|
|
|
|
|
| Differential Responses of Nitrifier and Denitrifier to Dicyandiamide in Short- and Long-Term Intensive Vegetable Cultivation Soils |
| LIU Yi, YANG Yang, QIN Hong-ling, ZHU Yi-jun , WEI Wen-xue |
1、Key Laboratory of Agro-Ecological Processes in Subtropical Regions and Taoyuan Station of Agro-Ecology Research, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan 410125, P.R.China
2、Tianjin Land & Resources & Housing Management Research Centre, Tianjin 300041, P.R.China |
|
|
|
摘要 Nitrification inhibitors, such as dicyandiamide (DCD), have been shown to decrease leaching from urea- and ammonium- based fertilizers in agricultural soils. The effect of nitrification inhibitors on nitrifier and denitrifier in short- and long-term intensive vegetable cultivation soils was poorly understood. In this study, the pot trial was conducted to investigate the differential responses of nitrifier (amoA-containing bacteria) and denitrifier (nirK-containing bacteria) to DCD in short-(soil S) and long-term (soil L) intensive vegetable cultivation soils. Quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were employed to detect the abundance and composition of amoA- and nirK-containing communities. The results indicated that application of DCD led to a consistently higher NH4 +-N concentration during the whole incubation in soil L, while it was quickly decreased in soil S after 21 days. Furthermore, DCD induced more severe decrease of the abundance of amoA-containing bacteria in soil L than in soil S. However, the abundance of the nirKcontaining community was not significantly affected by DCD in both soils. Long-term vegetable cultivation resulted in a super-dominant amoA-containing bacteria group and less divergence in soil L compared with soil S, and DCD did not cause obvious shifts of the composition of ammonia-oxidising bacteria (AOB). On the contrary, both amoA- and nirK-containing bacterial compositions were influenced by DCD in soil S. The results suggested that long-term intensive vegetable cultivation with heavy nitrogen fertilization resulted in significant shifts of AOB community, and this community was sensitive to DCD, but denitrifiers were not clearly affected by DCD.
Abstract Nitrification inhibitors, such as dicyandiamide (DCD), have been shown to decrease leaching from urea- and ammonium- based fertilizers in agricultural soils. The effect of nitrification inhibitors on nitrifier and denitrifier in short- and long-term intensive vegetable cultivation soils was poorly understood. In this study, the pot trial was conducted to investigate the differential responses of nitrifier (amoA-containing bacteria) and denitrifier (nirK-containing bacteria) to DCD in short-(soil S) and long-term (soil L) intensive vegetable cultivation soils. Quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were employed to detect the abundance and composition of amoA- and nirK-containing communities. The results indicated that application of DCD led to a consistently higher NH4 +-N concentration during the whole incubation in soil L, while it was quickly decreased in soil S after 21 days. Furthermore, DCD induced more severe decrease of the abundance of amoA-containing bacteria in soil L than in soil S. However, the abundance of the nirKcontaining community was not significantly affected by DCD in both soils. Long-term vegetable cultivation resulted in a super-dominant amoA-containing bacteria group and less divergence in soil L compared with soil S, and DCD did not cause obvious shifts of the composition of ammonia-oxidising bacteria (AOB). On the contrary, both amoA- and nirK-containing bacterial compositions were influenced by DCD in soil S. The results suggested that long-term intensive vegetable cultivation with heavy nitrogen fertilization resulted in significant shifts of AOB community, and this community was sensitive to DCD, but denitrifiers were not clearly affected by DCD.
|
|
Received: 10 January 2013
Accepted:
|
| Fund: This work was financially supported by the National Natural Science Foundation of China (41071181, 41090282) and the Chinese Academy of Sciences (KZCX2-YW-T07). |
|
Corresponding Authors:
WEI Wen-xue, Tel: +86-731-84615210, E-mail: wenxuewei@isa.ac.cn
E-mail: wenxuewei@isa.ac.cn
|
| About author: LIU Yi, Mobile: 13467585846, E-mail: liuyi@isa.ac.cn; |
Cite this article:
LIU Yi, YANG Yang, QIN Hong-ling, ZHU Yi-jun , WEI Wen-xue.
