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| Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat |
| BAI Jian-hui, LIU Jing-hui, ZHANG Na, YANG Jun-heng, SA Ru-la , WU Lan |
1.Science Innovation Team of Oat, Inner Mongolia Agricultural University, Hohoot 010019, P.R.China
2.College of Foreign Languages, Inner Mongolia Agricultural University, Hohoot 010019, P.R.China |
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摘要 Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were: (i) low concentrations of alkali stress (25 and 50 mmol L-1) significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde (MDA) content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase (SOD), and peroxidase (POD) activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; (ii) soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; (iii) alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; (iv) under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress.
Abstract Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were: (i) low concentrations of alkali stress (25 and 50 mmol L-1) significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde (MDA) content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase (SOD), and peroxidase (POD) activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; (ii) soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; (iii) alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; (iv) under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress.
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Received: 30 July 2012
Accepted:
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| Fund: This research was supported by the National Natural Science Foundation of China (31060174, 30660084), the Natural Science Foundation of Inner Mongolia, China (2010ZD07, 200607010301), the Technology System of Agricultural Industry of China (CARS-08-B-5), and the Item of Science Innovation Team of Inner Mongolia Agricultural University (NDTD2010-8). We thank Dr. Yan Weikai in Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food in Canada and Dr. Xu Jin in the Agricultural Resource Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, for their useful suggestions on this paper. |
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Corresponding Authors:
Correspondence LIU Jing-hui, Mobile: 13848150459, E-mail: cauljh@163.com
E-mail: cauljh@163.com
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| About author: BAI Jian-hui, E-mail: bzmayyb@163.com |
Cite this article:
BAI Jian-hui, LIU Jing-hui, ZHANG Na, YANG Jun-heng, SA Ru-la , WU Lan.
2013.
Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat. Journal of Integrative Agriculture, 12(8): 1441-1449.
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| [1]Adriano S, Angelo C T, Bartolomeo D, Cristos X. 2005.Infiuence of water deficit and rewatering on thecomponents of the ascorbate-glutathione cycle in fourinterspecific Prunus hybrids. Plant Science, 169, 403-412[2]Amanda J A, Mark W S, Guest D I. 2003. Production of reactiveoxygen species during non-specific elicitation, non-hostresistance and field resistance expression in culturedtobacco cells. Functional Plant Biology, 30, 91-99[3]Ana S C, Francisco P A, Manuel C, Manuel A. 1998.Polyamines as short-term salt tolerance traits in tomato.Plant Sciencce, 138, 9-16[4]Asish P, Anath B D, Premananda D. 2002. NaCl stress causeschanges in photosynthetic pigments, proteins and othermetabolic components in the leaves of a true mangrove.Bruguiera parviflora, in hydroponic cultures. Journaland Life Sciences, 45, 28-36[5]Avinash M, Bhavanath J. 2011. Antioxidant response ofthe microalga Dunaliella salina under salt stress.Botanica Marina, 54, 195-199[6]Bai B Z, Shi A G, Zhang J Y. 2001. Plant Physiology. BeijingAgricultural Press, China. pp. 6-61[7](in Chinese)Bartels D. 2001. Targeting detoxification pathways: anefficient approach to obtain plants with multiple stresstolerance. Trends in Plant Science, 6, 284-286[8]Brand J D, Tang C, Rathjen A J. 2002. Screening roughseededlupins (Lupinus pilosus murr. and Lupinusatlanticus glads.) for tolerance to calcareous soils.Plant and Soil, 245, 261-275[9]Cavalcanti F R, Santos-Lima J P M, Ferreira-Silva S L, Viegas RA, Silveira J A G. 2007. Roots and leaves display contrastingoxidative response during salt stress and recovery incowpea. Journal of Plant Physiology, 164, 591-600[10]Foyer C H, Descourvieres P, Kunert K J. 1994. Protectionagainst oxygen radicals: an important defensemechanism studied in transgenic plants. Plant CellEnvironment, 17, 507-523[11]Fu G F, Song J, Li Y R, Yue M K, Xiong J, Tao L X. 2010.Alterations of panicle antioxidant metabolism andcarbohydrate content and pistil water potential involvedin spikelet sterility in rice under water-deficit stress.Rice Science, 17, 303-310[12]Gao C, Wang Y C, Liu G F, Yang C P, Jiang J, Li H Y. 2008.Expression profiling of salinity-alkali stress responsesby large-scale expressed sequence tag analysis inTamarix hispid. Plant Molecular Biology, 66, 245-258[13]Khosravinejad F, Heydari R, Farboodnia Y. 2008.Antioxidant