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| Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam. |
| ZHAO Ning, ZHAI Hong, YU Xiao-xia, LIU Zhe-sheng, HE Shao-zhen, LI Qiang, MA Dai-fu , LIU Qing-chang |
1.Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/China Agricultural University, Beijing 100193, P.R.China
2.Xuzhou Sweetpotato Research Center, Xuzhou 221121, P.R.China |
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摘要 Sequence-related amplification polymorphism (SRAP) markers closely linked to stem nematode resistance gene were developed in sweetpotato, Ipomoea batatas (L.) Lam. Using bulked segregant analysis (BSA), 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781×susceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nsp1, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.
Abstract Sequence-related amplification polymorphism (SRAP) markers closely linked to stem nematode resistance gene were developed in sweetpotato, Ipomoea batatas (L.) Lam. Using bulked segregant analysis (BSA), 200 SRAP primer combinations were screened with the resistant and susceptible bulked DNA from the 196 progenies of an F1 single-cross population of resistant parent Xu 781×susceptible parent Xushu 18, 77 of them showed polymorphic bands between resistant and susceptible DNA. Primer combinations detecting polymorphism between the two bulks were used to screen both parents and 10 individuals from each of the bulks. The results showed that primer combination A9B4 produced 3 specific bands in the resistant plants but not in the susceptible plants, suggesting that the markers, named Nsp1, Nsp2 and Nsp3, respectively, linked to a gene for stem nematode resistance. Primer combination A3B6 also produced a SRAP marker named Nsp4 linking to the resistance gene. Amplified analysis of the 196 F1 individuals indicated that the genetic distance between these markers and the resistance gene was 4.7, 4.7, 6.3, and 9.6 cM, respectively.
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Received: 16 August 2011
Accepted:
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| Fund: This work was supported by China Agriculture Research System (CARS-11, Sweetpotato) and the National 863 Program of China (2012AA101204). |
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Corresponding Authors:
Correspondence LIU Qing-chang, Tel/Fax: +86-10-62733710, E-mail: liuqc@cau.edu.cn
E-mail: liuqc@cau.edu.cn
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Cite this article:
ZHAO Ning, ZHAI Hong, YU Xiao-xia, LIU Zhe-sheng, HE Shao-zhen, LI Qiang, MA Dai-fu , LIU Qing-chang.
2013.
Development of SRAP Markers Linked to a Gene for Stem Nematode Resistance in Sweetpotato, Ipomoea batatas (L.) Lam.. Journal of Integrative Agriculture, 12(3): 414-419.
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| [1]Budak H, Shearman R C, Gaussoin R E, Dweikat I. 2004a.Application of sequence-related amplifiedpolymorphism markers for characterization of turfgrassspecies. HortScience, 39, 955-958[2]Budak H, Shearman R C, Parmaksiz I, Dweikat I. 2004b.Comparative analysis of seeded and vegetative biotypebuffalograsses based on phylogenetic relationshipusing ISSRs, SSRs, RAPDs, and SRAPs. Theoreticaland Applied Genetics, 109, 280-288[3]Budak H, Shearman R C, Parmaksiz I, Gaussion R E, RiordanT P, Dweikat I. 2004c. Molecular characterization ofbuffalograss germplasm using sequence-relatedamplified polymorphism markers. Theoretical andApplied Genetics, 108, 328-334[4]Darvari A, Soller M. 1994. Selective DNA pooling fordetermination of linkage between a molcular marker anda quantitative trait locus. Genetics, 138, 1365-1373[5]Dhir S K, Oglesby J, Bhagsari A S. 1998. Plant regenerationvia embryogenesis and transient gene expression insweetpotato protoplasts. Plant Cell Report, 17, 665-669[6]Ferriol M, Pico B, de Cordova P F, Nunez F. 2004. Moleculardiversity of a germplasm collection of squash(Cucurbita moschata) determined by SRAP and AFLPmarkers. Crop Science, 44, 653-664[7]Ferriol M, Pico B, Nuez F. 2003. Genetic diversity of agermplasm collection of