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Journal of Integrative Agriculture  2014, Vol. 13 Issue (5): 1082-1089    DOI: 10.1016/S2095-3119(13)60313-5
Animal Science · Veterinary Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine
 XIAO Sui, FEI Chen-zhong, ZHANG Li-fang, ZHENG Wen-li, ZHANG Ke-yu , XUE Fei-qun
Key Laboratory of Veterinary Drug Safety Evaluation and Residues Research, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, P.R.China
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摘要  Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography (HPLC) fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharmacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.

Abstract  Quality control and screening of active substances in traditional Chinese medicines have been performed using fingerprint analysis. The spectrum-effect relationship between chromatography fingerprints and efficacy of herbal drugs is considered as a potentially useful method for determining active ingredients in complex mixtures. The study was designed to develop a method for determining the bioactive components of a compound Chinese medicine called Tiefeng based on spectrum-effect relationships between high-performance liquid chromatography (HPLC) fingerprints and anticoccidial activities. Four peaks of the established HPLC fingerprint indicate the main bioactive components of this medicine. In addition, pharmacodynamic atlas was defined and used to assess the anticoccidial activity of Tiefeng from different sources for the first time. We found that the level of anticoccidial activity of Tiefeng was consistent with the degree of similarity between the pharmacodynamic atlas and chromatogram of any sample. Furthermore, effect of this medicine was related with the main active constituents, along with the origin and the harvesting time.
Keywords:  herb medicine       fingerprint       anticoccidial index       spectrum-effect relationship  
Received: 27 December 2012   Accepted:
Fund: 

The authors would like to acknowledge the Key Laboratory of Animal Parasitology of the Ministry of Agriculture for providing the laboratory equipments used in this study. This work was funded by the National Key Technology R&D Program, China (2008 BADB4B05-02).

Corresponding Authors:  XUE Fei-qun, Tel: +86-21-34293460, Fax: +86-21-34293396, E-mail: xuefeiqun@yahoo.cn, feiqun@gmail.com     E-mail:  xuefeiqun@yahoo.cn, feiqun@gmail.com

Cite this article: 

XIAO Sui, FEI Chen-zhong, ZHANG Li-fang, ZHENG Wen-li, ZHANG Ke-yu , XUE Fei-qun. 2014. Spectrum-Effect Relationship Between High Performance Liquid Chromatography Fingerprints and Anticoccidial Activities of a Compound Chinese Medicine. Journal of Integrative Agriculture, 13(5): 1082-1089.

Awais M M, Akhtar M, Muhammad F, Anwar M I. 2011. Immunotherapeutic effects of some sugar cane (Saccharum officinarum L.) extracts against coccidiosis in industrial broiler chickens. Experimental Parasitology, 128, 104-110

 Cao G, Zhang Y, Feng J, Cai H, Zhang C, Ding M, Cong X, Cai B. 2011. A rapid and sensitive assay for determining the main components in processed fructus corni by UPLC-Q-TOF-MS. Chromatographia, 73, 135-141

 Chen C, Zhang H, Xiao W, Yong Z P, Bai N. 2007. High- performance liquid chromatographic fingerprint analysis for different origins of sea buckthorn berries. Journal of Chromatography (A), 1154, 250-259

 Chen Y, Yan Y, Xie M Y, Nie S P, Liu W, Gong X F, Wang Y X. 2008. Development of a chromatographic fingerprint for the chloroform extracts of Ganoderma l u c i d u m b y HPLC and LC-MS. J o u r n a l of Pharmaceutical and Biomedical Analysis, 47, 469-477

 Han C, Shen Y, Chen J, Lee F S C, Wang X. 2008. HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root. Journal of Chromatography B, 862, 125-131

 Johnson J, Reid W M. 1970. Anticoccidial drugs: Lesion scoring techniques in battery and floor-pen experiments with chickens. Experimental Parasitology, 28, 30-36

