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Cytochemical localization of H2O2 in pigment glands of cotton (Gossypium hirsutum L.) |
WANG Ling-li1, 2, ZHENG Shuang-shuang1, TONG Pan-pan1, CHEN Yan1, LIU Wen-zhe1 |
1 School of Life Science, Northwest University, Xi’an 710069, P.R.China
2 Department of Life Science, Yuncheng University, Yuncheng 044000, P.R.China |
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Abstract Programmed cell death (PCD) plays a critical role in the development of plant pigment glands, while H2O2, which is a kind of reactive oxygen species (ROS) produced by the aerobic metabolism of cells, acts as an important signal in this process. Here, we investigated the temporal and spatial dynamics of accumulated H2O2 in pigment glands of Gossypium hirsutum L. with 3,3-diaminobenzidine (DAB) staining, 2’,7’-dichlorodihydrofluorescein diacetate (DCFH2)-DA fluorescent labeling and CeCl3 cytochemical localization techniques. The results showed that the pigment glands of G. hirsutum could generate H2O2, and the amount and localization of H2O2 varied at different developmental stages. At the early developmental stage, a small amount of HH2O2 accumulated in the vacuole membrane of pigment gland cells. At the intermediate stage, a large number of H2O2 appeared in the vacuole membrane, while cell walls started to accumulate a small amount of H2O2. When pigment gland cell degraded, H2O2 mainly accumulated on the chloroplast envelope membrane of inner sheath cells. With the degradation of the sheath cells, H2O2 was detected in cell wall and the membrane of secretory vesicles which contains the preliminary contents of pigment gland. With the pigment glands completely maturation, H2O2 would disappeared. The accumulation sites of H2O2 are consistent with the process of PCD of individual gland cells, which started from the degradation of intracellular membrane and ended with the degradation of cell walls. Thus H2O2 probably plays an important role in the development of pigment glands. In addition, the development of pigment glands and the generation of H2O2 are not associated with the light, and no H2O2 was detected in the secretions of pigment glands.
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Received: 20 July 2015
Accepted:
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Fund: This study was supported by the National Natural Science Foundation of China (31270428). |
Corresponding Authors:
LIU Wen-zhe, Tel: +86-29-88302184,
Fax: +86-29-8830357, E-mail: lwenzhe@nwu.edu.cn
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About author: WANG Ling-li, E-mail: wanglingli_521@163.com |
Cite this article:
WANG Ling-li, ZHENG Shuang-shuang, TONG Pan-pan, CHEN Yan, LIU Wen-zhe.
2016.
Cytochemical localization of H2O2 in pigment glands of cotton (Gossypium hirsutum L.). Journal of Integrative Agriculture, 15(7): 1490-1498.
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Apel K, Hirt H. 2004. Reactive oxygen species: Metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology, 55, 373–399.Bais H P, Vepachedu R, Gilroy S, Callaway R M, Vivanco J M. 2003. Allelopathy andexotic plant invasion: From molecules and genes to species interactions. Science, 301, 1377–1380.Bell A A, Stipanovic R D. 1977. The chemical composition, biological activity, and genetics of pigment glands in cotton. In: Proceedings of the Beltwide Cotton Conferences, 10–12 January 1977, Atlanta, GA. Cotton Foundation Publisher, Memphis, TN. pp. 244–258.Bestwick C S, Brown I R, Bennett M H, Mansfield J W. 1997. Localization of hydrogen peroxide accumulation during the hypersensitive reaction of lettuce cells to Pseudomonas syringae pv phaseolicola. The Plant Cell, 9, 209–221. Bethke P C, Jones R L. 2001. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species. The Plant Journal, 25,19–29.Bienert G P, Schjoerring J K, Jahn T P. 2006. Membrane transport of hydrogen peroxide. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1758, 994–1003.Clement M V, Pervaiz S. 2001. Intracellular superoxide and hydrogen peroxide concentrations: A critical balance that determines survival or death. Redox Report, 6, 211–214.Dat J, Vandenabeele S, Vranová E, Van Montagu M, Inzé D, Van Breusegem F. 2000. Dual action of the active oxygen species during plant stress responses. Cellular and Molecular Life Sciences, 57, 779–795. Doyle S M, Diamond M, McCabe P F. 2010. Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures. Journal of Experimental Botany, 61, 473–482. Fath A, Bethke P C, Lonsdale J, Meza-Romero R, Jones R L. 2000. Programmed cell death in cereal aleurone. Plant Molecular Biology, 44, 255–266.Fath A, Bethke P C, Jones R L. 2001. Enzymes that scavenge reactive oxygen species are down-regulated prior to gibberellic acid-induced programmed cell death in barley aleurone. Plant Physiology, 126, 156–166.Gadjev I, Stone J M, Gechev T S. 2008. Programmed cell death in plants: New insights into redox regulation and the role of hydrogen peroxide. International Review of Cell and Molecular Biology, 270, 87–144.Gechev T S, Gadjev I,Van Breusegem F, Inzé D, Dukiandjiev S, Toneva V, Minkov I. 2002. Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cellular and Molecular Life Sciences, 59, 708–714.Gechev T S, Minkov I N, Hille J. 2005. Hydrogen peroxide-induced cell death in Arabidopsis: Tanscriptional and mutant analysis reveals a role of an oxoglutarate-dependent dioxygenase gene in the cell death process. IUBMB Life, 57, 181–188.Gechev T S, Van Breusegem F, Stone J M, Denev I, Laloi C. 2006. Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays, 28, 1091–1101. Heath M C. 2000. Nonhost resistance and nonspecific plant defenses. Current Opinion in Plant Biology, 3, 315–319.Henzler T, Steudle E. 2000. Transport and metabolic degradation of hydrogen peroxide in Chara corallina: Model calculations and measurements with the pressure probe suggest transport of H2O2 across water channels. Journal of Experimental Botany, 51, 2053–2066.Hu D Y. 2002. Studies on the structures and utilization in cotton breeding for the pigment gland. MSc thesis, Zhejiang University, China. (in Chinese) Liu W Z, Zhou Y F, Wang X, Jiao Z J. 2010. Programmed cell death during pigment gland formation in Gossypium hirsutum leaves. Plant Biology, 12, 895–902.Metcalfe C R, Chalk L. 1957. Anatomy of the Dicotyledons. Clarendon Press, Oxford. p. 226. Montillet J L, Chamnongpol S, Rustérucci C, Dat J, van de Cotte B, Agnel J P, Battesti C, Inzé D, Van Breusegem F, Triantaphylidès C. 2005. Fatty acid hydroperoxides and H2O2 in the execution of hypersensitive cell death in tobacco leaves. Plant Physiology, 138, 1516–1526.Mueller M J. 2004. Archetype signals in plants: The phytoprostanes. Current Opinion in Plant Biology, 7, 441–448.Ni X L, Meng Y, Zheng S S, Liu W Z. 2014. Programmed cell death during aerenchyma formation in Typha angustifolia leaves. Aquatic Botany, 113, 8–18.Orozco-Cárdenas M L, Narváez-Vásquez J, Ryan C A. 2001. Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. The Plant Cell, 13, 179–192.Orozco-Cárdenas M L, Ryan C A. 1999. Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway. Proceedings of the National Academy of Sciences of the United States of America, 96, 6553–6557.Pervaiz S, Clement M V. 2007. Superoxide anion: Oncogenic reactive oxygen species? International Journal of Biochemistry & Cell Biology, 39, 1297–1304.Sagi M, Fluhr R. 2001. Superoxide production by plant homologues of the gp91phox NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection. Plant Physiology, 126, 1281–1290.Sagi M, Fluhr R. 2006. Production of reactive oxygen species by plant NADPH oxidases. Plant Physiology, 141, 336–340.Steffens B, Sauter M. 2005. Epidermal cell death in rice (Oryza sativa L.) is regulated by ethylene, gibberellin and abscisic acid. Plant Physiology, 139, 713–721. Steffens B, Sauter M. 2009. Epidermal cell death in rice is confined to cells with a distinct molecular identity and is mediated by ethylene and H2O2 through an autoamplified signal pathway. The Plant Cell, 21, 184–196.Thordal-Christensen H, Zhang Z G, Wei Y D, Collinge D B. 1997. Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley-powdery mildew interaction. The Plant Journal, 11, 1187–1194.Torres M A. 2010. ROS in biotic interactions. Physiologia Plantarum, 138, 414–429.Torres M A, Dangl J L, Jones J D. 2002. Arabidopsis gp91phox homologues AtrbohD and AtrbohF are required for accumulation of reactive oxygen intermediates in the plant defense response. Proceedings of the National Academy of Sciences of the United States of America, 99, 517–522.Wendel J F, Brubaker C L, Seelanan T. 2010. The origin and evolution of Gossypium. In: Physiology of Cotton. Springer Netherland. pp. 1–18.Yang C Q, Wu X M, Ruan J X, Hu W L, Mao Y B, Chen X Y, Wang L J. 2013. Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum). Phytochemistry, 96, 46–56.Zafra A, Rodríguez-García M I, Alché Jde D. 2010. Cellular localization of ROS and NO in olive reproductive tissues during flower development. BMC Plant Biology, 24, 36–49.Zhou Y F, Mao S L, Li S F, Ni X L, Li B, Liu W Z. 2014. Programmed cell death: A mechanism for the lysigenous formation of secretory cavities in leaves of Dictamnus dasycarpus. Plant Science, 225, 147–160.Zhu S J, Ji D F, Liu S A, Wang R H. 2001. The effects of cotton pigment gland and gossypol on the growth and insecticide resistance of cotton bollworm, Helicoverpa armigera (Hübner). Scientia Agricultura Sinica, 34, 157–162. (in Chinese)Zurbriggen M D, Carrillo N, Hajirezaei M R. 2010. ROS signaling in the hypersensitive response: When, where and what for? Plant Signaling and Behaviour, 5, 393–396. |
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