2014.
Differential Responses of Nitrifier and Denitrifier to Dicyandiamide in Short- and Long-Term Intensive Vegetable Cultivation Soils. Journal of Integrative Agriculture, 13(5): 1090-1098.
|
| A m b e r g e r A. 1989. Research on dicyandiamide as a nitrification inhibitor and future outlook. Communications in Soil Science and Plant Analysis, 20, 1933-1955 Ball B C, Cameron K C, Di H J, Moore S. 2012. Effects of trampling of a wet dairy pasture soil on soil porosity and on mitigation of nitrous oxide emissions by a nitrification inhibitor, dicyandiamide. Soil Use and Management, 28, 194-201 Carneiro J, Cardenas L M, Hatch D J, Trindade H, Scholefield D, Clegg C D, Hobbs P. 2010. Effect of the nitrification inhibitor dicyandiamide on microbial communities and N2O from an arable soil fertilized with ammonium sulphate. Environmental Chemistry Letters, 8, 237-246 Chen Z, Liu J B, Wu M N, Xie X L, Wu J S, Wei W X. 2012. Differentiated response of denitrifying communities to fertilization regime in paddy soil. Microbial Ecology, 63, 446-459 Chen Z, Luo X Q, Hu R G, Wu M N, Wu J S, Wei W X. 2010. Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil. Microbial Ecology, 60, 850-861 Cui M, Sun X C, Hu C X, Di H J, Tan Q L, Zhao C S. 2011. Effective mitigation of nitrate leaching and nitrous oxide emissions in intensive vegetable production systems using a nitrification inhibitor, dicyandiamide. Journal of Soils and Sediments, 11, 722-730 Dambreville C, Hallet S, Nguyen C, Morvan T, Germon J C, Philippot L. 2006. Structure and activity of the denitrifying community in a maize-cropped field fertilized with composted pig manure or ammonium nitrate. FEMS Microbiology Ecology, 56, 119-131 Di H J, Cameron K C, Shen J P, Winefield C S, O’Callaghan M, Bowatte S, He J Z. 2010. Ammonia-oxidizing bacteria and archaea grow under contrasting soil nitrogen conditions. FEMS Microbiology Ecology, 72, 386-394 Di H J, Cameron K C. 2012. How does the application of different nitrification inhibitors affect nitrous oxide emissions and nitrate leaching from cow urine in grazed pastures? Soil Use and Management, 28, 54-61 Dunbar J, Ticknor L O, Kuske C R. 2001. Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of 16S rRNA genes from bacterial communities. Applied and Environmental Microbiology, 67, 190-197 Enwall K, Philippot L, Hallin S. 2005. Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization. Applied and Environmental Microbiology, 71, 8335-8343 Hart S C, Stark J M, Davidson E A, Firestone M K. 1994. Nitrogen mineralization, immobilization, and nitrification. In: Methods of Soil Analysis. Soil Science Society of America, Madison, Wisconsin. pp. 985-1018 Henry S, Baudoin E, López-Gutiérrez J C, Martin-Laurent F, Brauman A, Philippot L. 2004. Quantification of denitrifying bacteria in soils by nirK gene targeted real-time PCR. Journal of Microbiological Methods, 59, 327- 335. Horz H P, Rotthauwe J H, Lukow T, Liesack W. 2000. Identification of major subgroups of ammonia-oxidizing bacteria in environmental samples by T-RFLP analysis of amoA PCR products. Journal of Microbiological Methods, 39, 197-204 Jackson M L. 1958. Soil Chemical Analysis. Prentice-Hall, Englewood Cliffs, NJ, USA. Kelliher F M, Clough T J, Clark H, Rys G, Sedcole J R. 2008. The temperature dependence of dicyandiamide (DCD) degradation in soils: A data synthesis. Soil