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Journal of Integrative Agriculture  2011, Vol. 10 Issue (11): 1653-1664    DOI: 10.1016/S1671-2927(11)60164-8
GENETICS & BREEDING · GERMPLASM RESOURCES · MOLECULAR GENETICS Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage 
 HU Biao-lin, FU Xue-qin, ZHANG Tao, WAN Yong, LI Xia, HUANG Yun-hong, DAI Liang-fang, LUO Xiang-dong , XIE Jian-kun
1.Rice Research Institute, Jiangxi Academy of Agricultural Sciences
2.College of Life Sciences, Jiangxi Normal University
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摘要  Drought stress is one of the major constraints to rice (Oryza sativa L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) Oryza rufipogon Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield via transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC1F5 of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.

Abstract  Drought stress is one of the major constraints to rice (Oryza sativa L.) production and yield stability especially in rainfed ecosystems and is getting worse as the climate changes worldwide. Dongxiang wild rice (DXWR) Oryza rufipogon Griff., contains drought resistant gene. Improving drought resistance of cultivars is crucial to increase and stabilize rice grain yield via transferring resistant gene from species related to rice. In this paper, four upland rice, sixty backcross inbred lines (BILs) derived from BC1F5 of R974//DXWR/R974, and their parents were employed to evaluate drought-resistance at seedling stage in the greenhouse. Nine traits were recorded for assessment of drought resistance, including maximum root length (MRL), number of roots (NR), shoot length (SL), dry root weight (DRW), fresh root weight (FRW), root relative water content (RRWC), leaf relative water content (LRWC), level for rolling leaf (LRL), and seedling survivability under repeat drought (SSRD). Using more than 88% of accumulative contribution resulted from the principal component analysis (PCA), the nine traits were classified into five independent principal components and the line 1949 showed the highest resistance. Analysis on the stepwise regression equation and correlation demonstrated that MRL, RN, FRW, and RRWC significantly influenced the drought resistance, thus could be used as comprehensive index for drought resistance at the seedling stage. Using the major gene plus polygene mixed inheritance model of quantitative traits, the inheritance of drought-resistance of BIL population at seedling stage was mostly controlled by two independent genes plus polygene. As a result, the DXWR could be precious resources for genetic improvement of drought resistance in cultivated rice.
Keywords:  Dongxiang wild rice (DXWR)      drought resistance      principal component analysis (PCA)      drought comprehensive index      seedling stage  
Received: 09 November 2010   Accepted:
Fund: 
This project was finiancially supported by the National Natural Science Fundation of China (30960189), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China, the project for Principle Research Topic of Jiangxi Education, China (GJJ08146), the Jiangxi Province Project for Principle Research Leader, China (020007), the Jiangxi Province Inviting Tender Project for Principle Research Topic, China (20068), the Natural Science Foundation of Jiangxi Province, China (2009GQN0068), and the Youth Foundation of Jiangxi Academy of Agricultural Sciences, China (2010CQN008).
Corresponding Authors:  Correspondence XIE Jian-kun, Professor, Mobile: 13707082911, E-mail: xiejiankun@yahoo.com      E-mail:  xiejiankun@yahoo.com
About author:  HU Biao-lin, MSc, Mobile: 13517911207, E-mail: hubiaolin992@yahoo.com.cn

Cite this article: 

HU Biao-lin, FU Xue-qin, ZHANG Tao, WAN Yong, LI Xia, HUANG Yun-hong, DAI Liang-fang, LUO Xiang-dong , XIE Jian-kun. 2011. Genetic Analysis on Characteristics to Measure Drought Resistance Using Dongxiang Wild Rice (Oryza rufupogon Griff.) and Its Derived Backcross Inbred Lines Population at Seedling Stage . Journal of Integrative Agriculture, 10(11): 1653-1664.

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