responses of two barley varieties to salinestress. Pakistan Journal of Biological Science, 11,905-909[14]Koch K. 2004. Sucrose metabolism: regulatory mechanismsand pivotal roles in sugar sensing and plantdevelopment. Current Opinion in Plant Biology, 7,235-246[15]Lehner B. 2008. Selection to minimise noise in livingsystems and its implications for the evolution of geneexpression. Molecular Systems Biology, 4, 170-176[16]Li X Y, Liu J J, Zhang Y T, Lin J X, Mu C S. 2011.Physiological responses and adaptive strategies ofwheat seedlings to salt and alkali stresses. Soil Scienceand Plant Nutrition, 55, 680-684[17]Liang C, Zhang X Y, Luo Y, Wang G P, Zhou Q, Wang W.2009. Overaccumulation of glycine betaine alleviatesthe negative effects of salt stress in wheat. RussianJournal of Plant Physiology, 56, 370-376[18]Liao Y, Chen G Z. 2007. Physiological adaptability of threemangrove species to salt stress. Acta Ecologica Sinica,27, 2208-2214[19]Liu J, Guo W Q, Shi D C. 2010. Seed germination, seedlingsurvival, and physiological response of sunflowersunder saline and alkaline conditions. Photosynthetica,48, 278-286[20]Liu Y P, Ji Q L, Zhou X Y, Ge C H, Zhang F C. 2006. Effect ofM1-M2 generation oat seedlings on salt-tolerance afterimplanted by N+ ion beams. Biotechnology, 16, 73-76[21]Mariana R, Carolina P, Griselda P, Roque I, Juan A G, MirnaH, Fernando E P. 2009. Soluble sugars-metabolism,sensing and abiotic stress. Plant Signaling &Behavior, 4, 388-393[22]Meenakshi G, Bavita A. 2010. Polyamine catabolisminfluences antioxidative defense mechanism in shootsand roots of five wheat genotypes under hightemperature stress. Plant Growth Regulation, 60, 13-25[23]Mutlu S, Atici Ö, Nalbantoglu B. 2009. Effects of salicylicacid and salinity on apoplastic antioxidant enzymes intwo wheat cultivars differing in salt tolerance. BiologiaPlantarum, 53, 334-338[24]Nguyen G N, Hailstones D L, Wilkes M, Sutton B G. 2010.Role of carbohydrate metabolism in drought-inducedmale sterility in rice anthers. Journal of Agronomy andCrop Science, 196, 346-357[25]Rana M, Mark T. 2008. Mechanisms of salinity tolerance.Annual Review of Plant Biology, 59, 651-681[26]Rizhsky L, Liang H, Shuman J, Shulaev V, Davletova S,Mittler R. 2004. When defense pathways collide. Theresponse of Arabidopsis to a combination of droughtand heat stress. Plant Physiology, 134, 1683-1696[27]Sheoran I S, Saini H S. 1996. Drought-induced male sterility inrice: changes in carbohydrate levels and enzyme activitiesassociated with the inhibition of starch accumulation inpollen. Sexual Plant Reproduction, 9, 161-169[28]Thomas R. 1999. Source-sink regulation by sugar andstress. Current Opinion in Plant Biology, 2, 198-206[29]Wang C Q, Guo Y M, Li B. 2008. Effects of cd stress on thecontent of MDA in leaves of the hybrid ric and their parents.Acta Ecologica Sinica, 28, 5377-5384[30](in Chinese)Wu J Y, Liu J H, Li Q, Fu Z J. 2009. Effect of salt stress onoat seed germination and seeding membranepermeability. Journal of Triticeae Crops, 29, 341-345[31](in Chinese)Yan Y Q, Wang W J, Zhu H, Shi X C, Liu X L, Zu Y G. 2009.Effects of salt-alkali stress on osmoregulation substanceand active oxygen metabolism of qingshan poplar(Populus pseudo-cathayana × P. deltoides). ChineseJournal of Applied Ecology, 20, 2085-2091 (in Chinese)[32]Yang C W, Shi D C, Wang D L. 2008. Comparative effects ofsalt and alkali stresses on growth, osmotic adjustmentand ionic balance of an alkali-resistant halophyte Suaedaglauca (Bge.). Plant Growth Regulation, 56, 179-190[33]Yang C W, Wang P, Li C Y, Shi D C, Wang D L. 2008.Comparison of effects of salt and alkali stresses on thegrowth and photosynthesis of wheat. Photosynthetica,46, 107-114[34]Yang C W, Xu H H, Wang L L, Liu J, Shi D C, Wang D L. 2009.Comparative effects of salt-stress and alkali-stress onthe growth, photosynthesis, solute accumulation, andion balance of barley plants. Photosynthetica, 47, 79-86[35]Yang K, Zhang B J, Hu Y G, Wang S H, Que X F. 2009.Effects of complex saline alkaline stress on seedsgermination and physiological and biochemicalparameters of oats seedlings. Agricultural Researchin the Arid Areas, 27, 188-192 (in Chinese)[36]Yemm E W, Willis A J. 1954. The estimation of carbohydratesin plant extracts by anthrone. Biochemical Journal,57, 508-514[37]Zhao H X. 2010. The effect of alkali on SOD, POD activity andMDA contents of rice at seedling stage. HeilongjiangAgricultural Sciences, 8, 22-23 (in Chinese)[38]Zhang D P, Cao B H, Jia B, Tang Q. 2008. Germination andphysiological response of Albizia julibrissin seeds underalkali-salt stress. Scientia Silvae Sinicae, 44, 157-161[39]Zheng Y H, Xu X B, Wang M Y, Zheng X H, Li Z J, Jiang JM. 2009. Responses of salt-tolerant and intolerant wheatgenotypes to sodium chloride: photosynthesis,antioxidants activities, and yield. Photosynthetica, 47,87-94[40]Zhou J, Zhang L, Yuan D Y, Qi A G. 2008. Determination ofphysiological indices in Albizzia julibrissin Durazzseedlings under alkaline stress with visiblespectrophotometry. Spectroscopy and SpectralAnalysis, 28, 418-421. (in Chinese) |
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