 Kannel P R, Lee S, Kanel S R, Khan S P. 2007. Chemometric application in classification and assessment of monitoring locations of an urban river system. Analytica Chimica Acta, 582, 390-399

 K o d a m a Y, Yokoyam A H, Nguyen S V. 2004. Compositions Against Chicken Coccidiosis. WO Patent. WO 2004002527 A1, 2004-01-08

 Kong W J, Zhao Y L, Shan L M, Xiao X H, Guo W Y. 2008. Investigation on the spectrum-effect relationships of EtOAc extract from Radix isatidis based on HPLC fingerprints and microcalorimetry. Journal of Chromatography (B), 871, 109-114

 Kong W J, Zhao Y L, Xiao X H, Wang J B, Li H B, Li Z L, Jin C, Liu Y. 2009. Spectrum-effect relationships between ultra performance liquid chromatography fingerprints and anti-bacterial activities of Rhizoma coptidis. Analytica Chimica Acta, 634, 279-285

 Liang X, Jin Y, Wang Y, Jin G, Fu Q, Xiao Y. 2009. Qualitative and quantitative analysis in quality control of traditional Chinese medicines. Journal of Chromatography (A), 1216, 2033-2044

 Liang Y Z, Xie P, Chan K. 2004. Quality control of herbal medicines. Journal of Chromatography (B), 812, 53-70

 Ma D, Ma C, Pan L, Li G, Yang J, Hong J, Cai H, Ren X. 2011.Vaccination of chickens with DNA vaccine encoding Eimeria acervulina 3-1E and chicken IL- 15 offers protection against homologous challenge

 Experimental Parasitology, 127, 208-214

 Peek H, Landman W. 2003. Resistance to anticoccidial drugs of Dutch avian Eimeria spp. field isolates originating from 1996, 1999 and 2001. Avian Pathology, 32, 391-401

 Shah M A, Song X, Xu L, Yan R, Song H, Ruirui Z, Chengyu L, Li X. 2010. The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis. Parasitology Research, 107, 747-750

 Sun L Q, Ding X P, Qi J, Yu H, He S, Zhang J, Ge H X, Yu B Y. 2012. Antioxidant anthocyanins screening through spectrum-effect relationships and DPPH-HPLC- DAD analysis on nine cultivars of introduced rabbiteye blueberry in China. Food Chemistry, 132, 759-765

 Wang L, Pan J, Yang M, Wu J, Yang J. 2011. Chromatographic fingerprint analysis and simultaneous determination of eight lignans in Justicia procumbens and its compound preparation by HPLC-DAD. Journal of Separation Science, 34, 667-674

 Xu X, Jiang J, Liang Y, Li X, Yi L, Cheng J. 2011. Chromatographic fingerprint analysis of Fructus Aurantii Immaturus by HPLC-DAD and chemometric methods. Journal of Central South University of Technology, 18, 353-360

 Xue F, Zhang L, Fei C, Di L, Qiu M, Zhao Q. 2007. A Compound Chinese Medicine of Prevention and Control of Coccidiosis. ZL 03150699.2 (in Chinese)

Yang L, Wu D, Tang X, Peng W, Wang X, Ma Y, Su W. 2005. Fingerprint quality control of Tianjihuang by high-performance liquid chromatography-photodiode array detection. Journal of Chromatography (A), 1070, 35-42

 Yi L, Yuan D, Liang Y, Xie P, Zhao Y. 2007. Quality control and discrimination of Pericarpium Citri Reticulatae and Pericarpium Citri Reticulatae Viride based on high-performance liquid chromatographic fingerprints and multivariate statistical analysis. Analytica Chimica Acta, 588, 207-215

 Zhou B, Wang H, Wang X, Zhang L, Zhang K, Xue F. 2010. Eimeria tenella: Effects of diclazuril treatment on microneme genes expression in second-generation merozoites and pathological changes of caeca in parasitized chickens. Experimental Parasitology, 125, 264-270
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