolase and transcription factor AP-2-alpha (TFAP-2α). The two proteins were up-regulated in the irradiated sperm. To the best of our knowledge, this study is the first to identify proteomic changes induced by carbon ion radiation in sheep sperm. The analysis of differential expression protein may be useful in identifying new breeding markers in sheep reproduction and in clarifying the mechanisms involved in irradiation or space breeding.
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Received: 26 March 2012
Accepted:
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| Fund: This work was supported by the Key Scientific Technology Research Projects of Gansu Province, China (1102NKDA022), the Major Program of Ministry of Agriculture to Cultivate New Varieties of Genetically Modified Organisms of China (2008ZX08008-003), the National Basic Research Program of China, the National Natural Science Foundation of China (2010CB834202). |
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Corresponding Authors:
Correspondence ZHAO Xing-xu, Tel: +86-931-7632482, E-mail: zhaoxx@gsau.
edu.cn; ZHANG Hong, Tel: +86-931-4969344, E-mail: zhangh@impcas.ac.cn
E-mail: zhangh@impcas.ac.cn
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| About author: HE Yu-xuan, E-mail: heyuxuan1226@126.com; LI Hong-yan, E-mail: lihyedu@163.com |
Cite this article:
HE Yu-xuan, LI Hong-yan, ZHANG Yong, HE Jian-hua, ZHANG Hong, ZHAO Xing-xu.
2013.
Differential Proteomic Analysis of Carbon Ion Radiation in Sheep Sperm. Journal of Integrative Agriculture, 12(9): 1629-1637.
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| [1]Andre F, Jean V, Genevieve G, Doucher D. 2002. Enolaseisoforms activities in spermatozoa from mennormospermia and abnormospermia. Journal ofAndrology, 23, 202-210[2]Bartosz P M M. 2006. Large-scale and high-confidenceproteomic analysis of human seminal plasma. GenomeBiology, 7, R40.Bradford M M A. 1976. Rapid and sensitive method for thequantitation of microgram quantities of protein utilizingthe principle of protein-dye binding. AnalyticalBiochemistry, 72, 248-254[3]Braunstein S, Badura B L, Xi Q R, Formenti S C, SchneiderR J. 2009. Regulation of protein synthesis by ionizingradiation. Molecular and Cellular Biology, 29, 5645-5656[4]Breakely E A, Kronenberg A. 1998. Heavy-ion radiobiology:new approaches to delineate mechanisms underlyingenhanced biological effectiveness. RadiationResearch, 150, S126-S145.Cao W L, Wang Y X, Xiang Z Q, Li Z. 2003.Cryopreservation-induced decrease in heat-shockprotein 90 in human spermatozoa and its mechanism.Asian Journal of Andrology, 5, 43-46[5]Chen Y Y, Lin S Y, Yeh Y Y, Hsiao H H, Wu C Y, Chen S T, Wang A H. 2005. A modified protein precipitationprocedure for efficient removal of albumin from serum.Electrophoresis, 26, 2117-2127[6]Choi H J, Chung T W, Kim S J, Cho S Y, Lee Y S, Lee Y C,Ko J H, Kim C H. 2008. The AP-2alpha transcriptionfactor is required for the ganglioside GM3-stimulatedtranscriptional regulation of a PTEN gene[7]Glycobiology, 18, 395-407[8]Daòová D, Kafka I, Kalenièová Z, Nováková J. 2010. Theeffect of low dose ionizing radiation and cadmiumchloride on glucose metabolism in broiler chickens. ActaVeterinaria Brno, 79, 415-418[9]Distel L, Schwotzer G, Schussler H. 2006. Radiation-inducedDNA double-strand breaks in dependence on proteinconcentration and under aerobic and anaerobicconditions. Radiation Physics and Chemistry, 75, 210-217[10]Gitlitsa V M, Toha B H, Loveland K L, Sentry J W. 2000.The glycolytic enzyme enolase is present in sperm tailand displays nucleotide-dependent association withmicrotubules. European Journal of Cell Biology, 79,104-111[11]Guijun D, Weidong P, Gongshe L. 2006. The analysis ofproteome changes in sunflower seeds induced by N+i m p l a n t a t i o n . J o u r n a l o f B i o s c i e n c e a n dBioengineering, 31, 247-253[12]Hafez B. 2000. Reproduction in Farm Animals. 7th ed.Wiley-Blackwell, USA. p. 385.Jameel T. 2008. Sperm swim-up: a simple and effectivetechnique of semen processing for intrauterineinsemination. Journal of Pakistan MedicalAssociation, 58, 71-74[13]Janetta F, Stumpb R, Burgerb D R. 2009. Thuna suppressionof testicular function and sexual behavior byvaccinationagainst GnRH (EquityTM) in the adult stallion. AnimalReproduction Science, 115, 88-102[14]Kirkland P A, Busby J, Stevens S J, Maupin-Furlow J A.2006. Trizol-based method for sample preparation andisoelectric focusing of halophilic proteins. AnalyticalBiochemistry, 351, 254-259[15]Lee R, Nasonova E, Riter S. 2005. Chromosome aberrationyields and apoptosis in human lymphocytes irradiatedwith Fe-ions of differing LET. Advances in SpaceResearch, 35, 268-275[16]Li H Y, Zhao X X, Zhang H, Wang Y L, He Y X, Li F D, MaY J, Zhang Y. 2011. Comparative proteomics analysisof sheep sperm under two doses of heavy ion toirradiation. Journal of Nuclear Agricultural Sciences,3, 609-615[17](in Chinese)Li H Y, Zhao X X, Zhang Y, He Y X, Yang Y X. 2011.Analysis of the differential proteomics betweenfreshand frozen-thawed sheep sperm. ScientiaAgricultura Sinica, 24, 5092-5099[18](in Chinese)Liu T Y, Ma Y, Xue S, Shi J. 2012. Modifications of structureand physicochemical properties of maize starch by γ-irradiation treatments. 