Biology & Biochemistry, 40, 1878-1882 Lu R K. 1999. Analysis Methods of Soil Agricultural Chemistry. Chinese Agricultural Science and Technology Press, Beijing. pp. 105-254 (in Chinese) Mertens J, Broos K, Wakelin S A, Kowalchuk G A, Springael D, Smolders E. 2009. Bacteria, not archaea, restore nitrification in a zinc-contaminated soil. Isme Journal, 3, 916-923 Morimoto S, Hayatsu M, Hoshino Y T, Nagaoka K, Yamazaki M, Karasawa T, Takenaka M, Akiyama H. 2011. Quantitative analyses of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in fields with different soil types. Microbes and Environments, 26, 248-253 O’Callaghan M, Gerard E M, Carter P E, Lardner R, Sarathchandra U, Burch G, Ghani A, Bell N. 2010. Effect of the nitrification inhibitor dicyandiamide (DCD) on microbial communities in a pasture soil amended with bovine urine. Soil Biology and Biochemistry, 42, 1425-1436 O’Connor P J, Hennessy D, Brophy C, O’Donovan M, Lynch M B. 2012. The effect of the nitrification inhibitor dicyandiamide (DCD) on herbage production when applied at different times and rates in the autumn and winter. Agriculture, Ecosystems & Environment, 152, 79-89 Rotthauwe J H, Witzel K P, Liesack W. 1997. The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Applied and Environmental Microbiology, 63, 4704-4712 Sait L, Galic M, Strugnell R A, Janssen P H. 2003. Secretory antibodies do not affect the composition of the bacterial microbiota in the terminal ileum of 10-week-old mice. Applied and Environmental Microbiology, 69, 2100- 2109. Serna M D, Legaz F, PrimoMillo E. 1996. Improvement of the N fertilizer efficiency with dicyandiamide (DCD) in citrus trees. Fertilizer Research, 43, 137-142 Shen J P, Zhang L M, Zhu Y G, Zhang J B, He J Z. 2008. Abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea communities of an alkaline sandy loam. Environmental Microbiology, 10, 1601-1611 Shen W S, Lin X G, Shi W M, Min J, Gao N, Zhang H Y, Yin R, He X H. 2010. Higher rates of nitrogen fertilization decrease soil enzyme activities, microbial functional diversity and nitrification capacity in a Chinese polytunnel greenhouse vegetable land. Plant and Soil, 337, 137-150 Shi W M, Yao J, Yan F. 2009. Vegetable cultivation under greenhouse conditions leads to rapid accumulation of nutrients, acidification and salinity of soils and groundwater contamination in South-Eastern China. Nutrient Cycling in Agroecosystems, 83, 73-84 Soares J R, Cantarella H, Menegale M L D. 2012. Ammonia volatilization losses from surface-applied urea with urease and nitrification inhibitors. Soil Biology & Biochemistry, 52, 82-89 Wang S, Ye J, Perez P G, Huang D F. 2011. Abundance and diversity of ammonia-oxidizing bacteria in rhizosphere and bulk paddy soil under different duration of organic management. African Journal of Microbiology Research, 5, 5560-5568 Xia W W, Zhang C X, Zeng X W, Feng Y Z, Weng J H, Lin X G, Zhu J G, Xiong Z Q, Xu J, Cai Z C, Jia Z J. 2011. Autotrophic growth of nitrifying community in an agricultural soil. Isme Journal, 5, 1226-1236 Zhu J H, Li X L, Christie P, Li J L. 2005. Environmental implications of low nitrogen use efficiency in excessively fertilized hot pepper (Capsicum frutescens L.) cropping systems. Agriculture, Ecosystems & Environment, 111, 70-80 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
| |
Shared |
|
|
|
|
| |
Discussed |
|
|
|
|