Cucurbita pepo using SRAPand AFLP markers. Theoretical and Applied Genetics,107, 271-282[8]Jiang L, Yuan L, Zha X D, Wu F, Ma D F, Xie Y P, Li X Y,Wang Y. 2007. A RAPD marker linked to the resistanceDitylenchus destructor in sweetpotato. MolecularPlant Breeding, 5, 655-660[9]Jie Q, Jiang W, Li H, Zhai H, Ma D F, Xie Y P, Liu Q C.2008a. Inheritance analysis and SCAR marker of thegene for stem nematode resistance in sweetpotato,Ipomoea batatas (L.) Lam. Molecular Plant Breeding,6, 523-526[10]Jie Q, Li H, Zhai H, Wang Y P, Li Q, Ma D F, Xie Y P, Liu QC. 2008b. Development of AFLP markers linked to stemnematode resistance gene in sweetpotato (Ipomoeabatatas). Journal of Agricultural Biotechnology, 16,837-841[11]Jones A. 1965. Cytological observation and fertilitymeasurements of sweetpotato. Proceedings of theAmerican Society for Horticultural Science, 86, 527-537[12]Li A X, Wang Q M, Hou F Y, Zhang H Y, Zhang L M. 2008.Two SRAP markers linked to sweetpotato stemnematode resistance gene in sweetpotato. MolecularPlant Breeding, 6, 1204-1208[13]Li G, Quiros C F. 2001. Sequence-related amplifiedpolymorphism (SRAP), a new marker system based ona simple PCR reaction: its application to mapping andgene tagging in Brassica. Theoretical and Applied Genetics, 103, 455-461[14]Li G, Gao M, Yang B, Quiros C F. 2003. Gene for genealignment between the Brassica and Arabidopsisgenomes by direct transcriptome mapping. Theoreticaland Applied Genetics, 107, 168-180[15]Lin Z, He D, Zhang X, Nie Y, Guo X, Feng C, Stewart J M D.2005. Linkage map construction and mapping QTL forcotton fibre quality using SRAP, SSR and RAPD. PlantBreeding, 124, 180-187[16]Lin Z X, Zhang X L, Nie Y C. 2004. Evaluation of applicationof a new molecular marker SRAP on analysis of F2segregation population and genetic diversity in cotton.Acta Genetica Sinica, 31, 622-626 (in Chinese)[17]Lu S Y, Liu Q C, Li W J. 1998. Sweetpotato Breeding. ChinaAgriculture Press, Beijing. (in Chinese)Ma D F, Li H M, Xie Y P, Li X Y, Zhu C W, Jiang X M. 1997.Breeding of stem-nematode resistant varieties insweetpotato. Crops, 2, 15-16[18]Magoon M L, Krishnar R, Bai K V. 1970. Ctytologicevidence on the origin of sweetpotato. Theoretical andApplied Genetics, 40, 360-366[19]Michelmore R W, Paran I, Kesseli R V. 1991. Identificationof markers linked to disease resistance genes by bulkedsegregent analysis: a rapid method to detect markers inspecific genomic regions using segregating population.Proceedings of the American Society for HorticulturalScience, 88, 9828-9832[20]Riaz A, Li G, Quresh Z, Swati M S, Quiros C F. 2001. Geneticdiversity of oilseed Brassica napus inbred lines basedon sequence-related amplified polymorphism and itsrelation to hybrid performance. Plant Breeding, 120,411-415[21]Saghai-Maroof M A, Soliman K M, Jorgensen R A, AllardR W. 1984. Ribosomal DNA spacer-lengthpolymorphisms in barly: Mendelian inheritance,chromosomal location, and population dynamics.Proceedings of the National Academy of Sciences ofthe United States of America, 81, 8014-8018[22]Ukoskit K, Thompson P G, Watson C E, Watson Jr C E.1997. Identifying a randomly amplified polymorphicDNA (RAPD) markers linked to a gene for root knotnematode resistance in sweetpotato. Journal ofAmerican Society for Horticultural Science, 122, 818-821[23]Vos P, Hogers R, Bleeker M, Lee van de T, Horns M, FrijtersA, Pot J, Peleman J, Kuiper M, Zabeau M. 1995. AFLP:a new technique for DNA fingerprinting. Nucleic AcidsResearch, 23, 4407-4414[24]Xie Y Z, Yin Q H, Dai Q W, Qiu R L. 2004. Inheritance andbreeding for resistance to sweetpotato nematodes.Journal of Plant Genetic Resources, 5, 393-396[25]Zang N, Zhai H, Gao S, Chen W, He S Z, Liu Q C. 2009.Efficient production of transgenic plants using the bargene for herbicide resistance in sweetpotato. ScientiaHorticulturae, 122, 649-653 (in Chinese)[26]Zhou Z, Wang X, Ma D F, Li H M, Xie Y P, Li X Y. 2005.Identification of RAPD markers linked to stem-nematoderesistant gene in sweetpotato. Journal of AgriculturalBiothecnology, 13, 549-552. (in Chinese) |
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