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Journal of Integrative Agriculture  2012, Vol. 12 Issue (1): 100-108    DOI: 10.1016/S1671-2927(00)8522
PLANT PROTECTION Advanced Online Publication | Current Issue | Archive | Adv Search |
Expression of Nitrilases in Brassica juncea var. tumida Tsen in Root Galls Caused by Plasmodiophora brassicae
 LIU Yan, YIN You-ping, WANG Zhong-kang , LUO Yuan-li
1.Bioengineering College, Chongqing University, Chongqing 400030, P.R.China
2.Fuling Agricultural Science Institute of Chongqing, Chongqing 400030, P.R.China
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摘要  Five commonly-used reference genes: ACT (actin), UBE (ubiquitin-conjugating enzyme), RPL2 (ribosomal protein L2), BRP II (RNA polymerase II subunit), and NADH (nicotinamide adenine dinucleotide) were examined using geNorm software as reference genes for RT-qPCR. Among the tested reference genes, ACT and UBE were the most stable in all samples. In parallel, expression analysis of nitrilases in Brassica juncea var. tumida, was performed to preliminarily investigate the molecular interactions between nitrilase and clubroot development at 10, 15, 20, 25, 30, and 40 d postinoculation (dpi) with a suspension of resting spores of Plasmodiophora brassicae. The results showed that different gene expressions of nitrilases were regulated during the initial periods of clubroot development. The expression level of BjNIT1 increased sharply from 20 to 40 dpi in infected roots while there were no remarkable changes in healthy roots. From 15 to 30 dpi, the expression levels of BjNIT2 and BjNIT4 in infected roots were lower than those in non-infected roots. Finally, BjNIT2 in treatment was down approximately to control at 40 dpi. Our results suggest that BjNIT1, which promoted overproductions of auxin, might be involved in P. brassicae infection of B. juncea.

Abstract  Five commonly-used reference genes: ACT (actin), UBE (ubiquitin-conjugating enzyme), RPL2 (ribosomal protein L2), BRP II (RNA polymerase II subunit), and NADH (nicotinamide adenine dinucleotide) were examined using geNorm software as reference genes for RT-qPCR. Among the tested reference genes, ACT and UBE were the most stable in all samples. In parallel, expression analysis of nitrilases in Brassica juncea var. tumida, was performed to preliminarily investigate the molecular interactions between nitrilase and clubroot development at 10, 15, 20, 25, 30, and 40 d postinoculation (dpi) with a suspension of resting spores of Plasmodiophora brassicae. The results showed that different gene expressions of nitrilases were regulated during the initial periods of clubroot development. The expression level of BjNIT1 increased sharply from 20 to 40 dpi in infected roots while there were no remarkable changes in healthy roots. From 15 to 30 dpi, the expression levels of BjNIT2 and BjNIT4 in infected roots were lower than those in non-infected roots. Finally, BjNIT2 in treatment was down approximately to control at 40 dpi. Our results suggest that BjNIT1, which promoted overproductions of auxin, might be involved in P. brassicae infection of B. juncea.
Keywords:  Brassica juncea var. tumida Tsen      reference gene      Plasmodiophora brassicae      clubroot  
Received: 12 October 2010   Accepted:
Fund: 

This work was financially supported by the Natural Science Foundation of Chongqing Science and Technology Commission, China (2008BB1370).

Corresponding Authors:  Correspondence WANG Zhong-kang, Tel: +86-23-65120489, E-mail: w-zk@163.com     E-mail:  w-zk@163.com
About author:  LIU Yan, Mobile: 15233309265, E-mail: liuyan9891@126.com

Cite this article: 

LIU Yan, YIN You-ping, WANG Zhong-kang , LUO Yuan-li. 2012. Expression of Nitrilases in Brassica juncea var. tumida Tsen in Root Galls Caused by Plasmodiophora brassicae. Journal of Integrative Agriculture, 12(1): 100-108.

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