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Journal of Integrative Agriculture  2013, Vol. 12 Issue (2): 209-217    DOI: 10.1016/S2095-3119(13)60220-8
Crop Genetics · Breeding · Germplasm Resources Advanced Online Publication | Current Issue | Archive | Adv Search |
Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis
 LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu
1.State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization, Minstry of Education/Key Laboratory
of Crop Genomics and Genetic Improvement, Minstry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
2.School of Life Sciences, Tsinghua University, Beijing 100084, P.R.China
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摘要  14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.

Abstract  14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.
Keywords:  wheat       Ta14-3-3       heterosis       gene expression  
Received: 15 April 2012   Accepted:
Fund: 

This work was financially supported by the National Natural Science Foundation of China (30600392, 30871529), the National “863” Program of China (2012AA10A309).

Corresponding Authors:  Correspondence YAO Ying-yin, Tel/Fax: +86-10-62731452, E-mail: yingyin@cau.edu.cn     E-mail:  yingyin@cau.edu.cn

Cite this article: 

LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu. 2013. Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis. Journal of Integrative Agriculture, 12(2): 209-217.

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