Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions

doi:10.1016/S2095-3119(13)60687-5

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (12): 2678-2689.DOI: 10.1016/S2095-3119(13)60687-5

Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions

LU Zeng-bin, HAN Nai-shun, TIAN Jun-ce, PENG Yu-fa, HU Cui, GUO Yu-yuan, SHEN Zhicheng, YE Gong-yin

1、State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences,
Zhejiang University, Hangzhou 310058, P.R.China
2、State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Institute of Plant Protection and Microbiology,
Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
3、State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China

收稿日期:2013-10-15 出版日期:2014-12-01 发布日期:2014-12-10
通讯作者: YE Gong-yin, E-mail: chu@zju.edu.cn
作者简介:LU Zeng-bin, E-mail: luzengbin12345@163.com
基金资助:
Financial support was provided by the Special Research Projects for Developing Transgenic Plants, China (2013ZX08011-001) and China National Science Fund for Innovative Research Group of Biological Control (31021003) as well as the National Basic Research Progarm of China (973 Program, 2007CB109202).

Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions

LU Zeng-bin, HAN Nai-shun, TIAN Jun-ce, PENG Yu-fa, HU Cui, GUO Yu-yuan, SHEN Zhicheng, YE Gong-yin

1、State Key Laboratory of Rice Biology/Key Laboratory of Agricultural Entomology, Ministry of Agriculture/Institute of Insect Sciences,
Zhejiang University, Hangzhou 310058, P.R.China
2、State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control/Institute of Plant Protection and Microbiology,
Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
3、State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences,
Beijing 100193, P.R.China

Received:2013-10-15 Online:2014-12-01 Published:2014-12-10
Contact: YE Gong-yin, E-mail: chu@zju.edu.cn
About author:LU Zeng-bin, E-mail: luzengbin12345@163.com
Supported by:
Financial support was provided by the Special Research Projects for Developing Transgenic Plants, China (2013ZX08011-001) and China National Science Fund for Innovative Research Group of Biological Control (31021003) as well as the National Basic Research Progarm of China (973 Program, 2007CB109202).

摘要/Abstract

摘要： Numerous Bt rice lines expressing Cry protein derived from Bacillus thuringiensis Berliner (Bt) have been developed since 1989. However, the potential risks posed by Bt rice on non-target organisms still remain debate. The white-backed planthopper (WBPH), Sogatella furcifera (Horváth), is one of the most economically important insect pests of rice in Asian countries and also one of the main non-target herbivores of transgenic rice. In the current study, impacts of transgenic cry1Ab/vip3H+epsps rice (G6H1) with both insect and herbicide resistance on WBPH were evaluated to ascertain whether this transgenic rice line had potential risks for this sap-sucking pest under laboratory and field conditions. The laboratory results showed that no significant difference in egg developmental duration, nymphal survival rate and female fecundity was found for WBPH between G6H1 and its non-transgenic isoline (XS110). However, the development duration of nymphs was significantly shorter and female longevity significantly longer when WBPH fed on G6H1 by comparison with those on its control. To verify the results found in laboratory, a 3-yr field trial was conducted to monitor WBPH population using both the vacuum-suction machine and beat plate methods. Although the seasonal density of WBPH nymphs and total density of nymphs and adults were not significantly affected by transgenic rice regardless of the sampling methods, the seasonal density of WBPH adults in transgenic rice plots was slightly lower than that in the control when using the vacuum-suction machine. Based on these results both from laboratory and field, it is clear that our tested transgenic rice line will not lead higher population of WBPH. However, long-term field experiments to monitor the population dynamics of WPBH at large scale need to be conducted to confirm the present conclusions in future.

关键词: transgenic rice , Bacillus thuringiensis , cry1Ab , vip3H , Sogatella furcifera , biological parameters , population dynamics

Abstract: Numerous Bt rice lines expressing Cry protein derived from Bacillus thuringiensis Berliner (Bt) have been developed since 1989. However, the potential risks posed by Bt rice on non-target organisms still remain debate. The white-backed planthopper (WBPH), Sogatella furcifera (Horváth), is one of the most economically important insect pests of rice in Asian countries and also one of the main non-target herbivores of transgenic rice. In the current study, impacts of transgenic cry1Ab/vip3H+epsps rice (G6H1) with both insect and herbicide resistance on WBPH were evaluated to ascertain whether this transgenic rice line had potential risks for this sap-sucking pest under laboratory and field conditions. The laboratory results showed that no significant difference in egg developmental duration, nymphal survival rate and female fecundity was found for WBPH between G6H1 and its non-transgenic isoline (XS110). However, the development duration of nymphs was significantly shorter and female longevity significantly longer when WBPH fed on G6H1 by comparison with those on its control. To verify the results found in laboratory, a 3-yr field trial was conducted to monitor WBPH population using both the vacuum-suction machine and beat plate methods. Although the seasonal density of WBPH nymphs and total density of nymphs and adults were not significantly affected by transgenic rice regardless of the sampling methods, the seasonal density of WBPH adults in transgenic rice plots was slightly lower than that in the control when using the vacuum-suction machine. Based on these results both from laboratory and field, it is clear that our tested transgenic rice line will not lead higher population of WBPH. However, long-term field experiments to monitor the population dynamics of WPBH at large scale need to be conducted to confirm the present conclusions in future.

Key words: transgenic rice , Bacillus thuringiensis , cry1Ab , vip3H , Sogatella furcifera , biological parameters , population dynamics

LU Zeng-bin, HAN Nai-shun, TIAN Jun-ce, PENG Yu-fa, HU Cui, GUO Yu-yuan, SHEN Zhicheng, YE Gong-yin. Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions[J]. Journal of Integrative Agriculture, 2014, 13(12): 2678-2689.

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Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions

Transgenic cry1Ab/vip3H+epsps Rice with Insect and Herbicide Resistance Acted No Adverse Impacts on the Population Growth of a Non-Target Herbivore, the White-Backed Planthopper, Under Laboratory and Field Conditions

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