2012. LWT - Food Science andTechnology, 46, 156-163[19]Miarelli M, Signorelli F. 2010. Differential expression ofliver proteins in Chianina and Holstein young bulls.Journal of Animal Science, 88, 593-598[20]Müller F U, Loser K K, Kleideiter U, Neumann J, WallbrunnC, Dobner T, Scheld H H, Bantel H, Engels I H, Schulze-Osthoff K, et al. 2004. Transcription factor AP-2alphatriggers apoptosis in cardiac myocytes Cell Death andDifferentiation, 11, 485-493[21]Murakami M, Minamihisamatsu M, Sato K, Hayata I. 2001.Structural analysis of heavy ion radiation-inducedchromosome aberrations by atomic force microscopy.Journal of Biochemical and Biophysical Methods, 48,293-301[22]Neuhoff V, Arold N, Taube D, Ehrhardt W. 1988. Improvedstaining of proteins in polyacrylamide gels includingisoelectric focusing gels with clear background atnanogram sensitivity using Coomassie brilliant blue G-250 and R-250 Electrophoresis, 9, 255-262[23]Nyormoi O, Wang Z, Doan D, Ruiz M, McConkey D, Bar-Eli M. 2001. Transcription factor AP-2alpha ispreferentially cleaved by caspase 6 and degraded byproteasome during tumor necrosis factor alpha-inducedapoptosis in breast cancer cells Molecular CellBiology, 21, 4856-4867[24]Pancholi V. 2001. Multifunctional alpha-enolase: its role indiseases. Cellular and Molecular Life Sciences, 58,902-920[25]Pathak R, Sarma A, Sengupta B, Dey S K, Khuda-Bukhsh AR. 2007. Cell killing, nuclear damage and apoptosis inChinese Hamster V79 cells after irradiation with heavyionbeams of 16O, 12C and 7Li. Mutation Research, 632,58-68[26]Peddinti D, Nanduri B, Kaya A, Feugang J M, Burgess S C,Memili E. 2008. Comprehensive proteomic analysis ofbovine spermatozoa of varying fertility rates andidentification of biomarkers associated with fertility.BMC Systems Biology, 2, 1-14[27]Ramalho-Santos J, Amaral A, Sousa A P. 2007. Probing thestructure and function of mammalian sperm usingoptical and fluorescence microscopy. Modern Researchand Educational Topics in Microscopy, 1, 394-402[28]Reinhardt K, Wong C H, Georgiou A S. 2009. Detection ofseminal fluid proteins in the bed bug, cimex lectularius,using two-dimensional gel electrophoresis and massspectrometry. Parasitology, 136, 283-292[29]Su Y, Meador J A, Geard C R, Balajee A S. 2009. Analysis ofionizing radiation-induced DNA damage and repair inthree-dimensional human skin model system. Clinicaland Experimental Dermatology, 7, 1-7[30]Triwulanningsih E, Situmorang P, Sugiarti T, Sianturi R G,Kusumaningrum D A. 2010. Effect of glutathioneaddition to the sperm diluent medium on quality ofbovine chilled semen. Indonesian Journal ofAgriculture, 3, 60-65[31]Verde S C, Tenreirob R, Botelho M L. 2004. Sanitation of chicken eggs by ionizing radiation: HACCP andinactivation studies. Radiation Physics and Chemistry,71, 27-31[32]Wang J, Yu Y, Tian X J. 2012. Effect of γ-ray irradiation onthe germinating characteristics of wheat seed.Radiation Physics and Chemistry, 81, 463-465[33]Yang Y X, Zhao X X, Zhang Y. 2009. Proteomic analysis ofmammary tissues from healthy cows and clinical mastiticcows for identification of disease-related proteins.Veterinary Research Communications, 33, 295-303[34]Zhang H, Wei Z Q, Li W J, Li Q, Dang B R, Chen W Q, XieH M, Zhang S M, He J, Huang T, et al. 1999. Effects of16O+6 ion irradiation on human sperm spontaneouschemiluminescence, motility, acrosome reaction andviability in vitro. Acta Biologiae ExperimentalisSinica, 32, 1-6[35]Zhang H, Xie Y, Zhou Q M, Liu B, Li W J, Li X D, Duan X,Yuan Z G, Zhou G M, Min F L. 2006. Adaptive hormeticresponse of pre-exposure of mouse brain with low-dose12C6+ ion or 60Coc-ray on growth hormone (GH) andbody weight induced by subsequent high-doseirradiation. Advances in Space Research, 38, 1148-1151[36]Zhang J, Brewer B, Huang J, Williams T. 2000.Overexpression of transcription factor AP-2alphasuppresses mammary gland growth and morphogenesis[37]Developmental Biology, 256, 127-145[38]Zhao F, Satoda M, Licht J D, Hayashizaki Y, Gelb B D.2001. Cloning and characterization of a novel mouseAP-2 transcription factor, AP-2delta, with unique DNAbinding and transactivation properties The Journal ofBiological Chemistry, 276, 40755-4076 |
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