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| Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis |
| LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu |
1.State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization, Minstry of Education/Key Laboratory
of Crop Genomics and Genetic Improvement, Minstry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, P.R.China
2.School of Life Sciences, Tsinghua University, Beijing 100084, P.R.China |
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摘要 14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.
Abstract 14-3-3 proteins belong to a family of phosphoserine/threonine-binding modules and participate in a wide array of signal transduction and regulatory events. Our previous study demonstrated that Ta14-3-3 was significantly down-regulated in leaf and root tissues of hybrid wheat at the tillering stage. In this paper, three homoeologous Ta14-3-3 genes were cloned from common wheat (Triticum aestivum L., 2n=6x=42, AABBDD) and mapped on chromosomes 2A, 2B, and 2D, respectively. Transgenic Arabidopsis plants ectopically overexpressing Ta14-3-3 displayed shorter primary roots, delayed flowering and retarded growth rates, indicating that Ta14-3-3 acted as a growth inhibitor in Arabidopsis. In wheat, Ta14-3-3 was down-regulated in roots and leaves of hybrids as compared to their parental lines. We proposed that Ta14-3-3 proteins might regulate growth vigor in hybrid wheat.
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Received: 15 April 2012
Accepted:
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| Fund: This work was financially supported by the National Natural Science Foundation of China (30600392, 30871529), the National “863” Program of China (2012AA10A309). |
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Corresponding Authors:
Correspondence YAO Ying-yin, Tel/Fax: +86-10-62731452, E-mail: yingyin@cau.edu.cn
E-mail: yingyin@cau.edu.cn
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Cite this article:
LI Jing, SONG Su-sheng, ZHAO Yu-sheng, GUO Wei-wei, GUO Guang-hui, PENG Hui-ru, NI Zhong-fu.
2013.
Wheat 14-3-3 Protein Conferring Growth Retardation in Arabidopsis. Journal of Integrative Agriculture, 12(2): 209-217.
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